Patents by Inventor Jochen Schaub
Jochen Schaub has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230203449Abstract: The present invention provides a basal cell culture medium and a feed medium with novel amino acid ratios and/or iron choline citrate as iron carrier that result in improved performance of mammalian cell culture processes, such as CHO cultivation and protein production processes, in particular in increased product titer (e.g. of monoclonal antibodies). Also provided are methods for culturing mammalian cells and producing a protein of interest using said basal cell culture medium and optionally feed medium. The invention also provides for a medium platform that comprises (i) the basal cell culture medium and (ii) the feed medium.Type: ApplicationFiled: November 21, 2022Publication date: June 29, 2023Inventors: Christoph CLEMENS, Jochen SCHAUB, Marie LINK, Peter SCHORN, Torsten SCHULZ
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Publication number: 20230131164Abstract: The present invention relates to the identification of a genomic integration site for heterologous polynucleotides in Chinese Hamster Ovary (CHO) cells resulting in high RNA and/or protein production. More specifically it relates to CHO cells comprising at least one heterologous polynucleotide stably integrated into the S100A gene cluster of the CHO genome and to methods for the production of said CHO cells. Further, the invention relates to a method for the production of a protein of interest using said CHO cell and to the use of said CHO cell for producing a protein of interest at high yield. Integration within these specific target regions leads to reliable, stable and high yielding production of an RNA and/or protein of interest, encoded by the heterologous polynucleotide.Type: ApplicationFiled: December 6, 2022Publication date: April 27, 2023Applicant: Boehringer Ingelheim International GmbHInventors: Markus Mueller, Jochen Schaub, Christian Bernloehr, Jennifer Koenitzer
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Patent number: 11560549Abstract: The present invention relates to the identification of a genomic integration site for heterologous polynucleotides in Chinese Hamster Ovary (CHO) cells resulting in high RNA and/or protein production. More specifically it relates to CHO cells comprising at least one heterologous polynucleotide stably integrated into the S100A gene cluster of the CHO genome and to methods for the production of said CHO cells. Further, the invention relates to a method for the production of a protein of interest using said CHO cell and to the use of said CHO cell for producing a protein of interest at high yield. Integration within these specific target regions leads to reliable, stable and high yielding production of an RNA and/or protein of interest, encoded by the heterologous polynucleotide.Type: GrantFiled: August 10, 2018Date of Patent: January 24, 2023Assignee: Boehringer Ingelheim International GmbHInventors: Markus Mueller, Jochen Schaub, Christian Bernloehr, Jennifer Koenitzer
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Patent number: 11505784Abstract: The present invention provides a basal cell culture medium and a feed medium with novel amino acid ratios and/or iron choline citrate as iron carrier that result in improved performance of mammalian cell culture processes, such as CHO cultivation and protein production processes, in particular in increased product titer (e.g. of monoclonal antibodies). Also provided are methods for culturing mammalian cells and producing a protein of interest using said basal cell culture medium and optionally feed medium. The invention also provides for a medium platform that comprises (i) the basal cell culture medium and (ii) the feed medium.Type: GrantFiled: March 31, 2016Date of Patent: November 22, 2022Assignee: Boehringer Ingelheim International GmbHInventors: Christoph Clemens, Jochen Schaub, Marie Link, Peter Schorn, Torsten Schulz
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Publication number: 20200216815Abstract: The present invention relates to the identification of a genomic integration site for heterologous polynucleotides in Chinese Hamster Ovary (CHO) cells resulting in high RNA and/or protein production. More specifically it relates to CHO cells comprising at least one heterologous polynucleotide stably integrated into the 5100A gene cluster of the CHO genome and to methods for the production of said CHO cells. Further, the invention relates to a method for the production of a protein of interest using said CHO cell and to the use of said CHO cell for producing a protein of interest at high yield. Integration within these specific target regions leads to reliable, stable and high yielding production of an RNA and/or protein of interest, encoded by the heterologous polynucleotide.Type: ApplicationFiled: August 10, 2018Publication date: July 9, 2020Inventors: Markus Mueller, Jochen Schaub, Christian Bernloehr, Jennifer Koenitzer
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Publication number: 20180346881Abstract: The present invention provides a basal cell culture medium and a feed medium with novel amino acid ratios and/or iron choline citrate as iron carrier that result in improved performance of mammalian cell culture processes, such as CHO cultivation and protein production processes, in particular in increased product titer (e.g. of monoclonal antibodies). Also provided are methods for culturing mammalian cells and producing a protein of interest using said basal cell culture medium and optionally feed medium. The invention also provides for a medium platform that comprises (i) the basal cell culture medium and (ii) the feed medium.Type: ApplicationFiled: March 31, 2016Publication date: December 6, 2018Inventors: Christoph CLEMENS, Jochen SCHAUB, Marie LINK, Peter SCHORN, Torsten SCHULZ
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Patent number: 8969043Abstract: Biopharmaceutical process development with recombinant protein producing mammalian cells has realized a tremendous increase in both productivity and product yields in the past years. These achievements can be mainly attributed to the advancements in cell line development, media, and process optimization. Only recently, genome-scale technologies enable a system-level analysis to elucidate the complex biomolecular basis of protein production in mammalian cells promising an increased process understanding and the deduction of knowledge-based approaches for further process optimization. The present invention describes a method for a rational cell culturing process using such a knowledge-based approach.Type: GrantFiled: May 27, 2010Date of Patent: March 3, 2015Assignee: Boehringer Ingelheim International GmbHInventors: Jochen Schaub, Torsten Schulz
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Publication number: 20120190005Abstract: Biopharmaceutical process development with recombinant protein producing mammalian cells has realized a tremendous increase in both productivity and product yields in the past years. These achievements can be mainly attributed to the advancements in cell line development, media, and process optimization. Only recently, genome-scale technologies enable a system-level analysis to elucidate the complex biomolecular basis of protein production in mammalian cells promising an increased process understanding and the deduction of knowledge-based approaches for further process optimization. The present invention describes a method for a rational cell culturing process using such a knowledge-based approach.Type: ApplicationFiled: May 27, 2010Publication date: July 26, 2012Applicant: BOEHRINGER INGELHEIM INTERNATIONAL GMBHInventors: Jochen Schaub, Torsten Schulz
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Patent number: 7585617Abstract: The invention relates to methods and agents for thermal conditioning of a biological cell, methods and uses of said agents for releasing and insulating ingredients outside the biological cell, and for quantitatively and qualitatively detecting the released ingredient and to a method for sampling biological cells which consists in carrying out in one step the sampling said biological cells in a culture medium and in releasing cellular ingredients during said sampling. Sampling devices are also disclosed.Type: GrantFiled: February 26, 2005Date of Patent: September 8, 2009Assignee: Insilico Biotechnology GmbHInventors: Michael Dauner, Jochen Schaub
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Publication number: 20070298484Abstract: The invention relates to methods and agents for thermal conditioning of a biological cell, methods and uses of said agents for releasing and insulating ingredients outside the biological cell, and for quantitatively and qualitatively detecting the released ingredient and to a method for sampling biological cells which consists in carrying out in one step the sampling said biological cells in a culture medium and in releasing cellular ingredients during said sampling. Sampling devices are also disclosed.Type: ApplicationFiled: February 26, 2005Publication date: December 27, 2007Applicant: INSILICO BIOTECHNOLOGY GMBHInventors: Michael Dauner, Jochen Schaub