Patents by Inventor Johannes Andries Roubos

Johannes Andries Roubos has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11149268
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Grant
    Filed: December 15, 2016
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, René Verwaal, Bianca Elisabeth Maria Gielesen, Brenda Vonk
  • Patent number: 11149288
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: October 19, 2021
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • Patent number: 11118193
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: February 3, 2020
    Date of Patent: September 14, 2021
    Assignee: DSM IP ASSESTS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • Publication number: 20210071174
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR transient expression construct (CTEC) for a genome editing system.
    Type: Application
    Filed: May 6, 2019
    Publication date: March 11, 2021
    Inventors: Johannes Andries ROUBOS, Rene VERWAAL, Brenda VONK
  • Patent number: 10865407
    Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via the cleavage sites, thereby to prepare two or more standardized expression cassettes.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: December 15, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Johannes Andries Roubos, Herman Jan Pel, Bernard Meijrink
  • Patent number: 10793850
    Abstract: The present invention relates to a method for carrying out recombination at a target locus.
    Type: Grant
    Filed: March 12, 2013
    Date of Patent: October 6, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Nathalie Wiessenhaan, Catharina Petronella Antonia Maria Kolen, Bernard Meijrink, Viktor Marius Boer, Johannes Andries Roubos, Yvonne Johannes Odilia Arendsen
  • Publication number: 20200199599
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a guide-RNA expression system for a eukaryotic host cell.
    Type: Application
    Filed: July 17, 2017
    Publication date: June 25, 2020
    Inventors: Eric Mosher YOUNG, Amar GHODASARA, René VERWAAL, Johannes Andries ROUBOS, Bianca Elisabeth Maria GIELESEN, Brenda VONK, Alec A.K. NIELSEN, Christopher Ashby VOIGT
  • Publication number: 20200172934
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: June 4, 2020
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS
  • Publication number: 20200165637
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: February 3, 2020
    Publication date: May 28, 2020
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS
  • Publication number: 20200131511
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention trelates to a CRISPR-Cas system for a Labyrinthulomycetes host cell.
    Type: Application
    Filed: July 13, 2017
    Publication date: April 30, 2020
    Inventors: James Casey LIPPMEIER, Yelena BETZ, Johannes Andries ROUBOS, René VERWAAL
  • Patent number: 10619170
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: April 14, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: René Verwaal, Bernard Meijrink, Nathalie Wiessenhaan, Brenda Vonk, Johannes Andries Roubos
  • Patent number: 10590436
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Grant
    Filed: January 6, 2016
    Date of Patent: March 17, 2020
    Assignee: DSM IP ASSETS B.V.
    Inventors: Bernard Meijrink, René Verwaal, Bianca Elisabeth Maria Gielesen, Johannes Andries Roubos
  • Publication number: 20200032252
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a self-guiding integration construct for a genome editing system.
    Type: Application
    Filed: April 4, 2018
    Publication date: January 30, 2020
    Inventors: Johannes Andries ROUBOS, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Brenda VONK
  • Publication number: 20190194692
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a filamentous fungal host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: June 27, 2019
    Inventors: Bernard MEIJRINK, René VERWAAL, Bianca Elisabeth Maria GIELESEN, Johannes Andries ROUBOS, Carsten POHL, Arnold Jacob Mattieu DRIESSEN, Roelof Ary Lans BOVENBERG, Yvonne Irene NYGÅRD
  • Publication number: 20190185843
    Abstract: The present invention is based on the advantageous use of single-stranded oligonucleotides in the in vivo (within a cell) assembly of double-stranded oligonucleotides into a single double-stranded nucleic acid construct. The present invention relates to the use of at least a first and a second single-stranded oligonucleotide in the assembly within a cell of at least two double-stranded nucleic acid molecules into a single double-stranded nucleic acid construct of pre-determined sequence, wherein the first and second single-stranded oligonucleotide are essentially complementary to each other.
    Type: Application
    Filed: December 15, 2016
    Publication date: June 20, 2019
    Inventors: Johannes Andries ROUBOS, René VERWAAL, Blance Elisabeth Maria GIELESEN, Brenda VONK
  • Publication number: 20190055544
    Abstract: The present invention relates relates to a CRISPR-CAS system for a host cell, in particular to a method to produce a circular vector comprising one or more guide-polynucleotide expression cassettes, wherein said one or more guide-polynucleotide expression cassettes comprise a polynucleotide encoding a guide-polynucleotide operably linked to one or more control sequences which direct the expression of said guide-polynucleotide in a host cell, wherein said guide-polynucleotide comprises a guide-sequence that essentially is the reverse complement of a target-polynucleotide in a host cell and wherein the guide-polynucleotide can direct binding of a Cas protein at a target-polynucleotide in a host cell to form a CRISPR-Cas complex, in vivo.
    Type: Application
    Filed: July 6, 2016
    Publication date: February 21, 2019
    Inventors: Paulus Petrus DE WAAL, Johannes Andries ROUBOS, Paul KLAASSEN, René VERWAAL
  • Patent number: 9951339
    Abstract: The invention relates to a method for modification of a host cell at a target locus, which method comprises: providing a host cell comprising, at a first locus, at least two site-specific recombination sites and a nucleic acid having an essential function or encoding a product having an essential function; introducing into the host cell, at the target locus, a further nucleic acid having the essential function or encoding for a product having the essential function; and carrying out recombination at the first locus via the at least two site-specific recombination sites, so that the nucleic acid having an essential function or encoding a product having an essential function is rendered non-functional, thereby to modify the host cell at the target locus. The invention also relates to a cell obtainable by a method of the invention.
    Type: Grant
    Filed: September 19, 2013
    Date of Patent: April 24, 2018
    Assignee: DSM IP ASSETS B.V.
    Inventors: Ilse De Lange, Bernard Meijrink, Johannes Andries Roubos, Siew-loon Ooi
  • Publication number: 20180023096
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a lipolytic yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 25, 2018
    Applicant: DSM IP ASSETS B.V.
    Inventors: BERNARD MEIJRINK, RENÉ VERWAAL, BIANCA ELISABETH MARIA GIELESEN, JOHANNES ANDRIES ROUBOS
  • Publication number: 20180010151
    Abstract: The present invention relates to the field of molecular biology and cell biology. More specifically, the present invention relates to a CRISPR-CAS system for a yeast host cell.
    Type: Application
    Filed: January 6, 2016
    Publication date: January 11, 2018
    Inventors: René VERWAAL, Bernard MEIJRINK, Nathalie WIESSENHAAN, Brenda VONK, Johannes Andries ROUBOS
  • Publication number: 20170314011
    Abstract: The present invention relates to a method based on the use of restriction enzyme digestion and ligation via the cleavage sites, thereby to prepare two or more standardized expression cassettes.
    Type: Application
    Filed: July 13, 2017
    Publication date: November 2, 2017
    Inventors: Johannes Andries ROUBOS, Herman Jan Pel, Bernard Meijrink