Abstract: A method for the in situ derivatization of at least one biogenic amine, precursor, or metabolite thereof in an isolated aqueous sample includes the steps of: (i) contacting the sample with a propionic anhydride/acetonitrile solution in the presence of a phosphate buffer having a pH in the range of 7.0 to 9.0 and allowing the conversion of amine and/or hydroxyl moieties of the biogenic amine, precursor, or metabolite thereof to form a propionyl derivative of the biogenic amine; followed by (ii) adding to the reaction mixture obtained in step (i) a carbodiimide compound and an electrophilic amine-containing compound, and allowing the carbodiimide-mediated derivatization of carboxylic acid moieties of the biogenic amine, precursor, or metabolite thereof.

Abstract: A method for the in situ derivatization of at least one biogenic amine, precursor, or metabolite thereof in an isolated aqueous sample includes the steps of: (i) contacting the sample with a propionic anhydride/acetonitrile solution in the presence of a phosphate buffer having a pH in the range of 7.0 to 9.0 and allowing the conversion of amine and/or hydroxyl moieties of the biogenic amine, precursor, or metabolite thereof to form a propionyl derivative of the biogenic amine; followed by (ii) adding to the reaction mixture obtained in step (i) a carbodiimide compound and an electrophilic amine-containing compound, and allowing the carbodiimide-mediated derivatization of carboxylic acid moieties of the biogenic amine, precursor, or metabolite thereof.

Abstract: A method for the in situ derivatization of at least one biogenic amine, precursor, or metabolite thereof in an isolated aqueous sample includes the steps of: (i) contacting the sample with a propionic anhydride/acetonitrile solution in the presence of a phosphate buffer having a pH in the range of 7.0 to 9.0 and allowing the conversion of amine and/or hydroxyl moieties of the biogenic amine, precursor, or metabolite thereof to form a propionyl derivative of the biogenic amine; followed by (ii) adding to the reaction mixture obtained in step (i) a carbodiimide compound and an electrophilic amine-containing compound, and allowing the carbodiimide-mediated derivatization of carboxylic acid moieties of the biogenic amine, precursor, or metabolite thereof.

Abstract: The invention relates to analytical chemistry, in particular to means and methods for the quantitative determination of derivatized biogenic amines, precursors or metabolites thereof. Provided is a method for the in situ derivatization of at least one biogenic amine, precursor or metabolite thereof in an isolated aqueous sample, the derivatization comprising the steps of: (i) contacting said sample with a propionic anhydride/acetonitril solution in the presence of a phosphate buffer having a pH in the range of 7.0 to 9.0 and allowing the conversion of amine and/or hydroxyl moieties of said biogenic amine or metabolite thereof to form a propionyl derivative of said biogenic amine; followed by (ii) adding to the reaction mixture obtained in step (i) a carbodiimide compound and an electrophilic amine-containing compound, and allowing the carbodiimide-mediated derivatization of carboxylic acid moieties of a biogenic amine, precursor or metabolite thereof.

Abstract: The invention relates to the field of diagnostic methods, in particular to detection of biological molecules using mass spectroscopy (MS). Provided is an automated high-throughput method for quantitating a low molecular weight protein-bound biomarker directly in an isolated biological sample, comprising the steps of: (a) automated prepurification of the sample using an effective amount of an acid protease under conditions that allow for digestion of one or more binding proteins; (b) applying said protease-digested sample onto an on-line SPE column to capture at least part of said biomarker, followed by sequential washing of the solid phase; (c) eluting a fraction comprising said biomarker directly onto a liquid chromatography (LC) column comprising an apolar stationary phase and subjecting it to LC-MS or LC-MS-MS measurements to determine the amount of at least one biomarker; and (d) quantitating the biomarker(s).

Abstract: The present invention relates to a method to prepare a polymer dispersion using an aqueous substantially surfactant-free emulsion polymerization process comprising a seed and a feed stage, in which seed stage at least one ethylenically unsaturated monomer, having a water-solubility of at least 0.3 g/l at polymerization conditions, is polymerized in the presence of an addition fragmentation chain transfer agent and a hydrophilic free radical initiator to form a seed polymer that is substantially insoluble in water and in which feed stage at least one ethylenically unsaturated feed monomer is added to the seed polymer to form polymer particles. With this method a polymer dispersion can be obtained having at least 25 wt. % solid contents in combination with an average polymer particle size smaller than or equal to 300 nm.

Abstract: The invention relates to a combustion engine (1) having a housing (2) with a chamber (3). A rotor (4) is arranged herein which is provided with a number of vanes (5A, 5B, 6A, 6B) which divide the chamber into a number of compartments (3A, 3B, 3C, 3D). Each of the compartments is intended for performing at least one of the following functions: a) drawing in and/or compressing gas required for the combustion; b) bringing the fuel to combustion; c) producing work; and d) discharging combustion gases. A first pair of vanes (5A, 5B) is mounted rotatably on a first rotation axis (5). A second pair of vanes (6A, 6B) is mounted rotatably on a second rotation axis (6). The rotation axes are arranged eccentrically in the chamber (3). The rotary engine has the characteristic that the vanes in each pair (5A, 5B; 6A, 6B) are rotatable independently of each other.

Abstract: The invention relates to a combustin engine (1) having a housing (2) with a chamber (3). A rotor (4) is arranged herein which is provided with a number of vanes (5A, 5B, 6A, 6B) which divide the chamber into a number of compartments (3A, 3B, 3C, 3D). Each of the compartments is intended for performing at least one of the following functions: a) drawing in and/or compressing gas required for the combustion; b) bringing the fuel to combustion; c) producing work; and d) discharging combustion gases. A first pair of vanes (5A, 5B) is mounted rotatably on a first rotation axis (5). A second pair of vanes (6A, 6B) is mounted rotatably on a second rotation axis (6). The rotation axes are arranged eccentrically in the chamber (3). The rotary engine has the characteristic that the vanes in each pair (5A, 5B; 6A, 6B) are rotatable independently of each other.

Abstract: The invention pertains to a polymer obtainable from the steps: polymerizing a mixture, that is substantially free or organic solvents, of ethylenically unsaturated monomers which consist for at least 70% of methacrylates, and which comprises specific chain transfer agents, ?-methylstyrene dimer being preferred, to form a first polymer, aqueous emulsion polymerizing a mixture comprising ethylenically unsaturated monomers in the presence of the first polymer to form a dispersion of a water-insoluble second polymer, wherein these steps are preferably performed in the absence or the substantial absence of a surfactant.

Abstract: The invention pertains to a polymer obtainable from the steps:

Abstract: The invention pertains to water-dissipatable polymers comprising lipophilic polymers having pendant sulfonic acid salt groups and, optionally, pendant nonionic groups, methods of making such water-dissipatable polymers and the use of such polymers in aqueous systems, including aqueous coating compositions. The invention also pertains to addition polymer polyols having such pendant sulfonic acid salt groups and, optionally, pendant nonionic groups, their methods of manufacture and their use in aqueous systems, including aqueous coating compositions. The polymers of the invention may be used as, for example, binders, emulsifying agents and dispersing agents in aqueous systems, especially aqueous coating compositions.

Abstract: The invention pertains to water-dissipatable polymers comprising lipophilic polymers having pendant sulfonic acid salt groups and, optionally, pendant nonionic groups, methods of making such water-dissipatable polymers and the use of such polymers in aqueous systems, including aqueous coating compositions. The invention also pertains to addition polymer polyols having such pendant sulfonic acid salt groups and, optionally, pendant nonionic groups, their methods of manufacture and their use in aqueous systems, including aqueous coating compositions. The polymers of the invention may be used as, for example, binders, emulsifying agents and dispersing agents in aqueous systems, especially aqueous coating compositions.