Patents by Inventor Joan E. Brooks

Joan E. Brooks has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for cloning and producing the BglII restriction endonuclease and modification methylase
    Patent number: 5434068
    Abstract: The present invention is directed to a method for cloning and producing the BglII endonuclease by: 1) introducing the restriction endonuclease gene from Bacillus globigii into a host whereby the restriction gene is expressed; 2) fermenting the host which contains the plasmid encoding and expressing the BglII restriction endonuclease activity; and 3) purifying the BglII restriction endonuclease from the fermented host which contains the plasmid encoding and expressing the BglII restriction endonuclease.
    Type: Grant
    Filed: April 9, 1993
    Date of Patent: July 18, 1995
    Assignee: New England Biolabs, Inc.
    Inventors: Joan E. Brooks, Daniel F. Heiter, Brian P. Anton
  • Method for producing the DdeI restriction endonuclease and methylase
    Patent number: 5354680
    Abstract: A two-step method for cloning the DdeI restriction modification system is provided which comprises introducing the DdeI modification or methylase gene into a host cell and expressing the gene to protect the DdeI host cell followed by introducing the DdeI restriction or endonuclese gene into the host.
    Type: Grant
    Filed: November 26, 1991
    Date of Patent: October 11, 1994
    Assignee: New England Biolabs, Inc.
    Inventors: Joan E. Brooks, Kimberly H. O'Donnell
  • Method for cloning restriction modification system
    Patent number: 5320957
    Abstract: A two-step method for cloning restriction modification systems is provided which comprises introducing the modification or methylase gene into a host cell and expressing the gene therein to protect the host cell followed by introducing the restriction or endonuclease gene into the host whereby the restriction gene is expressed at lower levels relative to the modification gene. Related methods for producing restriction enzymes is also disclosed as well as clones containing the BamI and DdeI restriction genes.
    Type: Grant
    Filed: August 11, 1992
    Date of Patent: June 14, 1994
    Assignee: New England Biolabs, Inc.
    Inventors: Joan E. Brooks, Kimberly A. Howard
  • Method for producing the Bamhi restriction endonuclease and methylase
    Patent number: 5137823
    Abstract: A two-step method for cloning the BamHI restriction modification system is provided which comprises introducing the BamHI modification or methylase gene into a host cell and expressing the gene to protect the host cell followed by introducing the BamHI restriction or endonuclease gene into the host.
    Type: Grant
    Filed: December 14, 1989
    Date of Patent: August 11, 1992
    Assignee: New England Biolabs, Inc.
    Inventor: Joan E. Brooks
  • Method for producing the Eag I restriction endonuclease and methylase
    Patent number: 4996151
    Abstract: The present invention is directed to a method for cloning and producing the Eag I restriction endonuclease by (1) introducing the restriction endonuclease gene from E. agglomerans ATCC into a host whereby the restriction gene is expressed; (2) fermenting the host which contains the vector encoding and expressing the Eag I restriction endonuclease, and (3) purifying the Eag I restriction endonuclease from the fermented host which contains the vector encoding and expressing the Eag I restriction endonuclease activity.
    Type: Grant
    Filed: May 19, 1988
    Date of Patent: February 26, 1991
    Assignee: New England Biolabs, Inc.
    Inventors: Joan E. Brooks, Laura A. Sznyter