Abstract: Primers, a kit and a method for detecting African swine fever virus are provided, which relates to virus detection technologies. The primers are used to detect vp72 gene of African swine fever virus, and nucleotide sequences thereof are shown in SEQ ID NO: 6 and SEQ ID NO: 7. The kit includes the primers. The method uses the primers or the kit to perform dye fluorescence quantitative polymerase chain reaction (PCR) on genomic DNA of a sample to be detected. This method has strong specificity, high sensitivity, good reproducibility, and lower cost than the TaqMan probe method.

Abstract: A use of a canopy fibroblast growth factor signaling regulator 3 (CNPY3) protein as a target for treating of dengue fever is provided belongs to the technical field of antiviral drugs. An important host protein CNPY3 related to dengue virus infection is identified. A host gene is significantly down-regulated in blood of dengue patients and in dengue virus (DENV) infected dendritic cells and THP-1 cells. Its expression is negatively correlated with the progression of dengue disease and positively correlated with the expression of most Toll-like receptors. Down-regulation of the host gene inhibits the production of IFN-? and the expression of ISGs in THP-1 cells, promoting DENV-2 infection. Up-regulation of the host gene expression in Vero and HEK 293T cells inhibits DENV-2 infection. It indicates that CNPY3 participates in an innate immune response signaling pathway, has the effect of anti-dengue virus, and is a potential therapeutic target for dengue fever.