Patents by Inventor Johanna Gramsall

Johanna Gramsall has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Cholesteroloxidase and method for its production from microorganisms
    Patent number: 4144129
    Abstract: A new enzyme, cholesteroloxidase, is prepared by recovering same from microorganisms, preferably by washing a cholesterol-converting microorganism with butanol/water, disintegrating the microorganism culture and extracting same with a buffer solution containing a non-ionic surface-active agent, and isolating cholesterol oxidase from the extract.
    Type: Grant
    Filed: May 15, 1973
    Date of Patent: March 13, 1979
    Assignee: Boehringer Mannheim GmbH
    Inventors: Wolfgang Gruber, Hans U. Bergmeyer, Michael Nelbock-Hochstetter, Klaus Beaucamp, Gunter Holz, Johanna Gramsall, Gunter Lang
  • Process for the preparation of cholesterol oxidase
    Patent number: 4008127
    Abstract: Cholesterol oxidase which oxidizes cholesterol with oxygen to give cholestenone and hydrogen peroxide, is prepared by culturing Nocardia erthropolis ATCC 17895, Nocardia erthropolis ATCC 4277, Nocardia formica ATCC 14811 or Proactinomyce erythropolis NCIB 9158 first on a peptone-containing mineral salt medium and thereafter on at least one sterol compound as the sole source of carbon, and then isolating the cholesterol oxidase formed from the cultured micro-organism, wherein said sterol compound is of the formula ##STR1## in which R.sub.1 and R.sub.2 are hydrogen or together represent a double bond,R.sub.3 and R.sub.4 are hydrogen or together represent a double bond, andR.sub.5 is hydrogen or alkyl containing up to 3 carbon atoms.
    Type: Grant
    Filed: November 20, 1975
    Date of Patent: February 15, 1977
    Assignee: Boehringer Mannheim G.m.b.H.
    Inventors: Wolfgang Gruber, Hans Ulrich Bergmeyer, Michael Nelboeck-Hochstetter, Klaus Beaucamp, Gunter Holz, Johanna Gramsall, Gunter Lang