Abstract: Disclosed herein include methods, compositions, and kits suitable for use in the delivery of polyribonucleotides and circuits. There are provided, in some embodiments, RNA exporter proteins comprising an RNA-binding domain, a membrane-binding domain, and an interaction domain capable of nucleating self-assembly. Disclosed herein include polynucleotides encoding cargo RNA molecule(s). In some embodiments, a plurality of RNA exporter proteins are capable of self-assembling into lipid-enveloped nanoparticles (LNs) secreted from a sender cell in which the RNA exporter proteins are expressed, thereby generating a population of LNs comprising a fusogen and exported cargo RNA molecule(s).

Abstract: A method of making a fluid cooled assembly that incorporates a base that forms a partial enclosure defining an interior void space and having a top wall that has a top surface and that defines at least one opening through the top wall to the void space, the base further defining fluid entrance and exit ports into the void space, the top wall being made of material that can be friction stir welded. A lid having a size and shape substantially conformal to the opening, having a top surface and a bottom surface that defines a set of downwardly extending pins, and that is formed of a material that can be friction stir welded to the base is placed into the opening so that the lid top surface is flush with the top surface of the base top wall and friction welding the lid to the base.

Abstract: A method and apparatus for securing snow tire chains to tires. Two cables are secured to snow tire chains secured about at least one tire utilizing a securing mechanism. The two cables are secured to the securing mechanism utilizing a connecting mechanism integrated with the securing mechanism. The cables are connected to the snow tire chains by a tensioning mechanism integrated with the securing mechanism. The securing mechanism is centrally mounted along a side of the tire. The securing mechanism can be configured with a chamber for receiving the cables within the securing mechanism. The tension mechanism is generally centrally located within the securing mechanism. The cables can be attached to the snow tire chains utilizing an attachment mechanism.

Abstract: A method and apparatus for securing snow tire chains to tires. Two cables are secured to snow tire chains secured about at least one tire utilizing a securing mechanism. The two cables to secured to the securing mechanism utilizing a connecting mechanism integrated with said securing mechanism. The cables are connected to the snow tire chains by a tensioning mechanism integrated with the securing mechanism. The securing mechanism is centrally mounted along a side of said a tire. The securing mechanism can be configured with a chamber for receiving the cables within said securing mechanism. The tension mechanism is generally centrally located within said securing mechanism. The cables can be attached to the snow tire chains utilizing an attachment mechanism.