Patents by Inventor Joe W. Gray
Joe W. Gray has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 6180349Abstract: The present invention provides rapid and inexpensive methods for determining the copy number of a test locus of interest. The methods generally involve monitoring the formation of amplification product using real time amplification detection systems to quantify the amount of test locus and reference loci in a test subject and the amount of test locus and reference loci in at least one control subject. The methods can be used to interrogate the copy number of loci containing simple sequence repeats. Since such sequences are ubiquitous in eukaryotic genomes, the present methods have wide-ranging applicability. The methods of the present invention can be used as diagnostic and prognostic tools and in correlating abnormal copy number values for specific loci with disease and effectiveness of different treatment options.Type: GrantFiled: May 18, 1999Date of Patent: January 30, 2001Assignee: The Regents of the University of CaliforniaInventors: David G. Ginzinger, Tony E. Godfrey, Ronald H. Jensen, Joe W. Gray
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Patent number: 6159685Abstract: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread.Type: GrantFiled: July 30, 1997Date of Patent: December 12, 2000Assignee: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Joe W. Gray, Anne Kallioniemi, Olli-Pekka Kallioniemi, Frederic Waldman
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Patent number: 6132961Abstract: Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome.Type: GrantFiled: June 7, 1995Date of Patent: October 17, 2000Assignee: The Regents of the University of CaliforniaInventors: Joe W. Gray, Daniel Pinkel
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Patent number: 6110673Abstract: The present invention provides new probes for the detection of chromosomal alterations associated with cancer, particularly ovarian cancer. The probes bind selectively with target nucleic acid sequences at 3q26 and at 19q13.1-19q13.2.Type: GrantFiled: April 5, 1997Date of Patent: August 29, 2000Assignee: The Regents of the University of CaliforniaInventors: Laleh Shayesteh, Joe W. Gray
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Patent number: 6066453Abstract: The present invention relates to DNA sequences from regions of copy number change on chromosome 20. The sequences can be used in hybridization methods for the identification of chromosomal abnormalities associated with various diseases.Type: GrantFiled: August 8, 1997Date of Patent: May 23, 2000Assignee: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Donna G. Albertson, Joe W. Gray
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Patent number: 5976790Abstract: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread.Type: GrantFiled: November 27, 1995Date of Patent: November 2, 1999Assignee: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Joe W. Gray, Anne Kallioniemi, Olli-Pekka Kallioniemi, Frederick Waldman, Masaru Sakamoto
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Patent number: 5965362Abstract: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread.Type: GrantFiled: November 27, 1995Date of Patent: October 12, 1999Assignee: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Joe W. Gray, Anne Kallioniemi, Olli-Pekka Kallioniemi, Frederic Waldman, Masaru Sakamoto
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Patent number: 5888730Abstract: A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences.Type: GrantFiled: October 6, 1995Date of Patent: March 30, 1999Assignee: The Regents of the University of CaliforniaInventors: Joe W. Gray, Heinz-Ulrich Weier
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Patent number: 5856097Abstract: Disclosed are new methods comprising the use, of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread.Type: GrantFiled: November 27, 1995Date of Patent: January 5, 1999Assignee: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Joe W. Gray, Anne Kallioniemi, Olli-Pekka Kallioniemi, Frederic Waldman, Masaru Sakamoto
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Patent number: 5851769Abstract: The present invention relates generally to the DNA mapping and sequencing technologies. In particular, the present invention provides enhanced methods and compositions for the physical mapping and positional cloning of genomic DNA. The present invention also provides a useful analytical technique to directly map cloned DNA sequences onto individual stretched DNA molecules.Type: GrantFiled: September 27, 1995Date of Patent: December 22, 1998Assignee: The Regents of the University of CaliforniaInventors: Joe W. Gray, Heinz-Ulrich G. Weier
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Patent number: 5840482Abstract: A method for producing a Y chromosome specific probe selected from highly repeating sequences on that chromosome is described. There is little or no nonspecific binding to autosomal and X chromosomes, and a very large signal is provided. Inventive primers allowing the use of PCR for both sample amplification and probe production are described, as is their use in producing large DNA chromosome painting sequences.Type: GrantFiled: October 10, 1990Date of Patent: November 24, 1998Assignee: The Regents of the University of CaliforniaInventors: Joe W. Gray, Heinz-Ulrich Weier
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Patent number: 5830645Abstract: The present invention provides methods of determining relative copy number of target nucleic acids and precise mapping of chromosomal abnormalities associated with disease. The methods of the invention use target nucleic acids immobilized on a solid surface, to which a sample comprising two sets of differentially labeled nucleic acids are hybridized. The hybridization of the labeled nucleic acids to the solid surface is then detected using standard techniques.Type: GrantFiled: December 9, 1994Date of Patent: November 3, 1998Assignees: The Regents of the University of California, The Medical Research CouncilInventors: Daniel Pinkel, Donna Albertson, Joe W. Gray
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Patent number: 5801021Abstract: The present invention relates to in situ hybridization methods for the identification of new chromosomal abnormalities associated with various diseases. In particular, it provides probes which are specific to a region of amplification in chromosome 20.Type: GrantFiled: October 20, 1995Date of Patent: September 1, 1998Assignee: The Regents of the University of CaliforniaInventors: Joe W. Gray, Colin Collins, Daniel Pinkel, Olli-Pekka Kallioniemi, Minna M. Tanner
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Patent number: 5756696Abstract: Methods and compositions for staining based upon nucleic acid sequence that employ nucleic acid probes are provided. Said methods produce staining patterns that can be tailored for specific cytogenetic analyses. Said probes are appropriate for in situ hybridization and stain both interphase and metaphase chromosomal material with reliable signals. The nucleic acid probes are typically of a complexity greater than 50 kb, the complexity depending upon the cytogenetic application. Methods are provided to disable the hybridization capacity of shared, high copy repetitive sequences and/or remove such sequences to provide for useful contrast. Still further methods are provided to produce chromosome-specific staining reagents which are made specific to the targeted chromosomal material, which can be one or more whole chromosomes, one or more regions on one or more chromosomes, subsets of chromosomes and/or the entire genome.Type: GrantFiled: December 23, 1994Date of Patent: May 26, 1998Assignee: Regents Of The University Of CaliforniaInventors: Joe W. Gray, Daniel Pinkel
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Patent number: 5721098Abstract: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread.Type: GrantFiled: June 6, 1995Date of Patent: February 24, 1998Assignee: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Joe W. Gray, Anne Kallioniemi, Olli-Pekka Kallioniemi, Frederic Waldman
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Patent number: 5670314Abstract: The present invention relates to compositions and methods for detecting chromosome abnormalities correlated with lung cancer. The method contacting a nucleic acid sample from a patient with a probe which binds selectively to a target polynucleotide sequence correlated with lung cancer.Type: GrantFiled: February 22, 1994Date of Patent: September 23, 1997Assignee: Regents of the University of CaliforniaInventors: Michael F. Christman, Joe W. Gray, Nikki A. Levin, Pius Brzoska, Haruhiko Nakamura
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Patent number: 5665549Abstract: Disclosed are new methods comprising the use of in situ hybridization to detect abnormal nucleic acid sequence copy numbers in one or more genomes wherein repetitive sequences that bind to multiple loci in a reference chromosome spread are either substantially removed and/or their hybridization signals suppressed. The invention termed Comparative Genomic Hybridization (CGH) provides for methods of determining the relative number of copies of nucleic acid sequences in one or more subject genomes or portions thereof (for example, a tumor cell) as a function of the location of those sequences in a reference genome (for example, a normal human genome). The intensity(ies) of the signals from each labeled subject nucleic acid and/or the differences in the ratios between different signals from the labeled subject nucleic acid sequences are compared to determine the relative copy numbers of the nucleic acid sequences in the one or more subject genomes as a function of position along the reference chromosome spread.Type: GrantFiled: June 6, 1995Date of Patent: September 9, 1997Assignee: The Regents of the University of CaliforniaInventors: Daniel Pinkel, Joe W. Gray, Anne Kallioniemi, Olli-Pekka Kallioniemi, Frederic Waldman
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Patent number: 5633365Abstract: The present invention relates to in situ hybridization methods for the identification of new chromosomal abnormalities associated with various diseases. In particular, it provides probes which are specific to a region of amplification in chromosome 20.Type: GrantFiled: April 28, 1995Date of Patent: May 27, 1997Assignee: The Regents of the University of CaliforniaInventors: Trond Stokke, Daniel Pinkel, Joe W. Gray
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Patent number: 5472842Abstract: The present invention relates to in situ hybridization methods for the identification of new chromosomal abnormalities associated with various diseases. In particular, it provides probes which are specific to a region of amplification in chromosome 20.Type: GrantFiled: October 6, 1993Date of Patent: December 5, 1995Assignee: The Regents of the University of CaliforniaInventors: Trond Stokke, Daniel Pinkel, Joe W. Gray
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Patent number: 5447841Abstract: Methods and compositions for chromosome-specific staining are provided. Compositions comprise heterogenous mixtures of labeled nucleic acid fragments having substantially complementary base sequences to unique sequence regions of the chromosomal DNA for which their associated staining reagent is specific. Methods include methods for making the chromosome-specific staining compositions of the invention, and methods for applying the staining compositions to chromosomes.Type: GrantFiled: December 14, 1990Date of Patent: September 5, 1995Assignee: The Regents Of The Univ. Of CaliforniaInventors: Joe W. Gray, Daniel Pinkel