Abstract: A method of producing a protein of interest (POI) by culturing a recombinant eukaryotic cell line comprising an expression construct comprising a regulatable promoter and a nucleic acid molecule encoding a POI under the transcriptional control of said promoter, comprising the steps a) cultivating the cell line with a basal carbon source repressing the promoter, b) cultivating the cell line with a limited amount of a supplemental carbon source de-repressing the promoter to induce production of the POI at a transcription rate of at least 15% as compared to the native pGAP promoter, and c) producing and recovering the POI; and further an isolated regulatable promoter and a respective expression system.

Abstract: The invention relates to a folding card 1 with a cover 2 that is connected to a back 3 by at least one fold line F.sub.1 and with an internal folding system 4, that is connected at least to the inside surface 3a of the back 3 and can assume a three-dimensional form when the card is unfolded. In order to achieve a particularly effective and optimal effect, the internal folding system 4 consists of at least two superimposed frame elements that change their relative positions when the card is unfolded and whose windows are of differing sizes, whereby the size of the windows increases in the order of the successively overlapping frame elements. The largest window is the one located furthest from the back.