Patents by Inventor Johan A. Van den Berg

Johan A. Van den Berg has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • DNA constructs containing a kluyveromyces .alpha. factor leader sequence for directing secretion of heterologous polypeptides
    Patent number: 5217891
    Abstract: DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces .alpha.-factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis .alpha.-factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.
    Type: Grant
    Filed: April 9, 1990
    Date of Patent: June 8, 1993
    Assignees: Chiron Corporation, Gist-Brocades
    Inventors: Anthony J. Brake, Johan A. Van Den Berg
  • DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
    Patent number: 5010182
    Abstract: DNA constructs that are useful for providing secretory expression of heterologous polypeptides in yeast comprising a DNA sequence that includes a Kluyveromyces .alpha.-factor leader sequence linked to the heterologous polypeptide by a yeast processing signal. Constructs employing the K. lactis .alpha.-factor leader and processing signal sequences, with and without spacer, linked to prochymosin are exemplified.
    Type: Grant
    Filed: July 28, 1987
    Date of Patent: April 23, 1991
    Assignees: Chiron Corporation, Gist-Brocades NV
    Inventors: Anthony J. Brake, Johan A. Van Den Berg
  • Kluyveromyces as a host strain
    Patent number: 4943529
    Abstract: Kluyveromyces hosts and DNA expression cassettes for use in Kluyveromyces are provided for transcription of endogenous and/or exogenous DNA, and production of peptides, for enhancing production of an endogenous product, or producing an exogenous product. The Kluyveromyces hosts find particular use for secretion of a desired peptide product, where signal sequences may be native to the peptide or provided from endogenous or exogenous signal sequences, including synthetic sequences, functional in Kluyveromyces. A transformation procedure is provided for efficiently transforming Kluyveromyces.
    Type: Grant
    Filed: July 28, 1987
    Date of Patent: July 24, 1990
    Assignee: Gist-brocades NV
    Inventors: Johan A. Van den Berg, Albert J. J. Van Ooyen, Krijn Rietveld