Abstract: A method and apparatus are provided, in which an observation volume is defined by a volume where light from illumination means and a field of view of detection means overlap. The central axes of said light from the illumination means and said field of view of the detection means are non-parallel, and the sample is transported through the observation volume during imaging, preferably by rotation of a sample container holding the sample.

Abstract: An apparatus for imaging a includes an light source for illuminating the sample simultaneously in a line focus or an array of foci; and a sensor for detecting photons emitted or scattered from the sample simultaneously in an array of fields of view. An array of sub-observation volumes in the sample, from which photons are emitted or scattered during imaging, is defined by the volumes in space where the line focus or array of foci from the light source overlap with the corresponding array of field of views of the sensor. A cylindrical sample holder holds the sample at a surface and is rotatably arranged such that at least a portion of the sample can be transported through at least one of the sub-observation volumes by rotating the sample holder. The apparatus can be used in a high-throughput method of imaging.

Abstract: An apparatus for imaging a includes an light source for illuminating the sample simultaneously in a line focus or an array of foci; and a sensor for detecting photons emitted or scattered from the sample simultaneously in an array of fields of view. An array of sub-observation volumes in the sample, from which photons are emitted or scattered during imaging, is defined by the volumes in space where the line focus or array of foci from the light source overlap with the corresponding array of field of views of the sensor. A cylindrical sample holder holds the sample at a surface and is rotatably arranged such that at least a portion of the sample can be transported through at least one of the sub-observation volumes by rotating the sample holder. The apparatus can be used in a high-throughput method of imaging.

Abstract: A method and apparatus are provided, in which an observation volume is defined by a volume where light from illumination means and a field of view of detection means overlap. The central axes of said light from the illumination means and said field of view of the detection means are non-parallel, and the sample is transported through the observation volume during imaging, preferably by rotation of a sample container holding the sample.

Abstract: The present invention provides a FCCS method for determining the concentration and/or the diffusion coefficient of at least a first labeled species, a second labeled species and/or a complex between said first and second labeled species, in a system, wherein the method comprises the steps of determining a cross-talk parameter K, wherein K is the ratio between the brightness of the first labeled species and the second labeled species at the centre of each focus, as detected for both species in the channel for detecting the second labeled species; using the cross talk parameter K for determining a displacement parameter ro and using K, ro, or both K and ro for determining the concentration and/or the diffusion coefficient of said first and/or a second labeled species and/or a complex between said first and second labeled species.