Patents by Inventor Johan Pihl

Johan Pihl has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 8232074
    Abstract: The present invention relates to methods of measuring electrical properties of a cell using electrode devices comprising tapered nanotips having submicrometer dimensions (“nanoelectrodes”) for insertion into a cell. The devices are used to measure electrical properties of the cell and, optionally, may be used to electroporate, the cell or subcellular structures within the cell. The invention also provides arrays of electrode devices having nanotips for simultaneously or sequentially measuring the electrical properties of cells (e.g., such as surface immobilized cells). The electrodes can be used to measure properties of ion channels and in HTS assays to identify drugs which affect the properties of ion channels. The invention additionally provides microfluidic systems adapted for use with the electrode devices having nanotips. In combination with the electrodes, the microfluidic systems provide cell-based biosensors for monitoring cellular responses to conditions, such as exposure to candidate drugs.
    Type: Grant
    Filed: October 15, 2003
    Date of Patent: July 31, 2012
    Assignee: Cellectricon AB
    Inventors: Kent Jardemark, Aldo Jesorka, Mattias Karlsson, Jessica Olofsson, Owe Orwar, Johan Pihl, Eskil Sahlin
  • Publication number: 20120040370
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors.
    Type: Application
    Filed: October 25, 2006
    Publication date: February 16, 2012
    Applicant: CELLECTRICON AB
    Inventors: Owe Orwar, Daniel Chiu, Mattias Karlsson, Johan Pihl, Jon Sinclair, Frederik Pettersson
  • Patent number: 8057754
    Abstract: Apparatuses and methods of aligning at least one tip of a tip manifold with a plurality of wells of a multiwell plate. The tip manifold includes a plate, at least one tip depending from the plate, a first tip alignment pin depending from the plate, and a second tip alignment pin depending from the plate. The second tip alignment pin opposes the first tip alignment pin. The multiwell plate includes a body defining a plurality of non-porous wells for holding biological material, a first alignment hole, and a second alignment hole. The second alignment hole opposes the first alignment hole.
    Type: Grant
    Filed: March 10, 2009
    Date of Patent: November 15, 2011
    Assignee: Cellectricon AB
    Inventors: Johan Pihl, Mattias Karlsson
  • Publication number: 20110183407
    Abstract: Apparatuses and methods of aligning at least one tip of a tip manifold with a plurality of wells of a multiwell plate. The tip manifold includes a plate, at least one tip depending from the plate, a first tip alignment pin depending from the plate, and a second tip alignment pin depending from the plate. The second tip alignment pin opposes the first tip alignment pin. The multiwell plate includes a body defining a plurality of non-porous wells for holding biological material, a first alignment hole, and a second alignment hole. The second alignment hole opposes the first alignment hole.
    Type: Application
    Filed: March 10, 2009
    Publication date: July 28, 2011
    Applicant: CELLECTRICON AB
    Inventors: Johan Pihl, Mattias Karlsson
  • Publication number: 20110091864
    Abstract: Disclosed herein is a device comprising at least one supporting solid surface comprising at least one membraneophilic region; a covering layer that is at least partially immobilized to the membraneophilic region, said covering layer consisting of (i) a surfactant membrane, (ii) a lipid mimicking polymer, (iii) a surfactant or emulsion system or (iv) a liquid crystal; and a substance included in or bound to, connected to or associated with the covering layer. Also disclosed are methods wherein the device is used and use of the device.
    Type: Application
    Filed: December 23, 2005
    Publication date: April 21, 2011
    Inventors: Anders Karlsson, Roger Karlsson, Johan Pihl, Mattias Karlsson, Owe Orwar, Tatiana Lobovkina, Max Davidson, Aldo Jesorka
  • Patent number: 7905348
    Abstract: The present invention relates to a card assembly comprising a cover and a data storage means. The cover comprises a back sheet, a front sheet and an intermediate sheet, which are integral and connected by one folding line area respectively. The intermediate sheet has a through hole adapted to the data storage means and the data storage means is placed in the through hole. The back sheet, the front sheet and the intermediate sheet are folded together along the folding line areas and permanently attached together, wherein the data storage means is enclosed in a pocket. The back sheet has a tear portion, which is operable to open the pocket and expose a part of the data storage means for enabling removal of the data storage means from the pocket and reinsertion thereof into the pocket. The present invention also relates to a blank for a cover for such a card assembly and to method for manufacturing such a card assembly.
    Type: Grant
    Filed: December 17, 2004
    Date of Patent: March 15, 2011
    Assignee: Tracks Trade & Publishing AB
    Inventors: Peter Gustafsson, Johan Pihl
  • Publication number: 20100143944
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.
    Type: Application
    Filed: February 25, 2009
    Publication date: June 10, 2010
    Applicant: Cellectricon AB
    Inventors: Owe Orwar, Daniel Chiu, Johan Pihl, Jon Sinclair, Jessica Olofsson, Mattias Karlsson, Kent Jardemark
  • Publication number: 20090286297
    Abstract: Apparatuses and methods of aligning at least one tip of a tip manifold with a plurality of wells of a multiwell plate. The tip manifold includes a plate, at least one tip depending from the plate, a first tip alignment pin depending from the plate, and a second tip alignment pin depending from the plate. The second tip alignment pin opposes the first tip alignment pin. The multiwell plate includes a body defining a plurality of non-porous wells for holding biological material, a first alignment hole, and a second alignment hole. The second alignment hole opposes the first alignment hole.
    Type: Application
    Filed: March 10, 2009
    Publication date: November 19, 2009
    Applicant: CELLECTRICON AB
    Inventors: Johan Pihl, Mattias Karlsson
  • Patent number: 7563614
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.
    Type: Grant
    Filed: January 15, 2003
    Date of Patent: July 21, 2009
    Assignee: Cellectricon AB
    Inventors: Owe Orwar, Daniel Chiu, Johan Pihl, Jon Sinclair, Jessica Olofsson, Mattias Karlsson, Kent Jardemark
  • Publication number: 20090047676
    Abstract: The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording.
    Type: Application
    Filed: June 6, 2008
    Publication date: February 19, 2009
    Applicant: Cellectricon AB
    Inventors: Mattias Karlsson, Owe Orwar, Daniel T. Chiu, Jon Sinclair, Kent Jardemark, Jessica Olofsson, Johan Pihl, Cecilia Farre
  • Patent number: 7470518
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors.
    Type: Grant
    Filed: January 6, 2005
    Date of Patent: December 30, 2008
    Assignee: Cellectricon AB
    Inventors: Daniel Chiu, Owe Orwar, Kent Jardemark, Mattias Karlsson, Jessica Olofsson, Johan Pihl, Jon Sinclair
  • Patent number: 7390650
    Abstract: The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording.
    Type: Grant
    Filed: August 20, 2003
    Date of Patent: June 24, 2008
    Assignee: Cellectricon AB
    Inventors: Mattias Karlsson, Owe Orwar, Daniel T. Chiu, Jon Sinclair, Kent Jardemark, Jessica Olofsson, Johan Pihl, Cecilia Farre
  • Publication number: 20060234298
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors.
    Type: Application
    Filed: January 6, 2005
    Publication date: October 19, 2006
    Inventors: Daniel Chiu, Owe Orwar, Kent Jardemark, Mattias Karlsson, Jessica Olofsson, Johan Pihl, Jon Sinclair
  • Publication number: 20060223164
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.
    Type: Application
    Filed: January 15, 2003
    Publication date: October 5, 2006
    Inventors: Owe Orwar, Daniel Chiu, Johan Pihl, Jon Sinclair, Jessica Olofsson, Mattias Karlsson, Kent Jardemark
  • Publication number: 20060078961
    Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.
    Type: Application
    Filed: January 14, 2003
    Publication date: April 13, 2006
    Inventors: Daniel Chiu, Owe Orwar, Kent Jardemark, Mattias Karlsson, Jessica Olofsson, Johan Pihl, Jon Sinclair
  • Publication number: 20060006080
    Abstract: The present invention relates to a card assembly comprising a cover and a data storage means. The cover comprises a back sheet, a front sheet and an intermediate sheet, which are integral and connected by one folding line area respectively. The intermediate sheet has a through hole adapted to the data storage means and the data storage means is placed in the through hole. The back sheet, the front sheet and the intermediate sheet are folded together along the folding line areas and permanently attached together, wherein the data storage means is enclosed in a pocket. The back sheet has a tear portion, which is operable to open the pocket and expose a part of the data storage means for enabling removal of the data storage means from the pocket and reinsertion thereof into the pocket. The present invention also relates to a blank for a cover for such a card assembly and to method for manufacturing such a card assembly.
    Type: Application
    Filed: December 17, 2004
    Publication date: January 12, 2006
    Applicant: Tracks Trade and Publishing AB
    Inventors: Peter Gustafsson, Johan Pihl
  • Publication number: 20040182707
    Abstract: The present invention relates to methods of measuring electrical properties of a cell using electrode devices comprising tapered nanotips having submicrometer dimensions (“nanoelectrodes”) for insertion into a cell. The devices are used to measure electrical properties of the cell and, optionally, may be used to electroporate, the cell or subcellular structures within the cell. The invention also provides arrays of electrode devices having nanotips for simultaneously or sequentially measuring the electrical properties of cells (e.g., such as surface immobilized cells). The electrodes can be used to measure properties of ion channels and in HTS assays to identify drugs which affect the properties of ion channels. The invention additionally provides microfluidic systems adapted for use with the electrode devices having nanotips.
    Type: Application
    Filed: October 15, 2003
    Publication date: September 23, 2004
    Applicant: Cellectricon AB
    Inventors: Kent Jardemark, Aldo Jesorka, Mattias Karlsson, Jessica Olofsson, Owe Orwar, Johan Pihl, Eskil Sahlin
  • Publication number: 20040110307
    Abstract: The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording.
    Type: Application
    Filed: August 20, 2003
    Publication date: June 10, 2004
    Inventors: Mattias Karlsson, Owe Orwar, Daniel T. Chiu, Jon Sinclair, Kent Jardemark, Jessica Olofsson, Johan Pihl, Cecilia Farre
  • Patent number: D598128
    Type: Grant
    Filed: March 12, 2008
    Date of Patent: August 11, 2009
    Assignee: Cellectricon AB
    Inventors: Johan Pihl, Mattias Karlsson