Abstract: The disclosure provides an active peptide with an anti-lipid oxidation function and a preparation method and application thereof and belongs to the technical field of plant-derived biologically active peptides. In the disclosure, oil processing by-products, namely oil crops after oil extraction, are used as the raw materials, and the raw materials are subjected to the steps of protein extraction, infrared pretreatment, proteolysis, freeze-drying, lipophilic part extraction, vacuum concentration and drying to prepare an anti-lipid oxidation peptide having the functional characteristics of scavenging DPPH free radicals, chelating metal ions, inhibiting lipid peroxidation, prolonging vegetable oil oxidation induction time, improving emulsion stability and the like.

Abstract: Disclosed is a preparation method for collagen hydrogel and belongs to the technical field of gene engineering. On the basis of E3 and K3 heterologous ?-helices at an N end and a C end, a continuous Gly-Xaa-Yaa triplet collagen sequence is inserted therebetween to form a three-segment chimeric collagen E3-collagen-K3 mode. Self-assembly is driven by the interaction of the E3 and K3 heterologous ?-helices at the N end and the C end to form the end-to-end reticulated porous collagen hydrogel. The method of the disclosure can prepare collagen with a clean source, the collagen hydrogel can be formed by self-assembly of the collagen, the preparation process is simple, and the collagen hydrogel can be produced on a large scale at low cost and has a wide application prospect in the field of biological materials.

Abstract: The disclosure discloses a preparation of type I collagen-like fibers and a method for regulating and controlling the periodic length of fiber stripes thereof, belonging to the technical field of genetic engineering. The disclosure produces a three-segment chimeric collagen P-CL-P pattern by inserting a continuous Gly-Xaa-Yaa triplet collagen sequence in the middle based on the N- and C-terminal (GPP)n sequences. The self-assembly is driven by the interaction between the N- and C-terminal (GPP)n triple helixes to form banded fibers with periodic bright and dark stripes. According to the method of the disclosure, a fiber from a clean source, which can self-assemble to form periodic bright and dark stripes can be prepared, the structure of which is similar to type I collagen, the preparation process is simple, the collagen fiber with low cost can be produced on a large scale, and the method has broad application prospects in the field of biological materials.

Abstract: Disclosed is a preparation method for collagen hydrogel and belongs to the technical field of gene engineering. On the basis of E3 and K3 heterologous ?-helices at an N end and a C end, a continuous Gly-Xaa-Yaa triplet collagen sequence is inserted therebetween to form a three-segment chimeric collagen E3-collagen-K3 mode. Self-assembly is driven by the interaction of the E3 and K3 heterologous ?-helices at the N end and the C end to form the end-to-end reticulated porous collagen hydrogel. The method of the disclosure can prepare collagen with a clean source, the collagen hydrogel can be formed by self-assembly of the collagen, the preparation process is simple, and the collagen hydrogel can be produced on a large scale at low cost and has a wide application prospect in the field of biological materials.

Abstract: The disclosure discloses a preparation of type I collagen-like fibers and a method for regulating and controlling the periodic length of fiber stripes thereof, belonging to the technical field of genetic engineering. The disclosure produces a three-segment chimeric collagen P-CL-P pattern by inserting a continuous Gly-Xaa-Yaa triplet collagen sequence in the middle based on the N- and C-terminal (GPP)n sequences. The self-assembly is driven by the interaction between the N- and C-terminal (GPP)n triple helixes to form banded fibers with periodic bright and dark stripes. According to the method of the disclosure, a fiber from a clean source, which can self-assemble to form periodic bright and dark stripes can be prepared, the structure of which is similar to type I collagen, the preparation process is simple, the collagen fiber with low cost can be produced on a large scale, and the method has broad application prospects in the field of biological materials.