Abstract: A microfluidic device (100) comprising at least one passive valve (PV, 120, 130), the passive valve being constituted by a sequence of a first carrier layer (CL 1) with an aperture providing a passage (P 1) for fluid flow, a first binding layer (BLI) with a first opening, a flexible layer (FL) with a through-hole (TH), a second binding layer (BL2) with a second opening, and a second carrier layer (CL2). Moreover, the flexible layer (FL) can move within a valve chamber (VC) constituted by the openings. Depending on the pressure difference across the passive valve (PV), the flexible layer (FL) can bend towards the first carrier layer (CLI) and close the aperture, while it opens the passage (P1) when bending in the opposite direction. Two of such properly oriented passive valves together with an intermediate active valve (AV) can constitute a one-stroke pump. A method for manufacturing such a fluidic device is also disclosed.