Patents by Inventor Johannes C. Clevers
Johannes C. Clevers has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11035852Abstract: The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.Type: GrantFiled: June 8, 2018Date of Patent: June 15, 2021Assignees: UMC Utrecht Holding, B.V., Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeffrey Matthijn Beekman, Johanna Florentia Dekkers, Cornelis Korstiaan Van Der Ent, Johannes C. Clevers
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Patent number: 10947510Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising said epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of said culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in said culture medium.Type: GrantFiled: November 13, 2013Date of Patent: March 16, 2021Assignee: Koninklijke Nederlandse Akademie Van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Patent number: 10940180Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: GrantFiled: November 15, 2017Date of Patent: March 9, 2021Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Publication number: 20190383799Abstract: The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.Type: ApplicationFiled: June 8, 2018Publication date: December 19, 2019Applicants: UMC Utrecht Holding B.V., Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeffrey Matthijn Beekman, Johanna Florentia Dekkers, Cornelis Korstiaan Van Der Ent, Johannes C. Clevers
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Publication number: 20190100728Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in the culture medium.Type: ApplicationFiled: August 27, 2018Publication date: April 4, 2019Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Publication number: 20180221441Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: ApplicationFiled: November 15, 2017Publication date: August 9, 2018Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Patent number: 10006904Abstract: The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.Type: GrantFiled: December 19, 2012Date of Patent: June 26, 2018Assignees: UMC Utrecht Holding B.V., Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeffrey Matthijn Beekman, Johanna Florentia Dekkers, Cornelis Korstiaan Van Der Ent, Johannes C. Clevers
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Publication number: 20180072995Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids, pancreatic organoids, liver organoids, colon organoids, Barrett's Esophagus organoids, adenocarcinoma organoids and colon carcinoma organoids that are formed in the culture medium.Type: ApplicationFiled: July 19, 2017Publication date: March 15, 2018Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Patent number: 9833496Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: GrantFiled: September 23, 2014Date of Patent: December 5, 2017Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Patent number: 9752124Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids, pancreatic organoids, liver organoids, colon organoids, Barrett's Esophagus organoids, adenocarcinoma organoids and colon carcinoma organoids that are formed in the culture medium.Type: GrantFiled: July 29, 2011Date of Patent: September 5, 2017Assignee: Koninklijke Nederlandse Akademie van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Publication number: 20170151308Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: ApplicationFiled: September 23, 2014Publication date: June 1, 2017Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Publication number: 20150276719Abstract: The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.Type: ApplicationFiled: December 19, 2012Publication date: October 1, 2015Applicants: UMC Utrecht Holding B.V., Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeffrey Matthijn Beekman, Johanna Florentia Dekkers, Cornelis Korstiaan Van Der Ent, Johannes C. Clevers
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Publication number: 20150231201Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: ApplicationFiled: September 23, 2014Publication date: August 20, 2015Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus Lambertus Van de Wetering
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Publication number: 20150011420Abstract: The invention relates to an assay for diagnosing a disease or affliction that affects fluid uptake or secretion or for studying the effectiveness of one or more drugs for treating the disease or affliction, wherein the assay comprises measuring swelling of one or more organoids.Type: ApplicationFiled: December 19, 2012Publication date: January 8, 2015Applicants: UMC Utrecht Holding B.V., Koninklijke Nederlandse Akademie van WetenschappenInventors: Jeffrey Matthijn Beekman, Johanna Florentia Dekkers, Cornelis Korstiaan Van Der Ent, Johannes C. Clevers
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Publication number: 20140256037Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising said epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of said culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in said culture medium.Type: ApplicationFiled: November 13, 2013Publication date: September 11, 2014Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Publication number: 20140243227Abstract: Culture media and methods for expanding and differentiating populations of stem cells and for obtaining organoids. Expanded cell populations and organoids obtainable by methods of the invention and their use in drug screening, toxicity assays and regenerative medicine.Type: ApplicationFiled: June 11, 2012Publication date: August 28, 2014Applicant: Koninklijke Nederlandse Akademie van WetenschappenInventors: Johannes C. Clevers, Toshiro Sato, Meritxell Huch Ortega, Wouter Richard
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Publication number: 20120196312Abstract: The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids, pancreatic organoids, liver organoids, colon organoids, Barrett's Esophagus organoids, adenocarcinoma organoids and colon carcinoma organoids that are formed in the culture medium.Type: ApplicationFiled: July 29, 2011Publication date: August 2, 2012Inventors: Toshiro Sato, Johannes C. Clevers, Meritxell Huch Ortega
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Publication number: 20100275280Abstract: The invention relates to the fields of biochemistry, pharmacy and oncology. The invention particularly relates to the use of novel stem cell markers for the isolation of stem cells. The invention further relates to the obtained stem cells and their use in for example research or treatment, for example, for the preparation of a medicament for the treatment of damaged or diseased tissue. In one of the embodiments, the invention provides a method for obtaining (or isolating) stem cells comprising optionally preparing a cell suspension from a tissue or organ sample, contacting said cell suspension with an Lgr 6 or 5 binding compound, identify the cells bound to said binding compound, and optionally isolating the stem cells from said binding compound. The invention further relates to means suitable for cancer treatment and even more specific for the treatment of cancer by eradicating cancer stem cells.Type: ApplicationFiled: May 10, 2010Publication date: October 28, 2010Applicants: Hubrecht Institute, Konicklijke Nederlandse Akademie Van WetenschappenInventors: Johannes C. Clevers, Nicholas Barker, Andrea Haegebarth, Marcus L. Van De Wetering