Patents by Inventor Jochen Forster
Jochen Forster has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11866749Abstract: Described herein are recombinant microbial host cells comprising biosynthetic pathways and their use in producing oxidation products and downstream products, e.g., melatonin and related compounds, as well as enzyme variants, nucleic acids, vectors and methods useful for preparing and using such cells. In specific aspects, the present invention relates to monooxygenases, e.g., amino acid hydroxylases, with a modified cofactor-dependency, and to enzyme variants and microbial cells providing for an improved supply of cofactors.Type: GrantFiled: October 23, 2020Date of Patent: January 9, 2024Assignee: Danmarks Tekniske UniversitetInventors: Hao Luo, Jochen Förster
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Publication number: 20230323282Abstract: The present invention relates to yeast strains of the species Saccharomyces pastorianus with the useful characteristic of producing low levels of diacetyl during fermentation. Also provided are methods of producing a malt and/or cereal based beverage with these strains, as well as beverages produced by this method.Type: ApplicationFiled: June 29, 2021Publication date: October 12, 2023Applicant: Carlsberg A/SInventors: Klaus Lengeler, Michael Katz, Jochen Förster, Ross Fennessy, Claes Gjermansen, Anna Chailyan
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Publication number: 20230212487Abstract: The invention relates to yeast strains with useful characteristics, including not being capable of producing phenolic off-flavors and/or not capable of utilizing maltose or which has limited ability to utilize maltose. Also provided is methods of producing cereal based beverages without phenolic off-flavors and/or a low alcohol or a non-alcoholic malt and/or cereal based beverage, as well as beverages produced by these methods.Type: ApplicationFiled: August 28, 2020Publication date: July 6, 2023Applicant: Carlsberg A/SInventors: Marc Serra Colomer, Zoran Gojkovic, Jochen Förster, Natalia Y. Solodovnikova, Ross Fennessy
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Publication number: 20210079372Abstract: Described herein are recombinant microbial host cells comprising biosynthetic pathways and their use in producing oxidation products and downstream products, e.g., melatonin and related compounds, as well as enzyme variants, nucleic acids, vectors and methods useful for preparing and using such cells. In specific aspects, the present invention relates to monooxygenases, e.g., amino acid hydroxylases, with a modified cofactor-dependency, and to enzyme variants and microbial cells providing for an improved supply of cofactors.Type: ApplicationFiled: October 23, 2020Publication date: March 18, 2021Inventors: Hao Luo, Jochen Förster
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Patent number: 10851365Abstract: Described herein are recombinant microbial host cells comprising biosynthetic pathways and their use in producing oxidation products and downstream products, e.g., melatonin and related compounds, as well as enzyme variants, nucleic acids, vectors and methods useful for preparing and using such cells. In specific aspects, the present invention relates to monooxygenases, e.g., amino acid hydroxylases, with a modified cofactor-dependency, and to enzyme variants and microbial cells providing for an improved supply of cofactors.Type: GrantFiled: March 30, 2017Date of Patent: December 1, 2020Assignee: Danmarks Tekniske UniversitetInventors: Hao Luo, Jochen Förster
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Publication number: 20190119663Abstract: Described herein are recombinant microbial host cells comprising biosynthetic pathways and their use in producing oxidation products and downstream products, e.g., melatonin and related compounds, as well as enzyme variants, nucleic acids, vectors and methods useful for preparing and using such cells. In specific aspects, the present invention relates to monooxygenases, e.g., amino acid hydroxylases, with a modified cofactor-dependency, and to enzyme variants and microbial cells providing for an improved supply of cofactors.Type: ApplicationFiled: March 30, 2017Publication date: April 25, 2019Inventors: HAO LUO, JOCHEN FÖRSTER
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Patent number: 10077455Abstract: Cells and cell cultures are provided that have improved tolerance to 3-hydroxypropionic acid (3HP). Genetic modifications to provide a mutated or overexpressed SFA1 gene or other enhancement of 3HP detoxification via a glutathione-dependent dehydrogenase reaction, including medium supplementation with glutathione, may be combined with a 3HP producing metabolic pathway.Type: GrantFiled: June 12, 2014Date of Patent: September 18, 2018Assignee: TECHNICAL UNIVERSITY OF DENMARKInventors: Kanchana Rueksomtawin Kildegaard, Irina Borodina, Jochen Förster, Jens Nielsen
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Patent number: 10066245Abstract: A yeast cell having a reduced level of activity of NAD dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has at least one exogenous gene encoding NADP dependent GAPDH and/or has up-regulation of at least one endogenous gene expressing NADP dependent GAPDH, wherein combined expression of the enzymes NADP dependent GAPDH, PDC, ALD, ACS, ACC* and MCR in said host cell increases metabolic flux towards 3-HP via malonyl-CoA compared to an otherwise similar yeast cell lacking said genetic modification.Type: GrantFiled: June 12, 2014Date of Patent: September 4, 2018Assignee: TECHNICAL UNIVERSITY OF DENMARKInventors: Niels Bjerg Jensen, Irina Borodina, Yun Chen, Jerome Maury, Kanchana Rueksomtawin Kildegaard, Jochen Förster, Jens Nielsen
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Patent number: 9868966Abstract: A genetically modified Saccharomyces cerevisiae including an active fermentation pathway producing 3-HP expresses an exogenous gene expressing the aminotransferase YhxA from Bacillus cereus AH1272 catalyzing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde. The yeast may also express a 3-hydroxyisobutyrate dehydrogenase (HIBADH) and a 3-hydroxypropanoate dehydrogenase (3-HPDH) and aspartate 1-decarboxylase. Additionally the yeast may express pyruvate carboxylase and aspartate aminotransferase.Type: GrantFiled: October 10, 2013Date of Patent: January 16, 2018Assignee: TECHNICAL UNIVERSITY OF DENMARKInventors: Irina Borodina, Kanchana Rueksomtawin Kildegaard, Jochen Förster, Fredrik Öberg
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Patent number: 9834793Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.Type: GrantFiled: April 20, 2015Date of Patent: December 5, 2017Assignee: EVOLVA SAInventors: Michael Katz, Hans Peter Smits, Jochen Forster, Jens Bredal Nielsen
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Patent number: 9822374Abstract: A recombinant micro-organism such as Saccharomyces cerevisiae which produces and excretes into culture medium a stilbenoid metabolite product when grown under stilbenoid production conditions, which expresses in above native levels a ABC transporter which transports said stilbenoid out of said micro-organism cells to the culture medium. The genome of the Saccharomyces cerevisiae produces an auxotrophic phenotype which is compensated by a plasmid which also expresses one or more of said enzymes constituting said metabolic pathway producing said stilbenoid, an expression product of the plasmid is genetically modified to include a ubiquitination tag sequence. Expression of an enzyme participating in catabolism of phenylalanine by the Ehrlich pathway is optionally reduced compared to its native expression level.Type: GrantFiled: May 24, 2011Date of Patent: November 21, 2017Assignee: EVOLVA SAInventors: Michael Patrik Katz, Thomas Durhuus, Hans Peter Smits, Jochen Förster
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Patent number: 9725743Abstract: A genetically engineered micro-organism having an operative metabolic pathway producing cinnamoyl-CoA and producing pinosylvin therefrom by the action of a stilbene synthase is used for pinosylvin production. Said cinnamic acid may be formed from L-phenylalanine by a L-phenylalanine ammonia lyase (PAL) which is one accepting phenylalanine as a substrate and producing cinammic acid therefrom, preferably such that if the PAL also accepts tyrosine as a substrate and forms coumaric acid therefrom, the ratio Km(phenylalanine)/Km(tyrosine) for said PAL is less than 1:1 and if said micro-organism produces a cinammate-4 -hydroxylase enzyme (C4H), the ratio Kcat(PAL)/Kcat(C4H) is at least 2:1.Type: GrantFiled: January 2, 2013Date of Patent: August 8, 2017Assignee: Evolva SAInventors: Michael Katz, Jochen Forster, Helga David, Hans Peter Schmidt, Malin Sendelius, Sara Peterson Bjorn, Thomas Thomasen Durhuus
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Publication number: 20160230200Abstract: Recombinant microbial cells and methods for producing 5HTP, melatonin and related compounds using such cells are described. More specifically, the recombinant microbial cell may comprise exogenous genes encoding one or more of an L-tryptophan hydroxylase, a 5-hydroxy-L-tryptophan decarboxylyase, a serotonin acetyltransferase, an acetylserotonin O-methyltransferase; and means for providing tetrahydrobiopterin (THB), and can be further genetically modified to enrich one or more of tryptophan, S-adenosyl-L-methinonine and acetyl coenzyme A. Related sequences and vectors for use in preparing such recombinant microbial cells are also described.Type: ApplicationFiled: September 5, 2014Publication date: August 11, 2016Inventors: JIANGFENG ZHU, NIELS BJERG JENSEN, XIAO CHEN, JOCHEN FÖRSTER, IRINA BORODINA
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Patent number: 9404129Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.Type: GrantFiled: October 15, 2014Date of Patent: August 2, 2016Assignee: Evolva SAInventors: Michael Katz, Hans Peter Smits, Jochen Forster, Jens Bredel Nielsen
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Publication number: 20160138056Abstract: A yeast cell having a reduced level of activity of NAD dependent glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has at least one exogenous gene encoding NADP dependent GAPDH and/or has up-regulation of at least one endogenous gene expressing NADP dependent GAPDH, wherein combined expression of the enzymes NADP dependent GAPDH, PDC, ALD, ACS, ACC* and MCR in said host cell increases metabolic flux towards 3-HP via malonyl-CoA compared to an otherwise similar yeast cell lacking said genetic modification.Type: ApplicationFiled: June 12, 2014Publication date: May 19, 2016Applicant: TECHNICAL UNIVERSITY OF DENMARKInventors: Niels Bjerg JENSEN, Irina BORODINA, Yun CHEN, Jerome MAURY, Kanchana Rueksomtawin KILDEGAARD, Jochen FÖRSTER, Jens NIELSEN
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Publication number: 20160130613Abstract: Cells and cell cultures are provided that have improved tolerance to 3-hydroxypropionic acid (3HP). Genetic modifications to provide a mutated or overexpressed SFA1 gene or other enhancement of 3HP detoxification via a glutathione-dependent dehydrogenase reaction, including medium supplementation with glutathione, may be combined with a 3HP producing metabolic pathway.Type: ApplicationFiled: June 12, 2014Publication date: May 12, 2016Applicant: TECHNICAL UNIVERSITY OF DENMARKInventors: Kanchana Rueksomtawin KILDEGAARD, Irina BORODINA, Jochen FÖRSTER, Jens NIELSEN
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Publication number: 20150361455Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.Type: ApplicationFiled: April 20, 2015Publication date: December 17, 2015Inventors: Michael KATZ, Hans Peter SMITS, Jochen FORSTER, Jens Bredel NIELSEN
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Publication number: 20150267228Abstract: A genetically modified Saccharomyces cerevisiae including an active fermentation pathway producing 3-HP expresses an exogenous gene expressing the aminotransferase YhxA from Bacillus cereus AH1272 catalysing a transamination reaction between beta-alanine and pyruvate to produce malonate semialdehyde. The yeast may also express a 3-hydroxyisobutyrate dehydrogenase (HIBADH) and a 3-hydroxypropanoate dehydrogenase (3-HPDH) and aspartate 1-decarboxylase. Additionally the yeast may express pyruvate carboxylase and aspartate aminotransferase.Type: ApplicationFiled: October 10, 2013Publication date: September 24, 2015Applicant: TECHNICAL UNIVERSITY OF DENMARKInventors: Irina Borodina, Kanchana Rueksomtawin Kildegaard, Jochen Förster, Fredrik Öberg
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Publication number: 20150152444Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.Type: ApplicationFiled: October 15, 2014Publication date: June 4, 2015Inventors: Michael KATZ, Hans Peter SMITS, Jochen FORSTER, Jens Bredel NIELSEN
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Patent number: 9040269Abstract: A recombinant micro-organism producing resveratrol by a pathway in which phenylalanine ammonia lyase (PAL) produces trans-cinnamic acid from phenylalanine, cinnamate 4-hydroxylase (C4H) produces 4-coumaric acid from said trans-cinnamic acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA, or in which L-phenylalanine- or tyrosine-ammonia lyase (PAL/TAL) produces 4-coumaric acid, 4-coumarate-CoA ligase (4CL) produces 4-coumaroyl CoA from said 4-coumaric acid, and resveratrol synthase (VST) produces said resveratrol from said 4-coumaroyl CoA. The micro-organism may be a yeast, fungus or bacterium including Saccharomyces cerevisiae, E. coli, Lactococcus lactis, Aspergillus niger, or Aspergillus oryzae.Type: GrantFiled: April 17, 2008Date of Patent: May 26, 2015Assignee: Evola SAInventors: Michael Katz, Hans Peter Smits, Jochen Förster, Jens Bredal Nielsen