Abstract: A Brillouin modality can be supplemented by an auxiliary modality, such as an optical imaging modality or a spectroscopy modality. In some embodiments, the auxiliary modality can be used to guide the Brillouin measurement to a desired region of interest, so that acquisition times for the Brillouin measurement can be reduced as compared to interrogating the entire sample. The auxiliary modality may have an acquisition speed faster than that of the Brillouin modality. In some embodiment, the auxiliary modality determines a composition of materials within a voxel in the sample interrogated by the Brillouin modality. Using the information provided by the auxiliary modality, the Brillouin signatures corresponding to the materials within the voxel can be unmixed, thereby providing a more accurate measurement of the sample.

Abstract: A line-scan Brillouin microscopy apparatus is configured to operate in a coaxial configuration. The line-scan Brillouin microscopy apparatus includes an illumination source that provides a P polarized illumination light beam for illuminating a sample. A first optical assembly provides the P polarized illumination light beam to the sample and collects initial Brillouin scattered light from the sample. The first optical assembly includes an optical component that converts the initial Brillouin scattered light to S polarized Brillouin scattered light. A second optical assembly is configured to receive the S polarized Brillouin scattered light from the first optical assembly. Characteristically, the second optical assembly is configured to induce a spectral dispersion. A detection unit is configured to detect a spatio-spectral pattern of the initial Brillouin scattered light. Advantageously, multiple points of the sample along P polarized illumination light beam are measured simultaneously.

Abstract: A multimodal optical technique that can measure the mechanical, optical, and acoustical properties of the sample at microscopic resolution, which is based on the integration of a Brillouin microscope and a photoacoustic (PA) microscopy is provided. The multimodal technique not only can acquire co-registered Brillouin and PA signals of the sample but also allows us to utilize the sound speed measurements by PA to quantify the sample's refractive index, which is an essential property of the material and cannot be measured by either technique individually. We demonstrated the colocalization of Brillouin and time-resolved PA signals by measuring the interface of kerosene and 1% CuSO4 aqueous solution. In addition, we measured the refractive index of saline solutions with a precision of 0.003 and validated the result against published data. This multimodal modality could open new way for charactering biological cell and tissue in physiological and pathological conditions.

Abstract: An apparatus for super-resolution Brillouin microscopy includes a probe laser that emits a first laser beam and a first objective lens that focuses the first laser beam onto a sample. The apparatus further includes a pump laser that emits a second laser beam and a second quarter-wave plate that receives the second laser beam. The apparatus further includes a depletion laser that emits a third laser beam that passes through a phase plate to modify its wavefront phase such that the third laser beam has a donut shape, and a second objective lens that focuses the second laser beam and the third laser beam onto the sample. Characteristically, the beam spot from the depletion laser is overlaid with the Gaussian-shape beam spots of the first laser beam and the second laser beam at the same focal plane. A detector is configured to detect a stimulated Brillouin gain signal and a stimulated Brillouin loss signal.

Abstract: The present disclosure relates to a Cu—Zn-SSZ-13 molecular sieve composite catalyst and a preparation method thereof. Firstly, in situ synthesis is conducted by an one-step method and the template agent TMAdaOH is removed. Then a processed diatomite filter aid containing a SSZ-13 molecular sieve seed is added to improve the molecular sieve yield and thus reduce the cost. Finally, the Cu—Zn-SSZ-13 molecular sieve composite catalyst is prepared by substituting ammonium nitrate with ammonium dihydrogen phosphate or diammonium hydrogen phosphate through a counter-ion exchange method. The reaction performance of the catalyst in a temperature range of 100-600° C. is better than that of a SSZ-13 catalyst prepared by an ion exchange method and a Cu-SSZ-13 molecular sieve catalyst prepared by an one-step method, and the yield of the synthetic object is increased by 40%.

Abstract: The present disclosure discloses a method for preparing a hierarchical porous material for a molecular sieve with an MFI structure. In the present disclosure, porous silicon dioxide is used as a carrier on a surface of which a nano zeolite seed is loaded, and then subjected to treatment with a secondary growth compound fluid and hydrothermal crystallization, so that a nano zeolite molecular sieve membrane is further grown on the surface of the porous silicon dioxide. The specific surface area and pore volume of the hierarchical porous material for the molecular sieve with the MFI structure prepared by the present disclosure are greatly improved compared with those of the original porous silicon dioxide. The material not only has the macroporous structure of the porous silicon dioxide, but also incorporates micropores of the molecular sieve itself and mesopores formed by molecular sieve agglomeration.

Abstract: A Brillouin modality can be supplemented by an auxiliary modality, such as an optical imaging modality or a spectroscopy modality. In some embodiments, the auxiliary modality can be used to guide the Brillouin measurement to a desired region of interest, so that acquisition times for the Brillouin measurement can be reduced as compared to interrogating the entire sample. The auxiliary modality may have an acquisition speed faster than that of the Brillouin modality. In some embodiment, the auxiliary modality determines a composition of materials within a voxel in the sample interrogated by the Brillouin modality. Using the information provided by the auxiliary modality, the Brillouin signatures corresponding to the materials within the voxel can be unmixed, thereby providing a more accurate measurement of the sample.

Abstract: A Brillouin modality can be supplemented by an auxiliary modality, such as an optical imaging modality or a spectroscopy modality. In some embodiments, the auxiliary modality can be used to guide the Brillouin measurement to a desired region of interest, so that acquisition times for the Brillouin measurement can be reduced as compared to interrogating the entire sample. The auxiliary modality may have an acquisition speed faster than that of the Brillouin modality. In some embodiment, the auxiliary modality determines a composition of materials within a voxel in the sample interrogated by the Brillouin modality. Using the information provided by the auxiliary modality, the Brillouin signatures corresponding to the materials within the voxel can be unmixed, thereby providing a more accurate measurement of the sample.

Abstract: A Brillouin modality can be supplemented by an auxiliary modality, such as an optical imaging modality or a spectroscopy modality. In some embodiments, the auxiliary modality can be used to guide the Brillouin measurement to a desired region of interest, so that acquisition times for the Brillouin measurement can be reduced as compared to interrogating the entire sample. The auxiliary modality may have an acquisition speed faster than that of the Brillouin modality. In some embodiment, the auxiliary modality determines a composition of materials within a voxel in the sample interrogated by the Brillouin modality. Using the information provided by the auxiliary modality, the Brillouin signatures corresponding to the materials within the voxel can be unmixed, thereby providing a more accurate measurement of the sample.

Abstract: The present invention relates to a method and system for analyzing mechanical signatures of a plurality of cells for metastatic detection. Specifically, a data set characterized by at least one metric (such as Brillouin frequency shift and/or Brillouin linewidth) representing a cell mechanical signature is acquired for the plurality of cells by using a label-free Brillouin spectroscopy. A merit function is calculated based on one or more statistical characteristics of the data set, such as sensitivity and specificity. Then, the plurality of cells can be classified to detect metastatic cells based on mechanical signatures provided by the data set and an optimal metric value delivering maximum to the merit function.

Abstract: The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.

Abstract: The present invention relates to a method and system for identifying mechanical properties of a cell nucleus through a label-free cell analysis based on Brillouin light scattering techniques. The present application additionally provides a method and system for identifying cancerous cells based on mechanical properties of the cell nucleus.

Abstract: The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.

Abstract: The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.

Abstract: The present invention relates to a method and system for analyzing mechanical signatures of a plurality of cells for metastatic detection. Specifically, a data set characterized by at least one metric (such as Brillouin frequency shift and/or Brillouin linewidth) representing a cell mechanical signature is acquired for the plurality of cells by using a label-free Brillouin spectroscopy. A merit function is calculated based on one or more statistical characteristics of the data set, such as sensitivity and specificity. Then, the plurality of cells can be classified to detect metastatic cells based on mechanical signatures provided by the data set and an optimal metric value delivering maximum to the merit function.

Abstract: The present invention relates to a method and system for a label-free cell analysis based on Brillouin light scattering techniques. Combined with microfluidic technologies according to the present invention, Brillouin spectroscopy constitutes a powerful tool to analyze physical properties of cells in a contactless non-disturbing manner. Specifically, subcellular mechanical information can be obtained by analyzing the Brillouin spectrum of a cell. Furthermore, a novel configuration of Brillouin spectroscopy is provided to enable simultaneous analysis of multiple points in a cell sample.