Abstract: The present disclosure provides engineered Saccharomyces cerevisiae strains for production of vanillylamine and capsaicin, and a construction method and use thereof, and belongs to the technical field of bioengineering. In the present disclosure, a synthesis pathway of vanillylamine is expressed heterologously in Saccharomyces cerevisiae for the first time, and biosynthesis of the vanillylamine is realized for the first time. After the optimization of precursors supply and the optimization of S-adenosyl-L-methionine (SAM) cycle, a yield of the vanillylamine reaches 16.48 g/L, and a synthesis efficiency of the vanillylamine is increased by nearly 155%. This is the highest optimization efficiency obtained by optimizing the SAM cofactor strategy so far.

Abstract: The present invention discloses a synthesis method of lactide by confinement effect catalysis of crystalline porous polymer material, wherein the method comprising: (I) synthesis of catalyst; (II) synthesis of lactide by confinement effect catalysis; and (III) purification of lactide. In the present invention, a yield of L-lactide by catalysis of L-lactic acid by crystalline polymers is as high as 85.6%, which is 10% higher than the yield of lactide by H-? molecular sieve reported in documents currently available; it is easy to prepare the crystalline porous polymer material catalyst, which is environmental friendly, has a high yield and is recyclable, for consecutive 7 times the catalysis yield is maintained to be higher than 70%, and catalysis yield conservation rate is far higher than catalysis effects of catalysts reported in documents currently available.

Abstract: A method for processing an operating state of a high-pressure manifold, includes: determining a connection diagram of the high-pressure manifold, and the connection diagram is a line diagram formed by a plurality of device nodes; obtaining manifold data from a start node to an end node in the line diagram, and the manifold data includes flow parameters of a fluid in the high-pressure manifold; and determining the operating state of the high-pressure manifold according to the manifold data.

Abstract: The present invention discloses a synthesis method of lactide by confinement effect catalysis of crystalline porous polymer material, wherein the method comprising: (I) synthesis of catalyst; (II) synthesis of lactide by confinement effect catalysis; and (III) purification of lactide. In the present invention, a yield of L-lactide by catalysis of L-lactic acid by crystalline polymers is as high as 85.6%, which is 10% higher than the yield of lactide by H-? molecular sieve reported in documents currently available; it is easy to prepare the crystalline porous polymer material catalyst, which is environmental friendly, has a high yield and is recyclable, for consecutive 7 times the catalysis yield is maintained to be higher than 70%, and catalysis yield conservation rate is far higher than catalysis effects of catalysts reported in documents currently available.