Abstract: An engineered extracellular vesicle (EV) for delivering a bioactive cargo, the engineered EV comprising an endosomal escape enhancer and a cargo. Plasmids, polynucleotide sequences, cells, methods of making and analysis, pharmaceutical compositions and uses thereof.

Abstract: The present invention relates to engineered extracellular vesicles (EVs) as a therapeutic modality for the treatment of various severe diseases. More specifically, the invention relates to a novel approach to manufacturing engineered EVs which extends their half-life and alters their biodistribution, resulting in a novel therapeutic modality able to carry various types of drugs suitable for application in not only genetic diseases but more broadly across essentially all therapeutic areas.

Abstract: The present invention pertains to an inventive release mechanism for extracellular vesicle (EV)-mediated intracellular and intramembrane delivery of therapeutic polypeptides. More specifically, the invention relates to EVs comprising polypeptide constructs which comprise a therapeutic polypeptide releasably attached to an exosomal polypeptide. Furthermore, the present invention pertains to manufacturing methods, pharmaceutical compositions, medical uses and applications, and various other embodiments related to the inventive EVs.

Abstract: The present invention pertains to inter alia methods for purifying extracellular vesicles including exposing a sample comprising at least one extracellular vesicle to ultrafiltration; and exposing the sample following the ultrafiltration in step (i) to size exclusion liquid chromatography.

Abstract: The present invention pertains to inter alia methods for purifying extracellular vesicles including exposing a sample comprising at least one extracellular vesicle to ultrafiltration; and exposing the sample following the ultrafiltration in step (i) to size exclusion liquid chromatography.

Abstract: A gripper unit is provided, comprising a housing and a vacuum distribution unit connected to the housing such that linear motion of the vacuum distribution unit is allowed in a first direction towards and away from the housing.

Abstract: The present invention pertains to an inventive release mechanism for extracellular vesicle (EV)-mediated intracellular and intramembrane delivery of therapeutic polypeptides. More specifically, the invention relates to EVs comprising polypeptide constructs which comprise a therapeutic polypeptide releasably attached to an exosomal polypeptide. Furthermore, the present invention pertains to manufacturing methods, pharmaceutical compositions, medical uses and applications, and various other embodiments related to the inventive EVs.

Abstract: The present invention pertains to inter alia therapeutic delivery vesicles, for instance exosomes or microvesicles, comprising polypeptide constructs, methods for producing said therapeutic delivery vesicles, pharmaceutical compositions and medical uses thereof. The therapeutic polypeptide constructs comprised in the extracellular delivery vesicles enable sequestering target molecules of interest, to treat e.g. neuro-inflammatory diseases and cancer.

Abstract: The present invention pertains to extracellular vesicle (EV) therapeutics, wherein the EVs comprise nucleic acid (NA)-based therapeutics such as mRNAs, circular RNAs, miRNAs, shRNAs, circular RNA and/or DNA molecules. The NA therapeutics are loaded into EVs using inventive engineering protein and NA engineering strategies to enhance loading into EVs and to facilitate release of the NA cargo molecules inside target cells.

Abstract: The present invention relates to extracellular vesicle (EV)-mediated delivery of protein-based therapeutics. More specifically, the invention relates to delivery of complex polypeptide-based agents which typically bind to target proteins extracellularly, intracellularly, or in the cell membrane.

Abstract: The present disclosure is related to a device for aligning a number of packages including at least one bottom belt. In some embodiments, the at least one bottom belt may include a first end portion for receiving packages and a second end portion for exiting packages. In some embodiments, said at least one bottom belt may include a laterally declined portion facing a support surface arranged along a side of the at least one bottom belt, whereby in use, packages conveyed by the at least one bottom belt move laterally towards the support surface for alignment thereto due to the laterally declined portion and by means of gravity.

Abstract: The present invention relates to engineered cells that enable exosome-mediated delivery of therapeutic cargoes, in particular protein therapeutics and RNA therapeutics. The present invention also relates to inventive polynucleotides, polypeptides and pharmaceutical compositions.

Abstract: The present invention pertains to inter alia therapeutic delivery vesicles, for instance exosomes or microvesicles, comprising polypeptide constructs, methods for producing said therapeutic delivery vesicles, pharmaceutical compositions and medical uses thereof. The therapeutic polypeptide constructs comprised in the extracellular delivery vesicles enable sequestering target molecules of interest, to treat e.g. neuro-inflammatory diseases and cancer.

Abstract: The present invention pertains to improved methods for loading of extracellular vesicles (EVs), such as exosomes, with various types of proteins of interest. More specifically, the invention relates to loading of EVs using fusion polypeptide constructs, as well as i.a. fusion constructs per se and EVs carrying such fusion polypeptides. The design of the fusion polypeptides is key to enable both efficient surface-display and internal loading into EVs of proteins of interest.

Abstract: The present invention is related to a device for aligning a number of packages comprising, at least one bottom belt for receiving packages at a first end portion and exiting packages at a second end portion, wherein said bottom belt comprises a laterally declined portion facing a support surface arranged along a side of one of the bottom belts, whereby in use packages conveyed by the bottom belts move laterally towards the support surface for alignment thereto due to the declined portion and by means of gravity.

Abstract: The present invention pertains to an inventive release mechanism for extracellular vesicle (EV)-mediated intracellular and intramembrane delivery of therapeutic polypeptides. More specifically, the invention relates to EVs comprising polypeptide constructs which comprise a therapeutic polypeptide releasably attached to an exosomal polypeptide. Furthermore, the present invention pertains to manufacturing methods, pharmaceutical compositions, medical uses and applications, and various other embodiments related to the inventive EVs.