Abstract: Provided is a high-efficiency expression vector of a circular ribonucleic acid (circRNA). The high-efficiency expression vector is an expression framework with a total length of only 173 bp and having a nucleotide sequence shown in SEQ ID NO: 1, and includes an upstream frame sequence of 92 bp, a downstream frame sequence of 75 bp, and a restriction site EcoR I located between the upstream and downstream frame sequences for inserting a gene to be circularized. In the present disclosure, the high-efficiency expression vector of a circRNA can enable an exogenously-inserted sequence to efficiently express the circRNA, thus providing an efficient expression tool for obtaining the circRNA. On the premise of ensuring efficient circularization of the circRNA, lengths of upstream and downstream flanking sequences required for the circularization have been highly optimized to further reduce the complexity of splicing by-products caused by the flanking sequences.

Abstract: The present disclosure provides a pseudorabies virus (PRV) expressing a porcine circovirus 2 (PCV2) capsid protein in its envelope, and use thereof. In the present disclosure, an attenuated PRV vaccine strain is used as a vector to express an exogenous immunogen. The exogenous immunogen only replaces an extracellular domain of a non-essential envelope protein of the PRV, while retaining a transmembrane domain and an intracellular domain of an original envelope protein, thereby allowing the expression of one or more exogenous immunogens on the viral envelope without changing genes of other PRV autoimmunogens. During the recombinant PRV particle being recognized by the body's immune system, the host's immune system recognizes all immunogens (exogenous immunogens and PRV autoimmunogens) on the recombinant PRV particle and then initiates an immune response, thus exerting a protective effect of a bivalent vaccine or a polyvalent vaccine based on the recombinant PRV particle.

Abstract: An automatic pool cleaner includes a housing, a filtering module, and an out-of-water detection module. The housing defines a water inlet and a water outlet; The filtering module is configured to filter fluid from the water inlet to provide filtered fluid. The out-of-water detection module comprises a floating unit and a sensing unit, which can make the floating unit move between the first and the second positions according to whether the pool cleaner is above the waterline. The sensing unit identifies the position of the floating unit and outputs a first signal or a second signal to the pool cleaner, thereby determining whether the pool cleaner is above the water surface.

Abstract: The present invention discloses a type II Pseudorabies virus attenuated strain and its preparation method and application. The attenuated strain of pseudorabies virus is gE/TK-double-deficient strain, which is named as PRV-HD/c strain of PRV dual-deletion strain, and the accession number is CGMCC No. 14325. The attenuated strain of the pseudorabies virus of the present invention is obtained from the newly isolated strain of pseudorabies virus type II after deletion of the gE and TK double genes and has reduced pathogenicity and strong immunogenicity and is inactivated by the attenuated strain of pseudorabies virus vaccines or live attenuated vaccines, which can provide effective immunity to PRV susceptible animals such as pigs and mice.

Abstract: The present invention discloses a type II Pseudorabies virus attenuated strain and its preparation method and application. The attenuated strain of pseudorabies virus is gE/TK-double-deficient strain, which is named as PRV-HD/c strain of PRV dual-deletion strain, and the accession number is CGMCC No. 14325. The attenuated strain of the pseudorabies virus of the present invention is obtained from the newly isolated strain of pseudorabies virus type II after deletion of the gE and TK double genes and has reduced pathogenicity and strong immunogenicity and is inactivated by the attenuated strain of pseudorabies virus vaccines or live attenuated vaccines, which can provide effective immunity to PRV susceptible animals such as pigs and mice.