Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a promoter sequence and a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified SEQ ID NO: 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO: 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Abstract: In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.

Abstract: The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.

Abstract: In a washing or cleaning agent that contains a solid enzyme formulation, the intention is to improve shelf stability in terms of enzymatic activity. This is achieved by a washing or cleaning agent comprising an enzyme granulate, the granulate comprising, besides the enzyme, the following components: (a) 68 to 90 wt % (w/w) sulfate, in particular alkali metal sulfate, particularly preferably sodium sulfate, (b) 0.1 to 10.5 wt % (w/w) polyethylene glycol, (c) 0.5 to 14.5 wt % (w/w) granulation adjuvant.

Abstract: In a microbial fermentation, the aim is to increase the product yield of protein. This is achieved by a method in which an expression construct is introduced into a microorganism of the species Bacillus pumilus which comprises a promoter and a nucleic acid coding for the protein, and the protein is expressed in said expression construct.

Abstract: The aim of the invention is to improve the secretion of a protein from a host cell in order to increase the product yield of protein in a fermentation process. This is achieved by an expression vector comprising a) a promoter sequence and b) a nucleic acid sequence that codes for a protein. The protein comprises a signal peptide and an additional amino acid sequence, and the signal peptide comprises an amino acid sequence that is at least 80% identical to the amino acid sequence specified in SEQ ID NO 2, at least 80% identical to the amino acid sequence specified in SEQ ID NO 4, at least 80% identical to the amino acid sequence specified in SEQ ID NO 6, or the signal peptide comprises an amino acid sequence that is structurally homologous to at least one of said sequences.

Abstract: The aim is to increase the protein product yield in microbial fermentation. This is achieved by a method which introduces into a microorganism not only a first expression construct which encodes the protein, but also a second expression construct which encodes an auxiliary protease which differs from the protein, is proteolytically active and which comprises an amino acid sequence which is at least 50% identical to the amino acid sequence indicated in SEQ ID NO. 1.

Abstract: In a washing or cleaning agent that contains a solid enzyme formulation, the intention is to improve shelf stability in terms of enzymatic activity. This is achieved by a washing or cleaning agent comprising an enzyme granulate, the granulate comprising, besides the enzyme, the following components: (a) 68 to 90 wt % (w/w) sulfate, in particular alkali metal sulfate, particularly preferably sodium sulfate, (b) 0.1 to 10.5 wt % (w/w) polyethylene glycol, (c) 0.5 to 14.5 wt % (w/w) granulation adjuvant.

Abstract: The present invention relates to a method for increasing the cleaning performance of a washing or cleaning agent comprising at least one hydrolytic enzyme by addition of a component capable of synergistic interaction with the enzyme. Components capable of such positive synergistic interaction with hydrolytic enzymes include: (i) an amino acid, a polyamino acid, or derivatives thereof; (ii) a biosurfactant; (iii) a microbial metabolite, and a preparation of a microbial culture supernatant that contains at least 2.5 wt % of (i), (ii), or (iii). Additionally, the present invention comprises a method for washing textiles or hard surfaces with an enzymatic washing or cleaning agent that includes at least one of the components (i), (ii), or (iii) capable of increasing the cleaning performance of the washing or cleaning agent through synergistic interaction with the hydrolytic enzyme.

Abstract: Proteins having a cofactor can be secreted in an improved manner in a microorganism belonging to the genus Streptomyces provided that the microorganism contains a nucleic acid sequence which is not naturally present in it and which comprises at least the following sequence sections: a) nucleic acid sequence coding for a protein containing a cofactor, and b) a nucleic acid sequence which is at least 20% identical to the sequence given in SEQ ID NO. 1, or at least 20% identical to the sequence given in SEQ ID NO. 3, or a nucleic acid sequence which is structurally homologous to at least one of these sequences, wherein the amino acid sequence encoded by nucleic acid sequence b) functionally cooperates with the amino acid sequence encoded by nucleic acid sequence a) so that at least the amino acid sequence encoded by nucleic acid sequence a) is secreted by the microorganism.

Abstract: Proteins having a cofactor can be secreted in an improved manner in a microorganism belonging to the genus Corynebacterium provided that the microorganism contains a nucleic acid sequence which is not naturally present in it and which comprises at least the following sequence sections: a) nucleic acid sequence coding for a protein which contains a cofactor, and b) a nucleic acid sequence which is at least 20% identical to the sequence given in SEQ ID NO. 1 or a nucleic acid sequence which is a structural homologue to this sequence, wherein the amino acid sequence which is encoded by the nucleic acid sequence b) functionally interacts with the amino acid sequence encoded by the nucleic acid sequence a) in such a manner that at least the amino acid sequence encoded by the nucleic acid sequence a) is excreted by the microorganism.

Abstract: A process for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway The invention relates to a process for the microbial production of aromatic amino acids and other metabolites of the aromatic amino acid biosynthetic pathway. Microbially produced substances such as fine chemicals, in particular aromatic amino acids or metabolites of the aromatics biosynthetic pathway, are of great economic interest, and the need for amino acids, for example, continues to increase. The inventors found that, after introducing a pyc gene sequence into microorganisms, it was possible to produce aromatic amino acids and also metabolites of the aromatic biosynthetic pathway in an improved manner. The process of the invention is particularly suitable for producing L-phenylalanine.