Patents by Inventor Joel Credle

Joel Credle has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20250171834
    Abstract: In one aspect, compositions are provided for the simultaneous in situ quantification and localization of RNA sequences with subcellular precision are utilized in the methods. In particular, specific hybridization of at least one probe set in a sample is followed by in situ ligation, which locks specifically circularized probe set around an RNA target sequence. Rolling circle amplification followed by fluorescently labeled detector probe hybridization, enables simultaneous in situ quantification and localization of RNA sequences with subcellular precision.
    Type: Application
    Filed: June 7, 2023
    Publication date: May 29, 2025
    Inventors: Harry Larman, Joel Credle
  • Publication number: 20240401156
    Abstract: The present invention relates to the field of ribonucleic acid (RNA). More specifically, the present invention provides compositions and methods for highly multiplexed detection of pathogen-associated RNA. In a specific embodiment, a method for forming a target ribonucleic acid (RNA) proxy in a sample comprises the steps of (a) contacting a sample with one or more multi-partite probes that hybridize to a target RNA, wherein the one or more multi-partite probes comprise (i) a target capture probe, (ii) a 3?acceptor probe and (iii) a 5? phosphorylated donor probe; (b) incubating the sample of step (a) under conditions that allow hybridization of the one or more multi-partite probes to target RNA present in the sample; (c) immobilizing the target capture probes on a solid support; (d) washing away unbound multi-partite probes; and € ligating the acceptor probes and donor probes to form a target RNA proxy.
    Type: Application
    Filed: October 8, 2020
    Publication date: December 5, 2024
    Inventors: Harry B. Larman, Joel Credle
  • Publication number: 20230323428
    Abstract: The present disclosure relates to compositions and methods of RNA analysis. In particular, the present disclosure provides a method of RNA analysis that includes obtaining a sample, applying one or more multi-partite probes to the sample, where each of the one or more multi-partite probes includes at least two sub-probes, annealing at least one of the applied one or more multi-partite probes to at least one target nucleic acid within the sample, and ligating the at least two sub-probes associated with the at least one annealed multi-partite probe to create a target nucleic acid proxy that can be detected.
    Type: Application
    Filed: June 14, 2023
    Publication date: October 12, 2023
    Applicant: The Johns Hopkins University
    Inventors: Harry Benjamin Larman, Joel Credle
  • Publication number: 20180208967
    Abstract: The present disclosure relates to compositions and methods of RNA analysis. In particular, the present disclosure provides a method of RNA analysis that includes obtaining a sample, applying one or more multi-partite probes to the sample, where each of the one or more multi-partite probes includes at least two sub-probes, annealing at least one of the applied one or more multi-partite probes to at least one target nucleic acid within the sample, and ligating the at least two sub-probes associated with the at least one annealed multi-partite probe to create a target nucleic acid proxy that can be detected.
    Type: Application
    Filed: July 22, 2016
    Publication date: July 26, 2018
    Inventors: Harry Benjamin Larman, Joel Credle
  • Patent number: 9562258
    Abstract: A method for detecting the presence of a target nucleotide sequence in a sample of DNA is described herein in which a test sample comprising single stranded DNA is exposed to a DNA probe and a nicking endonuclease under conditions that would permit sequence-specific hybridization of the probe to a complementary target sequence. The probe comprises a sequence complementary to the target sequence to be detected and this sequence also includes a recognition sequence for the nicking endonuclease. If the sample contains the target sequence, the probe hybridizes to the target and is cleaved by the nicking endonuclease, which leaves the target intact. Observing the presence of probe cleaved by the nicking endonuclease indicates the presence of the target nucleotide sequence in the sample of DNA.
    Type: Grant
    Filed: February 3, 2014
    Date of Patent: February 7, 2017
    Assignee: Georgetown University
    Inventors: Mark Danielsen, Joel Credle, Eugene A. Davidson, Kenneth L. Dretchen
  • Publication number: 20140256577
    Abstract: A method for detecting the presence of a target nucleotide sequence in a sample of DNA is described herein in which a test sample comprising single stranded DNA is exposed to a DNA probe and a nicking endonuclease under conditions that would permit sequence-specific hybridization of the probe to a complementary target sequence. The probe comprises a sequence complementary to the target sequence to be detected and this sequence also includes a recognition sequence for the nicking endonuclease. If the sample contains the target sequence, the probe hybridizes to the target and is cleaved by the nicking endonuclease, which leaves the target intact. Observing the presence of probe cleaved by the nicking endonuclease indicates the presence of the target nucleotide sequence in the sample of DNA.
    Type: Application
    Filed: February 3, 2014
    Publication date: September 11, 2014
    Applicant: Georgetown University
    Inventors: Mark DANIELSEN, Joel Credle, Eugene A. Davidson, Kenneth L. Dretchen
  • Publication number: 20120065088
    Abstract: A method for detecting the presence of a target nucleotide sequence in a sample of DNA is described herein in which a test sample comprising single stranded DNA is exposed to a DNA probe and a nicking endonuclease under conditions that would permit sequence-specific hybridization of the probe to a complementary target sequence. The probe comprises a sequence complementary to the target sequence to be detected and this sequence also includes a recognition sequence for the nicking endonuclease. If the sample contains the target sequence, the probe hybridizes to the target and is cleaved by the nicking endonuclease, which leaves the target intact. Observing the presence of probe cleaved by the nicking endonuclease indicates the presence of the target nucleotide sequence in the sample of DNA.
    Type: Application
    Filed: February 23, 2010
    Publication date: March 15, 2012
    Applicant: Georgetown University
    Inventors: Mark Danielsen, Joel Credle, Eugene A. Davidson, Kenneth L. Dretchen