Patents by Inventor Joaquin A. Umana
Joaquin A. Umana has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20220410076Abstract: Disclosed are methods for coupling a ligand to a composite material. Covalent bonds are formed between functionalized composite materials and ligands as a ligand solution flows through or across the composite materials. The composite materials are useful as chromatographic separation media.Type: ApplicationFiled: November 20, 2020Publication date: December 29, 2022Inventors: Matthew T. Stone, Santosh Rahane, Joaquin A. Umana, Gary Skarja, Elena N. Komkova, Melissa Vandersluis, Amro Ragheb
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Patent number: 11305271Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).Type: GrantFiled: August 15, 2017Date of Patent: April 19, 2022Assignee: EMD Millipore CorporationInventors: David Yavorsky, John Amara, Joaquin Umana, William Cataldo, Mikhail Kozlov, Matthew Stone
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Publication number: 20180215786Abstract: The present invention provides novel compositions and methods for removal of protein aggregates from a sample in a flow-through mode.Type: ApplicationFiled: March 14, 2018Publication date: August 2, 2018Inventors: Mikhail Kozlov, William Cataldo, Ajish Potty, Kevin Galipeau, James Hamzik, Joaquin Umana, Lars Peeck
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Publication number: 20180085743Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).Type: ApplicationFiled: August 15, 2017Publication date: March 29, 2018Inventors: David Yavorsky, John Amara, Joaquin Umana, William Cataldo, Mikhail Kozlov, Matthew Stone
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Patent number: 9815050Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).Type: GrantFiled: April 9, 2015Date of Patent: November 14, 2017Assignee: EMD Millipore CorporationInventors: David Yavorsky, John Amara, Joaquin Umana, William Cataldo, Mikhail Kozlov, Matthew Stone
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Publication number: 20150258540Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).Type: ApplicationFiled: April 9, 2015Publication date: September 17, 2015Inventors: David Yavorsky, John Amara, Joaquin Umana, William Cataldo, Mikhail Kozlov, Matthew Stone
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Patent number: 9067976Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.Type: GrantFiled: November 19, 2013Date of Patent: June 30, 2015Assignee: EMD Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Chen Wang, Nanying Bian, Brian Gagnon, Joaquin A. Umana, Dennis Aquino, Neil Soice, Andrew Lyddiatt
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Patent number: 9029517Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).Type: GrantFiled: July 27, 2011Date of Patent: May 12, 2015Assignee: EMD Millipore CorporationInventors: David Yavorsky, John Amara, Joaquin Umana, William Cataldo, Mikhail Kozlov, Matthew Stone
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Publication number: 20140073769Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.Type: ApplicationFiled: November 19, 2013Publication date: March 13, 2014Applicant: EMD Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Chen Wang, Nanying Bian, Brian Gagnon, Joaquin A. Umana, Dennis Aquino, Neil Soice, Lyddiatt Andrew
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Patent number: 8652330Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.Type: GrantFiled: March 2, 2010Date of Patent: February 18, 2014Assignee: EMD Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Chen Wang, Nanying Bian, Brian Gagnon, Joaquin A. Umana, Dennis Aquino, Neil Soice, Andrew Lyddiatt
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Publication number: 20130245139Abstract: The present invention provides novel compositions and methods for removal of protein aggregates from a sample in a flow-through mode.Type: ApplicationFiled: March 4, 2013Publication date: September 19, 2013Applicant: EMD Millpore CorporationInventors: Mikhail Kozlov, William Cataldo, Ajish Potty, Kevin Galipeau, James Hamzik, Joaquin Umana, Lars Peeck
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Publication number: 20130225701Abstract: The invention relates to improved methods of grafting polymer extenders onto porous substrates having diffusive pores, such as those used in protein separations, without filing the diffusive pores of the substrate, and restricting diffusion there through. By changing the grafting conditions and/or monomer composition(s) the resulting porous substrates having polymer extenders grafted thereto have increased protein binding capacity and resin selectivity, thereby enhancing the protein separation effectiveness of the substrate. The grafted polymer extenders provide the substrate with significant binding capacity at higher conductivity. The invention also relates to kits, and methods of using and grafting polymer extenders on porous resin substrates having diffusive pores.Type: ApplicationFiled: July 29, 2010Publication date: August 29, 2013Applicant: EMD MILLIPORE CORPORATIONInventors: Neil Soice, Joaquin Umana, Yu Zhang
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Publication number: 20120156757Abstract: The present invention relates, at least in part, to novel and improved chromatography matrices for separating or purifying immunogens, such as, for example, viruses and viral surface proteins, from one or more contaminants in a sample, where the matrix comprises a porous non-polysaccharide solid support comprising a negatively charged, multivalent ion exchange group directly attached to the solid support.Type: ApplicationFiled: December 13, 2011Publication date: June 21, 2012Applicant: Millipore CorporationInventors: Ganapathysubramanian Iyer, Joaquin A. Umana, Neil Soice
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Patent number: 8114611Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.Type: GrantFiled: November 1, 2010Date of Patent: February 14, 2012Assignee: Millipore CorporationInventors: Nanying Bian, John Charkoudian, Neil Soice, Joaquin Umana, Chen Wang
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Publication number: 20120029176Abstract: Adsorptive media for chromatography, particularly ion-exchange chromatography, derived from a shaped fiber. In certain embodiments, the functionalized shaped fiber presents a fibrillated or ridged structure which greatly increases the surface area of the fibers when compared to ordinary fibers. Also disclosed herein is a method to add surface pendant functional groups that provides cation-exchange or anion-exchange functionality to the high surface area fibers. This pendant functionality is useful for the ion-exchange chromatographic purification of biomolecules, such as monoclonal antibodies (mAbs).Type: ApplicationFiled: July 27, 2011Publication date: February 2, 2012Applicant: MILLIPORE CORPORATIONInventors: David Yavorsky, John Amara, Joaquin Umana, William Cataldo, Mikhail Kozlov, Matthew Stone
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Publication number: 20110105730Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.Type: ApplicationFiled: November 1, 2010Publication date: May 5, 2011Applicant: Millipore CorporationInventors: Nanying Bian, John Charkoudian, Neil Soice, Joaquin Umana, Chen Wang
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Patent number: 7846682Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.Type: GrantFiled: November 13, 2009Date of Patent: December 7, 2010Assignee: Millipore CorporationInventors: Nanying Bian, John Charkoudian, Neil Soice, Joaquin Umana, Chen Wang
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Patent number: 7833723Abstract: The invention provides methods of coupling protein ligands to a solid support. The invention also provides affinity chromatography matrices and methods of using affinity chromatography matrices to purify a target molecule.Type: GrantFiled: January 5, 2007Date of Patent: November 16, 2010Assignee: Millipore CorporationInventors: Nanying Bian, John Charkoudian, Neil Soice, Joaquin Umana, Chen Wang
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Publication number: 20100227015Abstract: The present invention relates to a method and apparatus for forming agarose or cored agarose beads. The process involves dissolving/gelation the agarose in a suitable liquid, mixing it with a hydrophobic liquid to form an emulsion and maintaining that emulsion at a temperature equal to or greater than the gelation point of the agarose, passing it through a static mixer to create agarose droplets and solidifying the agarose droplets in a second bath of hydrophobic liquid. The beads can then be washed and used or further processed to crosslink the agarose and/or add various functionalities on to the agarose. Another method for solidifying the agarose droplets is by using a heat exchanger to cool the stream continuously after it exits the static mixer. A similar process is used for the “cored” beads except cores, preferably in bead form, are introduced to the agarose before it enters the first hydrophobic liquid so that the agarose forms a coating on the cores.Type: ApplicationFiled: March 2, 2010Publication date: September 9, 2010Applicant: Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Nanying Bian, Brian Gagnon, Joaquin Umana, Neil P. Soice
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Publication number: 20100174051Abstract: The present invention relates to an asymmetric chromatography media suitable for separations applications, particularly as packed bed, fluidized bed or magnetized bed chromatography media. In certain embodiments, the asymmetric chromatography media comprises asymmetric particles, preferably beads, having at least two distinct, controlled pore size distributions. Preferably one of the distinct pore size distributions is in an internal region of the particle, and the other is in an external region or coating on the particle. These distinct pore size distributions can be modified with uniform or alternatively unique functional groups or mixtures of functional groups. The present invention allows for the control over pore size distribution within an asymmetric porous particle by providing a distinct internal region, preferably in the form of a bead, and a distinct external region, preferably in the form of a coating on the bead.Type: ApplicationFiled: March 2, 2010Publication date: July 8, 2010Applicant: Millipore CorporationInventors: Kwok-Shun Cheng, Senthilkumar Ramaswamy, Chen Wang, Nanying Bian, Brian Gagnon, Joaquin A. Umana, Dennis Aquino, Neil Soice, Lyddiatt Andrew