Abstract: Methods are provided for producing recombinant proteins by utilizing expression vectors carrying nucleic acids encoding the proteins, and secretory signal sequences to direct the secretion of the proteins to the periplasm or extracellular medium. Expression vectors which encode a fusion protein comprising a carrier protein and the protein are also provided, as are host cells transformed with the expression vectors.

Abstract: A method of producing a protein or polypeptide, in which an expression vector that encodes a fusion protein comprising the protein or polypeptide peptide located carboxy-terminally to YebF, or a biologically active variant or portion thereof, is expressed in a suitable bacterial cell, to make the fusion protein. The method may further comprise the step of purifying the secreted fusion protein from the growth medium. The fusion protein may further comprise a peptide tag, or a protein cleavage site to separate the protein or polypeptide from the YebF portion, or the tag. Expression of a fusion protein into the culture medium provides a starting material for purification, in which the secreted fusion protein is relatively pure.

Abstract: Methods and apparatuses for advanced, multiple-projection, dual-energy X-ray absorptiometry scanning systems include combinations of a conical collimator; a high-resolution two-dimensional detector; a portable, power-capped, variable-exposure-time power supply; an exposure-time control element; calibration monitoring; a three-dimensional anti-scatter-grid; and a gantry-gantry base assembly that permits up to seven projection angles for overlapping beams. Such systems are capable of high precision bone structure measurements that can support three dimensional bone modeling and derivations of bone strength, risk of injury, and efficacy of countermeasures among other properties.

Abstract: A static dissipative electrical cable which eliminates the build-up of triboelectric charging by having a carbon loaded outer jacket which is a semiconductor for draining off triboelectric charges. In one embodiment, the jacket material is a 1 megohm/ft semiconductive extrudable Teflon polyfluorinated alkoxy (PFA) resin static-dissipative polymer of low carbon-black loading (3-4% by weight) having very stable electrical/mechanical properties against temperature excursions, thermal/environmental shock and aging. The cable is designed with a double-clad aluminum/mylar foil shield that allows electrical commoning between inner and outer clads of the foil and control of the interface between semi-conductive static-dissipative jacket and the foil shield to yield a jacket-to-ground wire resistance value between 100 kilo-ohms and 50 megohms.