Patents by Inventor Johann-Peter Thalhofer
Johann-Peter Thalhofer has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Publication number: 20230052545Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: ApplicationFiled: August 26, 2022Publication date: February 16, 2023Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Patent number: 11459556Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: GrantFiled: May 4, 2020Date of Patent: October 4, 2022Assignee: Roche Diagnostics Operations, Inc.Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Publication number: 20200263160Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: ApplicationFiled: May 4, 2020Publication date: August 20, 2020Applicant: Roche Diagnostics Operations, Inc.Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Patent number: 10227642Abstract: The present invention relates to a formulation of a thermostable DNA polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (PCR). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step.Type: GrantFiled: January 21, 2014Date of Patent: March 12, 2019Assignee: Roche Diagnostics Operations, Inc.Inventors: Ulrike Fischer, Michael Greif, Harald Sobek, Johann-Peter Thalhofer
-
Publication number: 20170247679Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: ApplicationFiled: May 15, 2017Publication date: August 31, 2017Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Patent number: 9663769Abstract: The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference.Type: GrantFiled: October 1, 2015Date of Patent: May 30, 2017Assignee: Roche Diagnostics Operations, Inc.Inventors: Michael Greif, Christian Rudolph, Manfred Schmidt, Harald Sobek, Johann-Peter Thalhofer
-
Patent number: 9574186Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: GrantFiled: April 28, 2015Date of Patent: February 21, 2017Assignee: Roche Diagnostics Operations, Inc.Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Publication number: 20160319268Abstract: Described are compositions, in particular lyophilizates, containing proteolytic enzymes, and methods for producing the compositions. Typically these compositions contain one or more proteases with collagenase activity and a neutral protease, for example, thermolysin. The compositions are free of acetate salts. Surprisingly, such compositions can be dissolved in water more rapidly than lyophilized protease mixtures of the state of the art.Type: ApplicationFiled: April 29, 2015Publication date: November 3, 2016Inventors: Werner Hoelke, Michaela Fischer, Johann-Peter Thalhofer, Markus Weber
-
Publication number: 20160032260Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.Type: ApplicationFiled: October 16, 2015Publication date: February 4, 2016Inventors: Harald Sobek, Johann-Peter Thalhofer, Rainer Mueller, Manfred Schmidt, Michael Greif, Armin Ruf, Christian Rudolph
-
Publication number: 20160032261Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.Type: ApplicationFiled: October 16, 2015Publication date: February 4, 2016Inventors: Harald Sobek, Johann-Peter Thalhofer, Rainer Mueller, Manfred Schmidt, Michael Greif, Armin Ruf, Christian Rudolph
-
Publication number: 20160010069Abstract: The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference.Type: ApplicationFiled: October 1, 2015Publication date: January 14, 2016Inventors: Michael Greif, Christian Rudolph, Manfred Schmidt, Harald Sobek, Johann-Peter Thalhofer
-
Publication number: 20150337282Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: ApplicationFiled: April 28, 2015Publication date: November 26, 2015Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Patent number: 9193959Abstract: The present invention provides improved variants of T7 RNA polymerase by introducing novel mutations which lead to improved thermostability of the enzyme. According to the invention, amino acid substitutions at the positions Val426, Ser633, Val650, Thr654, Ala702, Val795, and combinations thereof are advantageous.Type: GrantFiled: March 23, 2011Date of Patent: November 24, 2015Assignee: Roche Diagnostics Operations, Inc.Inventors: Harald Sobek, Johann-Peter Thalhofer, Rainer Mueller, Manfred Schmidt, Michael Greif, Armin Ruf, Christian Rudolph
-
Patent number: 9045743Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilizate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: GrantFiled: June 5, 2012Date of Patent: June 2, 2015Assignee: Roche Diagnostic Operations, Inc.Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Publication number: 20140134633Abstract: The present invention relates to a formulation of a thermostable DNA polymerase which is completely free of detergents and its particular use in real time polymerase chain reaction (PCR). Such a formulation may be obtained if the selected purification method does not require the addition of a detergent at any purification step.Type: ApplicationFiled: January 21, 2014Publication date: May 15, 2014Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.Inventors: Ulrike Fischer, Michael Greif, Harald Sobek, Johann-Peter Thalhofer
-
Patent number: 8476053Abstract: The present invention provides an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase. The present invention further provides a method of stabilizing an aqueous composition comprising a protein with enzymatic activity of alpha-galactosidase, and a method of preparing a purified aqueous composition comprising the protein with enzymatic activity of alpha-galactosidase.Type: GrantFiled: April 19, 2010Date of Patent: July 2, 2013Assignee: Roche Diagnostics Operations, Inc.Inventors: Harald Sobek, Manfred Schmidt, Rainer Mueller, Werner Hoelke, Johann-Peter Thalhofer
-
Publication number: 20120252071Abstract: The present disclosure provide novel variants of T7 RNA polymerase. Embodiments of T7 variants, according to the instant invention, include a Cysteine-Serine substitution on position 723 of the amino acid sequence of the T7 polypeptide. Embodiments of T7 variants according to the instant invention have a DNA-dependent RNA polymerase enzymatic activity and a reduced tendency to form intramolecular homodimers by way of oxidizing thiol groups. The amino acid substitutions within the T7 variants disclosed herein impact minimally, if at all, the RNA polymerase activity of the T7 polypeptide. Further, the mutations of the disclosed embodiments may optionally be combined with mutations which provide enhanced thermostability compared to the wild-type reference.Type: ApplicationFiled: March 30, 2012Publication date: October 4, 2012Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.Inventors: Michael Greif, Christian Rudolph, Manfred Schmidt, Harald Sobek, Johann-Peter Thalhofer
-
Publication number: 20120244598Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: ApplicationFiled: June 5, 2012Publication date: September 27, 2012Applicant: ROCHE DIAGNOSTICS OPERATIONS, INC.Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber
-
Patent number: 8236356Abstract: The present invention provides improved media for the cultivation of Clostridium histolyticum and culture supernatants for the biotechnological production of collagenase enzymes. The nutrient media according to the invention comprise one or more peptones from a non-mammalian source, preferably plant-derived peptones. The media can additionally comprise fish gelatin. The invention provides media, culture supernatants comprising Clostridium histolyticum collagenase, and methods to produce said collagenase.Type: GrantFiled: June 4, 2009Date of Patent: August 7, 2012Assignee: Roche Diagnostics Operations, Inc.Inventors: Bernhard Suppmann, Werner Hoelke, Artur Hoffmann, Thomas Marx, Kirsten Sonn, Johann-Peter Thalhofer
-
Patent number: 8211684Abstract: The present invention deals with the proteolytic enzyme thermolysin which tends to be unstable in aqueous solution. The invention provides methods and compositions to enhance the stability of dissolved thermolysin in aqueous solution. Thermolysin, crude thermolysin or a lyophilisate containing thermolysin and one or more salts, is contacted with an aqueous buffer with a low salt concentration and a first solution is formed. Subsequently, a further salt in solid form is added and dissociated, thereby forming a second solution comprising thermolysin in a stabilized form.Type: GrantFiled: August 17, 2009Date of Patent: July 3, 2012Assignee: Roche Diagnostics Operations, Inc.Inventors: Werner Hoelke, Johann-Peter Thalhofer, Antje Liehre, Markus Weber