Abstract: Methods of producing bioconjugates of O-antigen polysaccharides covalently linked to a carrier protein using recombinant host cells are provided. The recombinant host cells used in the methods described herein encode a particular oligosaccharyl transferase enzyme depending on the O-antigen polysaccharide bioconjugate to be produced. The oligosaccharyl transferase enzymes can be PglB oligosaccharyl transferase or variants thereof. Also provided are compositions containing the bioconjugates, and methods of using the bioconjugates and compositions described herein to vaccinate a subject against extra-intestinal pathogenic E. coli. (ExPEC).

Abstract: The invention relates to a media combining device (M), in particular only one media combining device, preferably a common media combining device, for multiple fuel-cell systems (101, 102, 103), having the following: a container (MB) for mixing and/or examining flows of media, the following elements being arranged on said container: a respective line connection (M1) for each exhaust air line (12) for discharging exhaust air from a corresponding fuel-cell system (101, 102, 103), a respective line connection (MQ1) for each purge and/or drain line (L1) of a corresponding purge and/or drain system (Q1) of an anode system of a corresponding fuel-cell system (101, 102, 103), a line outlet (M2), in particular only one line outlet, for discharging flows of media out of the container (MB), and at least one fuel sensor(S) for detecting the fuel content in the container (MB).

Abstract: The invention relates to a method for controlling a drying process of a fuel cell system (100), in particular during shutdown of the fuel cell system (100), preferably in preparation for a start, in particular a cold start, of the fuel cell system (100), said method comprising the following steps: initiating a drying process of the fuel cell system (100), setting at least one operating parameter (i, ii, iii) in at least one functional system (1, 2, 3, 4) of the fuel cell system (100) to a constant level, monitoring at least one outlet temperature (TCathOut, TAnodOut) from a stack (101) of the fuel cell system (100) in at least one functional system (1, 2, 3, 4) of the fuel cell system (100), evaluating the at least one outlet temperature (TCathOut, TAnodOut), determining a termination time (tdryEnd) for ending the drying operation in accordance with the evaluation.

Abstract: The invention relates to a method for calibrating a fuel sensor (S) of a fuel cell system (100), wherein the method comprises the following steps: 1) opening a bypass valve (BV) in the bypass line (13) in order to operate the bypass line (13) in the open state; 2) closing shut-off valves (SV1, SV2) in the air supply line (11) and in the exhaust air line (12) in order to conduct all supply air from the air supply line (11) past the at least one fuel cell (101) and introduce it into the exhaust air line (12), 3) carrying out a zero-point calibration of the fuel sensor (S).

Abstract: The invention relates to a fuel cell system, having at least one fuel cell with an anode, a cathode, a membrane arranged between the anode and the cathode, a cathode inlet, a cathode outlet, an anode inlet, and an anode outlet. According to the invention, the fuel cell system is characterized in that the fuel cell system is designed to at least partly conduct water accumulating on the anode outlet at least partially to at least one humidification connection in an oxidant line connected to the cathode inlet so that an oxidant flow flowing to the cathode inlet is humidified.

Abstract: A fuel-cell system is proposed, comprising at least one fuel cell, an oxidant line, a hydrogen line, an exhaust-gas line, a control unit, and at least one electrically controllable valve, which is coupled to the control unit and is connected to one from among the oxidant line, the hydrogen line, and the exhaust-gas line. The fuel-cell system is distinguished in that the control unit is designed to activate the at least one valve in a pulsating or oscillating manner, at least during a first time interval, such that the at least one valve is prevented from remaining in a stationary state and seizing up by icing during the first time interval.

Abstract: The invention relates to a method for protecting components of a fuel cell system (1), the fuel cell system (1) having a fuel cell stack (101), an air path (10), an off-gas line (12), and a fuel line (20) with a recirculation circuit (50), the method comprising the steps of: monitoring the air path (10) for a fault; closing a first valve (61) which is situated in the air path (10) and closing a second valve (62) which is situated in the off-gas line (12); blocking a purge valve (41); reducing the pressure in the air path (10) upstream of the first valve (61); if further operation of the fuel cell system (1) is possible: increasing the pressure in the air path (10) upstream of the first valve (61); unblocking the purge valve (41); opening the first valve (61) and the second valve (62); further operating the fuel cell system (1).

Abstract: The invention relates to a method for setting an operating strategy for a fuel cell system (2) of a power generation device (1), in particular in the form of a vehicle, depending on an operating mode of the power generation device (1), having the steps of: a determination unit (3) determining at least one current operating parameter (P1) of the power generation device (1), the determination unit (3) determining at least one cumulative and/or predictive operating parameter (P2, P3, P4) of the power generation device (1), and a setting device (8) setting the operating strategy for the fuel cell system (2) on the basis of the at least one current operating parameter (P1) and the at least one cumulative and/or predictive operating parameter (P2, P3, P4) of the power generation device (1). The invention furthermore relates to a corresponding circuit arrangement (10), to a computer program (20) and to a storage means with a computer program (20) stored thereon.

Abstract: The invention relates to a fuel cell system (100), having: at least one fuel cell (101) and a cathode path (10) for providing an oxygen-containing reactant in the form of supply air (L1) to the at least one fuel cell (101), wherein the cathode path (10) has a supply air line (11) for providing the supply air (L1) to the at least one fuel cell (101) and an exhaust air line (12) for discharging exhaust air (L2) from the at least one fuel cell (101), and at least one heat exchanger (20) is provided between the supply air line (11) and the exhaust air line (12) of the cathode path (10) in order to transfer thermal energy from the supply air (L1) to the exhaust air (L2). For this purpose, the heat exchanger (20) is designed to transfer the heat to an exhaust air (L2) flow (M3) flowing through the heat exchanger (20) by means of the evaporation and condensation of product water (H20) and by means of multiple supply air (L1) flows (M1, M2) flowing through the heat exchanger (20).

Abstract: A computer-implemented method for a time-controlled delivery of updatable services to on-board systems of vehicles which use the services. The method includes analyzing the detected data to identify delivery time periods for the updatable services being optimal for each vehicle which uses the services, wherein the network usage of the backend server is optimally allotted to the specified time period on the basis of the availability of the data connection of the vehicles using the services to the backend server. A system for a time-controlled delivery of updatable services to on-board systems of vehicles that use the services is also disclosed.

Abstract: The invention relates to a heat exchanger system for operating a fuel cell stack, comprising: a first compressor and a second compressor for the cathode gas fed to the fuel cell stack, the second compressor being fluidically downstream of the first compressor; a turbine, which is mechanically coupled to the second compressor and against which the cathode gas discharged from the fuel cell stack flows; a first heat exchanger, which is thermally coupled to the fed cathode gas between the first compressor and the second compressor; a second heat exchanger, which is thermally coupled to the fed cathode gas downstream of the second compressor; a fourth heat exchanger, which is thermally coupled to the discharged cathode gas downstream of the turbine; wherein the fourth heat exchanger is thermally variably coupled to the first heat exchanger and to the second heat exchanger in order to control a heat exchange for cooling the first heat exchanger and the second heat exchanger.

Abstract: A computer-implemented method and system for usage-controlled service delivery to an onboard system of a vehicle are designed for delivering updatable services to the onboard system of the vehicle by way of a vehicle-external server according to data of the service usage behavior of a vehicle user of the updatable services.

Abstract: A method (100) for operating a gas bearing (1), wherein the gas bearing is formed by a rotor (11) and a stator (12), wherein when there is rotation against a stator (12) with a lift-off rotational speed nL the rotor (11) changes from mixed friction with the stator (12) into fluid friction with a medium (13) located between the stator (12) and the rotor (11), wherein the rotational speed of the rotor (11) is kept at or above an idling rotational speed nI, wherein—in response to a first information item (21), on the basis of which a change ?F is to be expected in the acceleration forces F acting on the gas bearing (1), a new value of a safety factor rN:=nI/nL between the idling rotational speed nI and the lift-off rotational speed nL is determined (110), and/or—in response to a second information item (31), on the basis of which a change ?nL is to be expected in the lift-off rotational speed nL, a new value nL,neu is determined for the lift-off rotational speed nL (120), wherein the idling rotational speed nI o

Abstract: The invention relates to a method for setting an operating strategy for a fuel cell system (2) of a power generation device (1), in particular in the form of a vehicle, depending on an operating mode of the power generation device (1), having the steps of: a determination unit (3) determining at least one current operating parameter (P1) of the power generation device (1), the determination unit (3) determining at least one cumulative and/or predictive operating parameter (P2, P3, P4) of the power generation device (1), and a setting device (8) setting the operating strategy for the fuel cell system (2) on the basis of the at least one current operating parameter (P1) and the at least one cumulative and/or predictive operating parameter (P2, P3, P4) of the power generation device (1).

Abstract: A device and method are disclosed for operating a fuel cell system having a fuel cell stack. Data are provided that map input variables of the fuel cell system and a position of a cell of the fuel cell stack on a voltage of the cell. A model is trained to map the input variables of the fuel cell system and the position of a cell of the fuel cell stack on a probability distribution for a prediction of a voltage of the cell. Instantaneous input variables of the fuel cell system are determined. A probability for the voltage of the cell or for a total voltage of the fuel cell stack is determined for a cell of the fuel cell stack based on the input variables using the model based on the probability distribution. A state of the fuel cell system is determined based on the probability.

Abstract: The invention relates to a method and to a system for operating a fuel cell system (22) and at least one sub-system (30) of the fuel cell system (22). According to the invention, these are arranged in a vehicle (10), wherein the energy for a drive train (12) of the vehicle (10) can be drawn both from the fuel cell system (22) and from an alternative energy store (26). The method comprises the following method steps: first, the number and duration of shut-down and/or stop phases of the vehicles (10) in a defined time interval in a first vehicle state (86) or in a second vehicle state (88) is determined based on vehicle state-specific learning functions (90, 112). Operating parameters of the fuel cell system (22) and of the at least one sub-system (30) of the fuel cell system (22) are then adjusted in dependence on the determined number and duration of shut-down and/or stop phases of the vehicle (10).

Abstract: The invention relates to a method and to a system for operating a fuel cell system (22) and at least one sub-system (30) of the fuel cell system (22). According to the invention, these are arranged in a vehicle (10), wherein the energy for a drive train (12) of the vehicle (10) can be drawn both from the fuel cell system (22) and from an alternative energy store (26). The method comprises the following method steps: first, the number and duration of shut-down and/or stop phases of the vehicles (10) in a defined time interval in a first vehicle state (86) or in a second vehicle state (88) is determined based on vehicle state-specific learning functions (90, 112). Operating parameters of the fuel cell system (22) and of the at least one sub-system (30) of the fuel cell system (22) are then adjusted in dependence on the determined number and duration of shut-down and/or stop phases of the vehicle (10).

Abstract: A forming station for a thermoform packaging machine comprising a forming tool upper part with a die lid and a tool lower part. The forming tool upper part may comprises a male die part holding plate that has at least one male die part fixed thereto and which is displaceable along at least one rail provided on or in the die lid. The forming station may comprise a male die part drive drivingly connected to the male die part holding plate for generating a stroke movement of the male die part holding plate relative to the die lid. A guide element may be provided on the male die part holding plate, the guide element being movable relative to the male die part holding plate between an engagement position for engagement with an associated rail and a retracted position in which the guide element is disengaged from the rail.

Abstract: A method (100) for operating a gas bearing (1), wherein the gas bearing is formed by a rotor (11) and a stator (12), wherein when there is rotation against a stator (12) with a lift-off rotational speed nL the rotor (11) changes from mixed friction with the stator (12) into fluid friction with a medium (13) located between the stator (12) and the rotor (11), wherein the rotational speed of the rotor (11) is kept at or above an idling rotational speed nI, wherein —in response to a first information item (21), on the basis of which a change ?F is to be expected in the acceleration forces F acting on the gas bearing (1), a new value of a safety factor rN:=nI/nL between the idling rotational speed nI and the lift-off rotational speed nL is determined (110), and/or —in response to a second information item (31), on the basis of which a change ?nL is to be expected in the lift-off rotational speed nL, a new value nL,neu is determined for the lift-off rotational speed nL (120), wherein the idling rotational speed nI

Abstract: In a method for the production of a rotary permanently excited electrical machine, a magnet body of magnetizable but not yet magnetized material is secured in or on a rotor body of a rotor such that the magnet body is arranged in a region of pole yet to be formed. An electrical conductor is arranged around the pole yet to be formed and the rotor body is fastened on a rotor shaft. The rotor shaft, including the rotor body with the magnet body and the electrical conductor, is mounted in a subsequent operating position relative to a stator. A pulse current is applied to the electrical conductor after mounting of the rotor shaft to thereby form the pole of the rotor as the magnet body is magnetized, and ends of the electrical conductor are electrically connected to one another after formation of the pole of the rotor.