Abstract: Method and test kit for detecting Clq-containing complexes in human serum containing native serum Cl. The method uses a monoclonal antibody which selectively reacts with human Clq in the presence of native human serum Cl. Preparation of hybridomas generating such antibodies is also disclosed. The method is applicable to detection of autoimmune diseases and AIDS.

Abstract: Method for detecting circulating immune complexes containing endogenously bound Clq. Capillary tubes are filled with a mixture of Clq coated GPO reagent cells, rabbit anti-Clq antibodies, and precipitate from the biological fluid sample containing immune complexes. The mixture is allowed to react and the tubes read for the presence or absence of agglutination indicative of the absence or presence of said immune complexes respectively.

Abstract: Kit for detecting circulating immune complexes containing endogenously bound Clq. Capillary tubes are filled with a mixture of Clq coated GPO reagent cells, rabbit anti-Clq antibodies, and precipitate from the biological fluid sample containing immune complexes. The mixture is allowed to react and the tubes read for the presence or absence of agglutination indicative of the absence or presence of said immune complexes respectively.

Abstract: A two-step process for achieving a covalent bond between latex polymers and immunological substances, the first of which involves reacting latex particles having a reactive carboxyl group with a diamine in the presence of an appropriate carbodiimide as the condensing agent. The resulting product, which can be designated as the modified latex, is an amide terminating in primary or secondary amino groups. This product is then reacted with the appropriate immunological reagent such as an antigen or antibody modified if necessary to contain amino groups, in the presence of a bifunctional aldehyde to result in the condensation of the amino groups of the immunological reagent to the amino groups of the modified latex via an intermediate aldehyde bridging moiety.