Patents by Inventor Johan Jochem Bruggeman

Johan Jochem Bruggeman has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 7951551
    Abstract: The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
    Type: Grant
    Filed: April 8, 2008
    Date of Patent: May 31, 2011
    Assignee: PerkinElmer Life and Analytical Sciences B.V.
    Inventors: Harry van Lune, Johan Jochem Bruggeman
  • Publication number: 20090053744
    Abstract: The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
    Type: Application
    Filed: April 8, 2008
    Publication date: February 26, 2009
    Applicant: PerkinElmer Life and Analytical Sciences B.V.
    Inventors: Harry van Lune, Johan Jochem Bruggeman
  • Publication number: 20090047693
    Abstract: The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
    Type: Application
    Filed: September 12, 2008
    Publication date: February 19, 2009
    Applicant: PerkinElmer Life and Analytical Sciences B.V.
    Inventors: Harry van Lune, Johan Jochem Bruggeman
  • Patent number: 7425422
    Abstract: The invention relates to methods and kits for detecting enzyme activity using bioluminescence. In particular, it relates to a novel assay system with increased light yield for a sensitive and convenient detection of luciferase activity, such as luciferase reporter enzyme activity. Provided is a method of detecting luciferase activity in a sample, comprising incubating the sample in the presence of luciferin and ATP to allow the generation of a light signal, wherein said light signal is enhanced by performing the incubation in a reaction mixture comprising phosphate and ammonium ions, and measuring the light signal. The invention also relates to kits for use in such method.
    Type: Grant
    Filed: May 12, 2006
    Date of Patent: September 16, 2008
    Assignee: PerkinElmer Life and Analytical Sciences B.V.
    Inventors: Harry van Lune, Johan Jochem Bruggeman