Patents by Inventor Johan Desmet
Johan Desmet has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10087249Abstract: The invention provides single-chain Alphabody library comprising at least 100 different-sequence single-chain Alphabody polypeptides, wherein said Alphabody polypeptides differ from each other in at least one of a defined set of 5 to 20 variegated amino acid residue positions, and wherein at least 70% but not all of said variegated amino acid residue positions are located either in the loop, helix surface or linker region of the Alphabody. The invention further provides methods for use of the Alphabody libraries and Alphabodies obtainable by the methods of the invention.Type: GrantFiled: March 11, 2016Date of Patent: October 2, 2018Assignee: COMPLIX SAInventors: Johan Desmet, Ignace Lasters, Maria Henderikx, Anita Wehnert
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Patent number: 10023647Abstract: Provided herein are polypeptides that are capable of crossing the cellular membrane and entering into the intracellular environment, which polypeptides are suitable for use in prophylactic, therapeutic or diagnostic applications as well as in screening and detection. Nucleic acids encoding such polypeptides; methods for preparing such polypeptides, host cells expressing or capable of expressing such polypeptides, compositions, and in particular pharmaceutical compositions, that comprise such polypeptides, in particular for prophylactic, therapeutic or diagnostic purposes are also provided.Type: GrantFiled: October 22, 2013Date of Patent: July 17, 2018Assignee: COMPLIX NVInventors: Stefan Loverix, Philippe Alard, Sabrina Deroo, Klaartje Somers, Ignace Lasters, Johan Desmet
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Patent number: 9701717Abstract: The application provides Alphabodies that specifically bind to IL-23, as well as to polypeptides that comprise or essentially consist of such Alphabodies. Also provided herein are nucleic acids encoding such Alphabodies; to methods for preparing such Alphabodies and polypeptides; and in particular to pharmaceutical compositions, that comprise such Alphabodies, polypeptides, nucleic acids and/or host cells.Type: GrantFiled: October 22, 2013Date of Patent: July 11, 2017Assignee: COMPLIX NVInventors: Johan Desmet, Maria Paulina Henderikx, Klaartje Somers, Stefan Loverix, Mark Vaeck, Ignace Lasters
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Publication number: 20160326242Abstract: The invention provides single-chain Alphabody library comprising at least 100 different-sequence single-chain Alphabody polypeptides, wherein said Alphabody polypeptides differ from each other in at least one of a defined set of 5 to 20 variegated amino acid residue positions, and wherein at least 70% but not all of said variegated amino acid residue positions are located either in the loop, helix surface or linker region of the Alphabody. The invention further provides methods for use of the Alphabody libraries and Alphabodies obtainable by the methods of the invention.Type: ApplicationFiled: March 11, 2016Publication date: November 10, 2016Inventors: Johan Desmet, Ignace Lasters, Maria Henderikx, Anita Wehnert
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Patent number: 9315805Abstract: The invention provides single-chain Alphabody library comprising at least 100 different-sequence single-chain Alphabody polypeptides, wherein said Alphabody polypeptides differ from each other in at least one of a defined set of 5 to 20 variegated amino acid residue positions, and wherein at least 70% but not all of said variegated amino acid residue positions are located either in the loop, helix surface or linker region of the Alphabody. The invention further provides methods for use of the Alphabody libraries and Alphabodies obtainable by the methods of the invention.Type: GrantFiled: January 6, 2011Date of Patent: April 19, 2016Assignee: Complix SAInventors: Johan Desmet, Ignace Lasters, Maria Henderikx, Anita Wehnert
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Publication number: 20160039867Abstract: The present invention relates to single-chain proteins of the formula HRS1-L1-HRS2-L2-HRS3, wherein HRS1, HRS2 and HRS3 are heptad repeat sequences and L1 and L2 are structurally flexible linker sequences, and wherein HRS1, HRS2 and HRS3 form a thermodynamically stable triple-stranded, antiparallel, alpha-helical coiled coil structure in aqueous solution. The invention also relates to amino acid sequence variants, conditions and methods to obtain such proteins and variants, and usages thereof, especially their usage as scaffolds and as therapeutic products.Type: ApplicationFiled: August 21, 2015Publication date: February 11, 2016Applicant: Complix NVInventors: Johan Desmet, Ignace Joseph Isabella Lasters, Stefan Loverix
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Patent number: 9217011Abstract: The present invention is related to a non-natural, thermodynamically stable, proteinaceous scaffold consisting of three non-covalently associated peptides, wherein each peptide sequence comprises less than fifty amino acid residues and wherein at least 50% of the said residues are substitutable amino acids into at least ten different amino acid residue types. The present invention is further related to a non-natural, triple-stranded, parallel alpha-helical coiled coil scaffold wherein each of the three constituting peptide sequences comprise between 2 and 7 consecutive heptad repeats of the formula cxxcxxx (SEQ ID NO: 10), wherein at least 70% of the core c-residues are isoleucines, wherein all non-core x-residues are alanines, and wherein the constituting peptide sequences remain associated under physical conditions that are significantly different from physiological conditions.Type: GrantFiled: September 8, 2008Date of Patent: December 22, 2015Assignee: Complix NVInventors: Johan Desmet, Ignace Lasters
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Publication number: 20150266925Abstract: The application provides Alphabodies that specifically bind to IL-23, as well as to polypeptides that comprise or essentially consist of such Alphabodies. Also provided herein are nucleic acids encoding such Alphabodies; to methods for preparing such Alphabodies and polypeptides; and in particular to pharmaceutical compositions, that comprise such Alphabodies, polypeptides, nucleicacids and/or host cells.Type: ApplicationFiled: October 22, 2013Publication date: September 24, 2015Applicant: COMPLIX NVInventors: Johan Desmet, Maria Paulina Henderikx, Klaartje Somers, Stefan Loverix, Mark Vaeck, Ignace Lasters
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Publication number: 20150266968Abstract: Provided herein are polypeptides that are capable of crossing the cellular membrane and entering into the intracellular environment, which polypeptides are suitable for use in prophylactic, therapeutic or diagnostic applications as well as in screening and detection. Nucleic acids encoding such polypeptides; methods for preparing such polypeptides, host cells expressing or capable of expressing such polypeptides, compositions, and in particular pharmaceutical compositions, that comprise such polypeptides, in particular for prophylactic, therapeutic or diagnostic purposes are also provided.Type: ApplicationFiled: October 22, 2013Publication date: September 24, 2015Inventors: Stefan Loverix, Philippe Alard, Sabrina Deroo, Klaartje Somers, Ignace Lasters, Johan Desmet
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Publication number: 20140363434Abstract: The application provides polypeptides comprising or essentially consisting of at least one Alphabody, wherein said Alphabody is capable of internalization into a cell and specifically binds to an intracellular target molecule. The application further provides nucleic acids encoding such polypeptides; methods for preparing such polypeptides; host cells expressing or capable of expressing such polypeptides; compositions, and in particular to pharmaceutical compositions, that comprise such polypeptides, nucleic acids and/or host cells; and uses of such polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes.Type: ApplicationFiled: January 4, 2013Publication date: December 11, 2014Applicant: COMPLIX NVInventors: Ignace Lasters, Mark Vaeck, Johan Desmet, Jürgen Debaveye, Sabrina Deroo, Stefan Loverix
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Publication number: 20140294828Abstract: The invention provides methods for the production of single-chain Alphabody polypeptides having detectable binding affinity for, or detectable in vitro activity on, a viral protein of interest, which comprising the step of producing a single-chain Alphabody library comprising at least 100 different-sequence single-chain Alphabody polypeptides, wherein said Alphabody polypeptides differ from each other in at least one of a defined set of 5 to 20 variegated amino acid residue positions, and wherein said variegated amino acid residue positions are located at specific positions in one or more of the alpha-helices of the Alphabody or the linker fragment connecting two consecutive alpha-helices of the Alphabody polypeptides. The invention further provides Alphabodies obtainable by the methods of the invention and uses thereof.Type: ApplicationFiled: January 6, 2011Publication date: October 2, 2014Applicant: COMPLIX SAInventors: Johan Desmet, Ignace Lasters, Geert Meersseman, Sabrina Deroo
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Publication number: 20140066601Abstract: The invention provides single-chain Alphabody library comprising at least 100 different-sequence single-chain Alphabody polypeptides, wherein said Alphabody polypeptides differ from each other in at least one of a defined set of 5 to 20 variegated amino acid residue positions, and wherein at least 70% but not all of said variegated amino acid residue positions are located either in the loop, helix surface or linker region of the Alphabody. The invention further provides methods for use of the Alphabody libraries and Alphabodies obtainable by the methods of the invention.Type: ApplicationFiled: January 6, 2011Publication date: March 6, 2014Inventors: Johan Desmet, Ignace Lasters, Maria Henderikx, Anita Wehnert
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Publication number: 20140057830Abstract: Alphabodies that specifically bind to cytokines or growth factor and/or their receptors, as well as polypeptides that comprise or essentially consist of such Alphabodies. Further nucleic acids encoding such Alphabodies; methods for preparing such Alphabodies and polypeptides; host cells expressing or capable of expressing such Alphabodies and polypeptides; compositions, and in particular pharmaceutical compositions, that comprise such Alphabodies, polypeptides, nucleic acids and/or host cells; and uses of such Alphabodies or polypeptides, nucleic acids, host cells and/or compositions, in particular for prophylactic, therapeutic or diagnostic purposes.Type: ApplicationFiled: January 6, 2012Publication date: February 27, 2014Applicant: COMPLIX SAInventors: Ignace Lasters, Johan Desmet, Maria Henderikx, Anita Wehnert, Geert Meersseman
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Patent number: 8571806Abstract: The present invention provides methods for generating information related to the molecular structure of a biomolecule.Type: GrantFiled: December 2, 2010Date of Patent: October 29, 2013Assignee: Lonza Biologies PLCInventors: Johan Desmet, Ignace Lasters, Dominique Vlieghe, Carlo Boutton, Philippe Stas, Jan Spriet
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ALPHABODIES SPECIFICALLY BINDING TO CLASS-I VIRAL FUSION PROTEINS AND METHODS FOR PRODUCING THE SAME
Publication number: 20130261049Abstract: Single-chain Alphabodies that comprise an alpha-helical binding region which mediates binding to a first fusion-driving region of a class-1 viral fusion protein and which structurally mimics a second fusion-driving region of said class-1 viral fusion protein, wherein said first and second fusion-driving regions of said class-1 viral fusion protein are regions which interact to drive the fusion between a virus displaying said class-1 viral fusion protein and a target cell.Type: ApplicationFiled: December 2, 2011Publication date: October 3, 2013Applicant: COMPLIX SAInventors: Johan Desmet, Ignace Lasters, Geert Meersseman, Sabrina Deroo -
Patent number: 8538706Abstract: The present invention is related to a quantitative structure-based affinity scoring method for peptide/protein complexes. More specifically, the present invention comprises a method that operates on the basis of a highly specific force field function (e.g. CHARMM) that is applied to all-atom structural representations of peptide/receptor complexes. Peptide side-chain contributions to total affinity are scored after detailed rotameric sampling followed by controlled energy refinement. The method of the invention further comprises a de novo approach to estimate dehydration energies from the simulation of individual amino acids in a solvent box filled with explicit water molecules and applying the same force field function as used to evaluate peptide/receptor complex interactions.Type: GrantFiled: October 11, 2011Date of Patent: September 17, 2013Assignee: Lonza Biologics PLCInventors: Johan Desmet, Geert Meersseman, Nathalie Boutonnet, Jurgen Pletinckx, Krista De Clercq, Ignace Lasters
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Publication number: 20130211808Abstract: The present invention provides methods and apparatus for analyzing a protein structure.Type: ApplicationFiled: May 15, 2012Publication date: August 15, 2013Applicant: Lonza Biologics plcInventors: Johan DESMET, Ignace LASTERS, Dominique VLIEGHE, Carlo BOUTTON, Philippe STAS
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Publication number: 20130013279Abstract: The present invention provides methods and apparatus for analyzing a protein structure.Type: ApplicationFiled: May 15, 2012Publication date: January 10, 2013Applicant: Lonza Biologics plcInventors: Johan Desmet, Ignace Lasters, Dominique Vlieghe, Carlo Boutton, Philippe Stas
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Publication number: 20120278054Abstract: The present invention is related to a quantitative structure-based affinity scoring method for peptide/protein complexes. More specifically, the present invention comprises a method that operates on the basis of a highly specific force field function (e.g. CHARMM) that is applied to all-atom structural representations of peptide/receptor complexes. Peptide side-chain contributions to total affinity are scored after detailed rotameric sampling followed by controlled energy refinement. The method of the invention further comprises a de novo approach to estimate dehydration energies from the simulation of individual amino acids in a solvent box filled with explicit water molecules and applying the same force field function as used to evaluate peptide/receptor complex interactions.Type: ApplicationFiled: October 11, 2011Publication date: November 1, 2012Applicant: Lonza Biologics plcInventors: Johan Desmet, Geert Meersseman, Nathalie Boutonnet, Jurgen Pletinckx, Krista De Clercq, Ignace Lasters
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Publication number: 20120202247Abstract: The present invention relates to a method for structure-based prediction of properties of peptides and peptide analogs in complex with major histocompatibility (MHC) class I and class II molecules. The said properties mainly relate to the three-dimensional structure of an MHC/peptide complex and the binding affinity of a peptide for an MHC receptor. The invention further relates to a computer program and a device therefor. The invention further relates to data produced by a method of the invention. The invention further relates to peptides and peptide analogs predicted to bind to target-MHC molecules. The present invention thus relates to the field of immunology, with possible applications in manufacture of vaccinates, de-immunization of proteins, and manufacture of therapeutic agents, especially immuno-therapeutic agents.Type: ApplicationFiled: March 27, 2012Publication date: August 9, 2012Applicant: Algonomics N.V.Inventors: Ignace Lasters, Johan Desmet