Patents by Inventor Joel A. Farkas

Joel A. Farkas has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Restriction/modification polypeptides, polynucleotides, and methods
    Patent number: 8962333
    Abstract: The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.
    Type: Grant
    Filed: April 4, 2012
    Date of Patent: February 24, 2015
    Assignee: University of Georgia Research Foundation, Inc.
    Inventors: Janet Westpheling, DaeHwan Chung, Jennifer Huddleston, Joel A. Farkas
  • RESTRICTION/MODIFICATION POLYPEPTIDES, POLYNUCLEOTIDES, AND METHODS
    Publication number: 20120252072
    Abstract: The present invention relates to the discovery of a novel restriction/modification system in Caldicellulosiruptor bescii. The discovered restriction enzyme is a HaeIII-like restriction enzyme that possesses a thermophilic activity profile. The restriction/modification system also includes a methyltransferase, M.CbeI, that methylates at least one cytosine residue in the CbeI recognition sequence to m4C. Thus, the invention provides, in various aspects, isolated CbeI or M.CbeI polypeptides, or biologically active fragments thereof; isolated polynucleotides that encode the CbeI or M.CbeI polypeptides or biologically active fragments thereof, including expression vectors that include such polynucleotide sequences; methods of digesting DNA using a CbeI polypeptide; methods of treating a DNA molecule using a M.CbeI polypeptide; and methods of transforming a Caldicellulosiruptor cell.
    Type: Application
    Filed: April 4, 2012
    Publication date: October 4, 2012
    Inventors: JANET WESTPHELING, DaeHwan Chung, Jennifer Huddleston, Joel A. Farkas