Patents by Inventor Johannes Adrianus Maria De Bont
Johannes Adrianus Maria De Bont has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11034979Abstract: The invention relates to fungal cells for the production of FDCA. The fungal cell is genetically modified to have at least one of a) a genetic modification that confers to or increases in the cell the ability to oxidize 5-hydroxymethyl-2-furancarboxylic acid to 5-formyl-2-furoic acid; and, b) a genetic modification that reduces catabolism of 2,5-furandicarboxylic acid in the cell. The fungal cell can further be genetically modified to increase the cell's ability to oxidize furanic aldehydes to the corresponding furanic carboxylic acids. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) wherein the cells of the invention are used for oxidation of a furanic precursors of FDCA.Type: GrantFiled: September 21, 2016Date of Patent: June 15, 2021Assignee: Purac Biochem B.V.Inventors: Johannes Adrianus Maria de Bont, Harald Johan Ruijssenaars, Jan Werij
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Patent number: 10941421Abstract: The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising, hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell.Type: GrantFiled: May 3, 2018Date of Patent: March 9, 2021Assignee: DSM IP ASSETS B.V.Inventors: Johannes Adrianus Maria De Bont, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Paul Klaassen, Wouter Willem Antonius Hartman, Shimaira Van Beusekom
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Publication number: 20200102584Abstract: The invention relates to fungal cells for the production of FDCA. The fungal cell has genetic modification that reduces specific 2,5-furandicarboxylic acid (FDCA) decarboxylating monooxygenase activity in the cell, as compared to a corresponding parent cell lacking the genetic modification. The fungal cell can further be genetically modified to increase the cell's ability to oxidize furanic aldehydes to the corresponding furanic carboxylic acids. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) wherein the cells of the invention are used for oxidation of a furanic precursors of FDCA to FDCA.Type: ApplicationFiled: March 21, 2018Publication date: April 2, 2020Applicant: Purac Biochem B.V.Inventors: Johannes Adrianus Maria de Bont, Harald Johan Ruijssenaars, Jan Werij
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Publication number: 20180371447Abstract: The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridium and Fusobacterium or a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.Type: ApplicationFiled: September 4, 2018Publication date: December 27, 2018Inventors: Aloysius Wilhelmus Rudolphus Hubertus TEUNISSEN, Johannes Adrianus Maria De Bont
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Patent number: 10093914Abstract: The present invention relates to eukaryotic cells which have the ability to isomerize xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridium and Fusobacterium or a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.Type: GrantFiled: April 7, 2016Date of Patent: October 9, 2018Assignee: DSM IP ASSETS B.V.Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
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Publication number: 20180265896Abstract: The invention relates to fungal cells for the production of FDCA. The fungal cell is genetically modified to have at least one of a) a genetic modification that confers to or increases in the cell the ability to oxidize 5-hydroxymethyl-2-furancarboxylic acid to 5-formyl-2-furoic acid; and, b) a genetic modification that reduces catabolism of 2,5-furandicarboxylic acid in the cell. The fungal cell can further be genetically modified to increase the cell's ability to oxidize furanic aldehydes to the corresponding furanic carboxylic acids. The invention also relates to a process for the production of 2,5-furan-dicarboxylic acid (FDCA) wherein the cells of the invention are used for oxidation of a furanic precursors of FDCA.Type: ApplicationFiled: September 21, 2016Publication date: September 20, 2018Applicant: Purac Biochem B.V.Inventors: Johannes Adrianus Maria de Bont, Harald Johan Ruijssenaars, Jan Werij
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Publication number: 20180251798Abstract: The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising, hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell.Type: ApplicationFiled: May 3, 2018Publication date: September 6, 2018Inventors: Johannes Adrianus Maria DE BONT, Aloysius TEUNISSEN, Paul KLAASSEN, Wouter Willem Antonius HARTMAN, Shimaira VAN BEUSEKOM
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Patent number: 9988649Abstract: The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterized in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell.Type: GrantFiled: November 26, 2012Date of Patent: June 5, 2018Assignee: DSM IP ASSETS B.V.Inventors: Johannes Adrianus Maria De Bont, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Paul Klaassen, Wouter Willem Antonius Hartman, Shimaira Van Beusekom
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Publication number: 20180030482Abstract: The instant invention relates to processes and systems for the fermentative production of ethanol. The ethanol is produced by fermenting a fermentable carbohydrate with a yeast, whereby acetaldehyde is externally supplied to the yeast cell for reducing glycerol by-product formation by the yeast cell, for improving the performance of yeast at high ethanol levels and/or for suppression of infections during the fermentation. The acetaldehyde that is externally supplied to the fermentation medium can be produced by catalytic oxidation of ethanol. Advantageously, acetaldehyde production from ethanol is integrated in a system for ethanol production. Thus, in another aspect the invention relates to a system for producing ethanol, e.g. an ethanol plant, which system, in addition to the usual means for fermentative production of ethanol, comprises a means for producing acetaldehyde by catalytic oxidation of ethanol.Type: ApplicationFiled: April 16, 2015Publication date: February 1, 2018Applicant: Moralco B.V.Inventor: Johannes Adrianus Maria de Bont
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Publication number: 20160230162Abstract: The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridium and Fusobacterium or a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.Type: ApplicationFiled: April 7, 2016Publication date: August 11, 2016Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
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Patent number: 9334488Abstract: The present invention relates to eukaryotic cells which have the ability to isomerize xylose directly into xylulose by transformation with nucleotide sequences encoding a xylose isomerase that has one or more specific sequence elements typical for isomerases which are functionally expressed in yeasts, such as xylose isomerases obtainable from bacteria of the genera Clostridium and Fusobacterium or a tunicate from the genus Ciona. The cell preferably is a yeast or a filamentous fungus capable of anaerobic alcoholic fermentation. The cells may further comprise one or more genetic modifications that increase the flux of the pentose phosphate pathway or, reduce unspecific aldose reductase activity. The cell preferably has the ability to produce a fermentation product such as ethanol, lactic acid, 3-hydroxy-propionic acid, ?-lactam antibiotics and cephalosporins. Also provided are processes for producing these fermentation products from xylose.Type: GrantFiled: December 24, 2009Date of Patent: May 10, 2016Assignee: DSM IP ASSETS B.V.Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
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Publication number: 20150184203Abstract: Eukaryotic cells, preferably a yeast or a filamentous fungus, with the ability to convert L-arabinose into D-xylulose 5-phosphate are provided, said ability acquired by transformation with nucleotide sequences coding for an arabinose isomerase, a ribulokinase, and a ribulose-5-P-4-epimerase from a bacterium of genus Clavibacter, Arthrobacter or Gramella. Preferably the can produce a fermentation product such as ethanol, lactic acid, 3-hydroxy-propionic acid, acrylic acid, acetic acid, succinic acid, citric acid, amino acids, 1,3-propane-diol, ethylene, glycerol, -lactam antibiotics and cephalosporins. The invention further relates to processes for producing these fermentation products wherein a cell of the invention is used to ferment arabinose into the fermentation products.Type: ApplicationFiled: February 17, 2015Publication date: July 2, 2015Inventor: Johannes Adrianus Maria DE BONT
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Publication number: 20150176032Abstract: The present invention relates to processes for producing ethanol from lignocellulosic hydrolysates comprising hexoses, pentoses and acetic acid, whereby genetically modified yeast cells are use that comprise an exogenous gene encoding an acetaldehyde dehydrogenase and a bacterial gene encoding an enzyme with NAD+-linked glycerol dehydrogenase activity. The process is further characterised in that glycerol is present in or fed into the culture medium, whereby the modified yeast cell ferments the hexoses, pentoses, acetic acid and glycerol to ethanol. The invention further relates to yeast cells for use in such processes. The yeast cells advantageously comprise genetic modifications that improve glycerol utilization such as modifications that increase one or more of dihydroxyacetone kinase activity and transport of glycerol into the cell.Type: ApplicationFiled: November 26, 2012Publication date: June 25, 2015Inventors: Johannes Adrianus Maria De Bont, Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Paul Klaassen, Wouter Willem Antonius Hartman, Shimaira Van Beusekom
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Publication number: 20110318790Abstract: The present invention relates to eukaryotic cells which have the ability to isomerise xylose directly into xylulose. The cells have acquired this ability by transformation with nucleotide sequences coding for a xylose isomerase that has one or more specific sequence elements typical for isomerases having the ability of functional expression in yeasts, such as e.g. xylose isomerases obtainable from a bacterium of the genera Clostridiumand Fusobacteriumor a tunicate form the genus Ciona. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation.Type: ApplicationFiled: December 24, 2009Publication date: December 29, 2011Inventors: Aloysius Wilhelmus Rudolphus Hubertus Teunissen, Johannes Adrianus Maria De Bont
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Publication number: 20100304454Abstract: The present invention relates to eukaryotic cells which have the ability to convert L-arabinose into D-xylulose 5-phosphate. The cells have acquired this ability by transformation with nucleotide sequences coding for an arabinose isomerase, a ribulokinase, and a ribulose-5-P-4-epimerase from a bacterium that belongs to a Clavibacter, Arthrobacter or Gramella genus. The cell preferably is a yeast or a filamentous fungus, more preferably a yeast is capable of anaerobic alcoholic fermentation. The may further comprise one or more genetic modifications that increase the flux of the pentose phosphate pathway, reduce unspecific aldose reductase activity, confer to the cell the ability to directly isomerise xylose into xylulose, increase the specific xylulose kinase activity, increase transport of at least one of xylose and arabinose into the host cell, decrease sensitivity to catabolite repression, increase tolerance to ethanol, osmolarity or organic acids; and/or reduce production of by-products.Type: ApplicationFiled: July 21, 2008Publication date: December 2, 2010Applicant: ROYAL NEDALCO B.V.Inventor: Johannes Adrianus Maria De Bont