Abstract: A biologic can be immobilized in a transparent polyurethane-hydrogel composition. Such a polyurethane-hydrogel composition can be prepared from forming a polyurethane-hydrogel mixture and immobilizing a biologic in the mixture. The mixture can be formed by admixing a prepolymer and a water-soluble crosslinker in aqueous solvent and in the substantial absence of organic solvent. A suitable prepolymer generally includes at least one water-soluble polyol and at least one isocyanate and can be added to the mixture in an amount of no greater than about 5 weight percent based on total weight of all components. A suitable crosslinker generally has a crosslinker functionality of at least 2. A biologic includes cells, peptides, nucleics, peptide nucleic acids, saccharides, lipopolysaccharides, glycolipids, and combinations of these. A polyurethane-hydrogel composition having an immobilized biologic can be particularly useful for biomedical applications.