Patents by Inventor Joel A. Jessee

Joel A. Jessee has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 9765359
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Grant
    Filed: January 12, 2016
    Date of Patent: September 19, 2017
    Assignee: Molecular Transfer, Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Publication number: 20170253888
    Abstract: Disclosed herein are transfection complexes comprising at least one cell surface ligand; at least one helper lipid component; and a transfection enhancer. Also disclosed are pharmaceutical compositions comprising the disclosed transfection complexes, and a pharmaceutically acceptable carrier. Further, disclosed are methods of transfecting a cell, the method comprising the steps of: obtaining a transfection complex as disclosed; and contacting a cell with the transfection complex.
    Type: Application
    Filed: March 1, 2017
    Publication date: September 7, 2017
    Inventors: Gulilat GEBEYEHU, Joel JESSEE
  • Publication number: 20170029448
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes. The present invention is in the field of molecular biology, and more particularly in the field of transfection, which utilizes new compounds and methods for the introduction of nucleic acids into eukaryotic cells.
    Type: Application
    Filed: October 28, 2013
    Publication date: February 2, 2017
    Applicant: MOLECULAR TRANSFER, INC.
    Inventors: Gulilat GEBEYEHU, Joel JESSEE
  • Publication number: 20160130607
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Application
    Filed: January 12, 2016
    Publication date: May 12, 2016
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Patent number: 9259475
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Grant
    Filed: October 16, 2013
    Date of Patent: February 16, 2016
    Assignee: Molecular Transfer, Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Publication number: 20160017391
    Abstract: The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
    Type: Application
    Filed: June 19, 2015
    Publication date: January 21, 2016
    Inventors: Wu-Bo LI, Joel Jessee, Christian Gruber
  • Publication number: 20140295502
    Abstract: The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
    Type: Application
    Filed: May 13, 2014
    Publication date: October 2, 2014
    Inventors: Wu-Bo LI, Joel Jessee, Christian Gruber
  • Publication number: 20140213637
    Abstract: New cationic lipids are provided that are useful for delivering macromolecules, such as nucleic acids, into eukaryotic cells. The lipids can be used alone, in combination with other lipids and/or in combination with other transfection enhancing reagents to prepare transfection complexes.
    Type: Application
    Filed: October 16, 2013
    Publication date: July 31, 2014
    Applicant: Molecular Transfer Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Patent number: 8759499
    Abstract: Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.
    Type: Grant
    Filed: March 28, 2011
    Date of Patent: June 24, 2014
    Assignee: Molecular Transfer, Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Publication number: 20120122760
    Abstract: Cationic lipid compositions are provided that are useful for efficient delivery of macromolecules, such as nucleic acids, into a wide variety of eukaryotic cell types. Methods for using the compositions also are provided.
    Type: Application
    Filed: October 11, 2011
    Publication date: May 17, 2012
    Inventors: Gulilat Gebeyehu, Taysir M. Jaounl, Joel Jessee
  • Publication number: 20120107879
    Abstract: The present invention is directed to methods for the production and isolation of nucleic acid molecules. In particular, the invention concerns isolation of mRNA molecules and the production and isolation of nucleic acid molecules (e.g., cDNA molecules or libraries), which may be single- or double-stranded. Additionally, the invention concerns selection and isolation of particular nucleic acid molecules of interest from a sample which may contain a population of molecules. Specifically, the invention concerns affinity-labeled primer-adapter molecules which allow improved isolation and production of such nucleic acid molecules, increasing both product recovery and speed of isolation.
    Type: Application
    Filed: August 29, 2011
    Publication date: May 3, 2012
    Applicant: Life Technologies Corporation
    Inventors: Christian E. GRUBER, Joel A. Jessee
  • Publication number: 20120071354
    Abstract: The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.
    Type: Application
    Filed: August 22, 2011
    Publication date: March 22, 2012
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Wu-Bo Li, Paul E. Nisson, Joel Jessee
  • Publication number: 20120052574
    Abstract: Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.
    Type: Application
    Filed: March 28, 2011
    Publication date: March 1, 2012
    Applicant: Molecular Transfer, Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Patent number: 8058068
    Abstract: The present invention provides compositions useful for transfecting eukaryotic cells comprising nucleic acid complexes with peptides, wherein the peptide is optionally covalently coupled to a nucleic acid-binding group, and cationic lipids or dendrimers as transfection agents. The invention also provides transfection compositions in which a peptide is covalently linked to the transfection agent (lipid, cationic lipid or dendrimer). Inclusion of peptides or modified-peptides in transfection compositions or covalent attachment of peptides to transfection agents results in enhanced transfection efficiency. Methods for the preparation of transfection compositions and methods of using these transfection compositions as intracellular delivery agents and extracellular targeting agents are also disclosed.
    Type: Grant
    Filed: July 23, 2002
    Date of Patent: November 15, 2011
    Assignee: Life Technologies Corporation
    Inventors: Pamela Hawley-Nelson, Jianqing Lan, PoJen Shih, Joel A. Jessee, Kevin P. Schifferli, Gulilat Gebeyehu, Valentina C. Ciccarone, Krista L. Evans
  • Patent number: 8034977
    Abstract: Cationic lipid compositions are provided that are useful for efficient delivery of macromolecules, such as nucleic acids, into a wide variety of eukaryotic cell types. Methods for using the compositions also are provided.
    Type: Grant
    Filed: April 27, 2009
    Date of Patent: October 11, 2011
    Assignee: Molecular Transfer, Inc.
    Inventors: Gulilat Gebeyehu, Taysir M. Jaouni, Joel Jessee
  • Publication number: 20110200624
    Abstract: Novel peptides derived from antibody complementarity determining regions (CDRs) that enhance delivery of macromolecules into cells, particularly when used in combination with cationic lipids, are provided. The peptides can be combined with cationic lipids, and compositions of cationic lipids associated with enhancer elements, to provide reagents that can complex with macromolecules such as nucleic acids, proteins and peptides and permit introduction of these macromolecules into a variety of cells and tissues in vitro or in vivo with greatly enhanced efficiency compared to other lipid-based reagents. Methods for delivering macromolecules into target cells and tissues using the lipids and enhancer elements are provided.
    Type: Application
    Filed: July 31, 2009
    Publication date: August 18, 2011
    Applicant: MOLECULAR TRANSFER, INC.
    Inventor: Joel A. Jessee
  • Publication number: 20110151521
    Abstract: The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
    Type: Application
    Filed: January 31, 2011
    Publication date: June 23, 2011
    Applicant: Life Technologies Corporation
    Inventors: Wu Bo Li, Joel A. Jessee, Christian E. GRUBER
  • Patent number: 7915230
    Abstract: Compositions and methods for improved delivery of macromolecules into eukaryotic cells are provided. Fusogenic peptides from fusion proteins of non-enveloped viruses enhance the efficiency of transfection of eukaryotic cells mediated by transfection agents such as cationic lipids, polycationic polymers such as PEI and dendrimers. These fusogenic peptides are used as part of a transfection complex that efficiently delivers a macromolecule, for example, a nucleic acid, into a eukaryotic cell. Novel cationic lipids and compositions of cationic lipids also are provided that may be used for the introduction of macromolecules such as nucleic acids, proteins and peptides into a variety of cells and tissues. The lipids can be used alone, in combination with other lipids and/or in combination with fusogenic peptides to prepare transfection complexes.
    Type: Grant
    Filed: May 17, 2006
    Date of Patent: March 29, 2011
    Assignee: Molecular Transfer, Inc.
    Inventors: Joel Jessee, Gulilat Gebeyehu
  • Publication number: 20100317063
    Abstract: The present invention generally relates to methods of making cDNA molecules and cDNA libraries. The invention also relates to cDNA molecules and cDNA libraries produced according to these methods, as well as to vectors and host cells containing such cDNA molecules and libraries. The invention also relates to kits for making the cDNA molecules and libraries of the invention.
    Type: Application
    Filed: November 21, 2009
    Publication date: December 16, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Wu Bo LI, Joel A. Jessee, Christian E. Gruber
  • Publication number: 20100279898
    Abstract: The present invention relates generally to methods for producing normalized nucleic acid libraries in which each member of the library can be isolated with approximately equivalent probability. In particular, the present methods comprise subtractive hybridization of a nucleic acid library with haptenylated (e.g., biotinylated, avidinated or streptavidinated) nucleic acid molecules that are complementary to one or more of the nucleic acid molecules of the library, such that the variation in the abundances of the individual nucleic acid molecules in the library is reduced. The invention also relates to production of normalized nucleic acid libraries (particularly cDNA libraries) in which contaminating nucleic acid molecules have been reduced or eliminated, and to normalized nucleic acid libraries produced by such methods.
    Type: Application
    Filed: April 19, 2010
    Publication date: November 4, 2010
    Applicant: LIFE TECHNOLOGIES CORPORATION
    Inventors: Wu-Bo Li, Paul E. Nisson, Joel JESSEE