Patents by Inventor Joern Kalinowski
Joern Kalinowski has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9719064Abstract: The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type—and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene- and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.Type: GrantFiled: August 1, 2011Date of Patent: August 1, 2017Assignee: BAYER INTELLECTUAL PROPERTY GMBHInventors: Klaus Selber, Bernhard Weingaertner, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
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Publication number: 20170051323Abstract: Surprisingly, it has been found that alkaliphilic bacteria of the genus Corynebacterium are naturally suited to produce L-amino acids.Type: ApplicationFiled: April 16, 2015Publication date: February 23, 2017Applicant: Evonik Degussa GmbHInventors: Ines OCHROMBEL, Brigitte BATHE, Marleen HASSELMEYER, Jörn Kalinowski, Christian Rückert, Marcus Persicke
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Patent number: 9562249Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AICEs from other species.Type: GrantFiled: November 12, 2015Date of Patent: February 7, 2017Assignee: BAYER INTELLECTUAL PROPERTY GMBHInventors: Andreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
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Publication number: 20160130622Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AICEs from other species.Type: ApplicationFiled: November 12, 2015Publication date: May 12, 2016Inventors: Andreas KLEIN, Klaus SELBER, Hermann WEHLMANN, Winfried ROSEN, Alfred PÜHLER, Patrick SCHWIENTEK, Jörn KALINOWSKI, Udo WEHMEIER
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Publication number: 20160053335Abstract: The invention relates to a microorganism that produces and/or secretes an organic chemical compound, wherein the microorganism has an increased expression, compared to the respective starting strain, of a polypeptide LpdA with the activity of a transhydrogenase; and a process for producing an organic chemical compound using the microorganism according to the invention.Type: ApplicationFiled: January 13, 2014Publication date: February 25, 2016Inventors: Frederik Walter, Marcus Persicke, Jörn Kalinowski, Andrea Hüser, Wilfried Claes, Alexander Reth
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Patent number: 9217154Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AiCEs from other species.Type: GrantFiled: December 4, 2012Date of Patent: December 22, 2015Assignee: BAYER INTELLECTUAL PROPERTY GMBHInventors: Andreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
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Publication number: 20140349346Abstract: The present invention is directed to an innate DNA sequence within the complete genome sequence of Actinoplanes sp. SE50/110 which resembles the structure of an actinomycete integrative and conjugative element (AICE). Related AICEs were used for establishing genetic manipulation tools for other bacteria in the past. In this document, we describe the unique features of the specific AICE found in Actinoplanes sp. SE50/110 which are clearly distinct from any other known AICE as a whole, but share minor parts with varying sequence similarity with other characterized AiCEs from other species.Type: ApplicationFiled: December 4, 2012Publication date: November 27, 2014Applicant: BAYER INTELLECTUAL PROPERTY GMBHInventors: Andreas Klein, Klaus Selber, Hermann Wehlmann, Winfried Rosen, Alfred Pühler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
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Publication number: 20130302855Abstract: The present invention describes the DNA-sequence of the wild type genome as well as all genetic modifications which were introduced into the wild type-and further developed strains, based thereon. Thereby the first genotypic characterization of the developed strains, including the latest production strain, has been accomplished, accounting for the major part of the invention. Furthermore, on the basis of the determined DNA-sequences, potential genes were identified and account, combined with their functional annotation, for another part of the invention. In particular, the gene-and DNA-sequences, as well as protein-sequences derived there out, contribute to the invention which were affected by mutagenic modifications throughout the strain development process, potentially contributing to the increased production yield.Type: ApplicationFiled: August 1, 2011Publication date: November 14, 2013Applicant: BAYER INTELLECTUAL PROPERTY GMBHInventors: Klaus Selber, Bernhard Weingaertner, Hermann Wehlmann, Winfried Rosen, Alfred Puhler, Patrick Schwientek, Jörn Kalinowski, Udo Wehmeier
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Patent number: 8334126Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: September 22, 2011Date of Patent: December 18, 2012Assignee: Evonik Degussa GmbHInventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jörn Kalinowski, Alfred Pühler
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Publication number: 20120064582Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: ApplicationFiled: September 22, 2011Publication date: March 15, 2012Inventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jörn Kalinowski, Alfred Pühler
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Patent number: 8048665Abstract: The invention relates to an isolated polynucleotide having a polynucleotide sequence which codes for the alr gene, and a host-vector system having a coryneform host bacterium in which the alr gene is present in attenuated form and a vector which carries at least the alr gene according to SEQ ID No 1, and the use of polynucleotides which comprise the sequences according to the invention as hybridization probes.Type: GrantFiled: November 27, 2002Date of Patent: November 1, 2011Assignee: Evonik Degussa GmbHInventors: Andreas Tauch, Michael Binder, Walter Pfefferle, Georg Thierbach, Jörn Kalinowski, Alfred Pühler
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Patent number: 7759056Abstract: The invention relates to an isolated polynucleotide from coryneform bacteria containing at least one polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide which encodes a polypeptide containing the amino acid sequence according to SEQ ID no. 2, b) polynucleotide which encodes a polypeptide which contains an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID no. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide containing at least 15 successive nucleotides of the polynucleotide sequences of a), b) or c), and to a process for the fermentative production of L-amino acids, in particular L-lysine.Type: GrantFiled: December 29, 2003Date of Patent: July 20, 2010Assignee: Evonik Degussa GmbHInventors: Bettina Möckel, Anke Weissenborn, Walter Pfefferle, Michael Hartmann, Jörn Kalinowski, Alfred Pühler
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Patent number: 7262036Abstract: Disclosed herein is an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO:2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO:2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.Type: GrantFiled: May 22, 2006Date of Patent: August 28, 2007Assignee: Degussa AGInventors: Bettina Hädrich, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
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Patent number: 7132272Abstract: The present invention relates to an isolated polynucleotide coding for the lrp gene and to a process for the fermentative production of L-amino acids by altering the expression of this gene.Type: GrantFiled: April 30, 2002Date of Patent: November 7, 2006Assignee: Degussa AGInventors: Brigitte Bathe, Jörn Kalinowski, Alfred Pühler, Bettina Möckel, Walter Pfefferle
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Patent number: 7118904Abstract: The present invention is directed to nucleotide sequences coding for a bacterial enolase enzyme. These sequences may be used in improved methods for the fermentative preparation of amino acids using coryneform bacteria.Type: GrantFiled: December 8, 2003Date of Patent: October 10, 2006Assignee: Degussa AGInventors: Bettina Möckel, Walter Pfefferle, Thomas Hermann, Alfred Pühler, Jörn Kalinowski, Brigitte Bathe
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Patent number: 7074607Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of a) polynucleotide which is identical to the extent of at least 70% to a polynucleotide which codes for a polypeptide which comprises the amino acid sequence of SEQ ID NO 2, b) polynucleotide which codes for a polypeptide which comprises an amino acid sequence which is identical to the extent of at least 70% to the amino acid sequence of SEQ ID NO 2 c) polynucleotide which is complementary to the polynucleotides of a) and b) and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acid with amplification of the zwa1 gene in the coryneform bacteria employed.Type: GrantFiled: August 14, 2003Date of Patent: July 11, 2006Assignee: Degussa AGInventors: Bettina Möckel, Walter Pfefferle, Achim Marx, Jörn Kalinowski, Brigitte Bathe, Alfred Pühler
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Patent number: 6982162Abstract: The present invention refers to a Corynebacterium glutamicum bacterial strain which new isolated and identified secD and/or secF gene(s) is/are genetically modified in a way that secretion of the bacterial strain is enhanced, the protein- and polynucleotide sequences of these genes, a plasmid containing at least one of these genes and the use of such a bacterial strain for production of a desired substance or in a reporter system.Type: GrantFiled: May 10, 2001Date of Patent: January 3, 2006Assignee: Degussa AGInventors: Stephan Berens, Jörn Kalinowski, Alfred Pühler
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Publication number: 20050266535Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metE gene is present in enhanced form, and the use of the polynucleotide sequences as hybridization probes.Type: ApplicationFiled: June 29, 2005Publication date: December 1, 2005Applicant: DEGUSSA AGInventors: Brigitte Bathe, Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Michael Binder, Dieter Greissinger, Georg Thierbach, Alfred Puehler
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Patent number: 6958228Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.Type: GrantFiled: August 2, 2001Date of Patent: October 25, 2005Assignee: Degussa AGInventors: Brigitte Bathe, Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach
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Publication number: 20050233373Abstract: An isolated polynucleotide comprising a polynucleotide sequence selected from the group consisting of a) polynucleotide which is at least 70% identical to a polynucleotide that codes for a polypeptide which comprises the amino acid sequence of SEQ ID No. 2, b) polynucleotide which codes for a polypeptide that comprises an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID No. 2, c) polynucleotide which is complementary to the polynucleotides of a) or b), and d) polynucleotide comprising at least 15 successive nucleotides of the polynucleotide sequence of a), b) or c), and processes for the fermentative preparation of L-amino acids using coryneform bacteria in which at least the metH gene is present in enhanced form, and use of the polynucleotide sequences as hybridization probes.Type: ApplicationFiled: June 20, 2005Publication date: October 20, 2005Applicant: DEGUSSA AGInventors: Brigitte Bathe, Bettina Moeckel, Walter Pfefferle, Klaus Huthmacher, Christian Rueckert, Joern Kalinowski, Alfred Puehler, Michael Binder, Dieter Greissinger, Georg Thierbach