Abstract: The present invention relates to a device and method for visual threshold measurement, which belongs to the field of optical precision measurement technology. The device comprises a first uniform plane light source system, a second uniform plane light source system, a first primary color adjusting system, a second primary color adjusting system, a first complementary color adjusting system, a second complementary color adjusting system, a first color mixture system, a second color mixture system, and a beam splitting system. The device can generate four observation targets with variable brightness (illumination), contrast, color difference, and periodic pattern frequency, by using a four-integrating sphere light source system and by multiplexing the color adjusting systems, the color mixture systems, and subsequent light paths, so as to implement multi-parameter measurement for a set of visual thresholds.