Patents by Inventor Johannes Andries Roubos

Johannes Andries Roubos has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • GUIDE-RNA EXPRESSION SYSTEM FOR A HOST CELL
    Publication number: 20230287436
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a guide-RNA expression system for a eukaryotic host cell.
    Type: Application
    Filed: March 22, 2023
    Publication date: September 14, 2023
    Inventors: Eric Mosher YOUNG, Amar GHODASARA, René VERWAAL, Johannes Andries ROUBOS, Bianca Elisabeth Maria GIELESEN, Brenda VONK, Alec A.K. NIELSEN, Christopher Ashby VOIGT
  • LOW VOLUME TRANSFECTION
    Publication number: 20220389458
    Abstract: The invention relates to an improved method for the transfection of fungal cells.
    Type: Application
    Filed: November 2, 2020
    Publication date: December 8, 2022
    Inventors: Maarten Dirk VERHOEVEN, Johannes Andries ROUBOS, Noel Nicolaas Maria Elisabeth VAN PEIJ
  • CRISPR-CAS SYSTEM FOR AN ALGAL HOST CELL
    Publication number: 20220380759
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-Cas system for a Labyrinthulomycetes host cell.
    Type: Application
    Filed: August 5, 2022
    Publication date: December 1, 2022
    Inventors: James Casey LIPPMEIER, Yelena BETZ, Johannes Andries ROUBOS, Rene VERWAAL
  • CRISPR-Cas system for an algal host cell
    Patent number: 11466269
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-Cas system for a Labyrinthulomycetes host cell.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: October 11, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: James Casey Lippmeier, Yelena Betz, Johannes Andries Roubos, René Verwaal
  • MULTIPARTITE CRISPR DONOR
    Publication number: 20220235378
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-assembly gene editing system in a eukaryotic cell.
    Type: Application
    Filed: April 23, 2020
    Publication date: July 28, 2022
    Inventors: Brenda VONK, Adriana Christina VAN DER HOEK, Priscilla ZWARTJENS, Johannes Andries ROUBOS
  • CRISPR-CAS system for a filamentous fungal host cell
    Patent number: 11396665
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: July 26, 2022
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos, Carsten Pohl, Arnold Jacob Mattieu Driessen, Roelof Ary Lans Bovenberg, Yvonne Irene Nygård
  • CRISPR GUIDE-RNA EXPRESSION STRATEGIES FOR MULTIPLEX GENOME ENGINEERING
    Publication number: 20220056460
    Abstract: The invention relates to the field of molecular biology and cell biology. More specifically, the invention relates to CRISPR guide-RNA expression strategies for multiplex genome engineering.
    Type: Application
    Filed: November 29, 2019
    Publication date: February 24, 2022
    Inventors: Rene VERWAAL, Nathalie WIESSENHAAN, Johannes Andries ROUBOS
  • Assembly system for a eukaryotic cell
    Patent number: 11149268
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, René Verwaal, Bianca Elisabeth Maria Gielesen, Brenda Vonk
  • CRISPR-CAS system for a lipolytic yeast host cell
    Patent number: 11149288
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • CRISPR-CAS system for a yeast host cell
    Patent number: 11118193
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: September 14, 2021
    Assignee: DSM IP ASSESTS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • CRISPR TRANSIENT EXPRESSION CONSTRUCT (CTEC)
    Publication number: 20210071174
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR transient expression construct (CTEC) for a genome editing system.
    Type: Application
    Filed: May 6, 2019
    Publication date: March 11, 2021
    Inventors: Johannes Andries ROUBOS, Rene VERWAAL, Brenda VONK
  • Cloning method
    Patent number: 10865407
    Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via the cleavage sites, thereby to prepare two or more standardized expression cassettes.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: December 15, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, Herman Jan Pel, Bernard Meijrink
  • Recombination system
    Patent number: 10793850
    Abstract: The present invention relates to a method for carrying out recombination at a target locus.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: October 6, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Nathalie Wiessenhaan, Catharina Petronella Antonia Maria Kolen, Bernard Meijrink, Viktor Marius Boer, Johannes Andries Roubos, Yvonne Johannes Odilia Arendsen
  • A GUIDE-RNA EXPRESSION SYSTEM FOR A HOST CELL
    Publication number: 20200199599
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a guide-RNA expression system for a eukaryotic host cell.
    Type: Application
    Filed: July 17, 2017
    Publication date: June 25, 2020
    Inventors: Eric Mosher YOUNG, Amar GHODASARA, René VERWAAL, Johannes Andries ROUBOS, Bianca Elisabeth Maria GIELESEN, Brenda VONK, Alec A.K. NIELSEN, Christopher Ashby VOIGT
  • CRISPR-CAS SYSTEM FOR A YEAST HOST CELL
    Publication number: 20200172934
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: June 4, 2020
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS
  • CRISPR-CAS SYSTEM FOR A LIPOLYTIC YEAST HOST CELL
    Publication number: 20200165637
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: May 28, 2020
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS
  • CRISPR-CAS SYSTEM FOR AN ALGAL HOST CELL
    Publication number: 20200131511
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention trelates to a CRISPR-Cas system for a Labyrinthulomycetes host cell.
    Type: Application
    Filed: July 13, 2017
    Publication date: April 30, 2020
    Inventors: James Casey LIPPMEIER, Yelena BETZ, Johannes Andries ROUBOS, René VERWAAL
  • CRISPR-CAS system for a yeast host cell
    Patent number: 10619170
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: April 14, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • CRISPR-CAS system for a lipolytic yeast host cell
    Patent number: 10590436
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: March 17, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • SELF-GUIDING INTEGRATION CONSTRUCT (SGIC)
    Publication number: 20200032252
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a self-guiding integration construct for a genome editing system.
    Type: Application
    Filed: April 4, 2018
    Publication date: January 30, 2020
    Inventors: Johannes Andries ROUBOS, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Brenda VONK