Abstract: Disclosed are methods and systems to detect a class of non-variola Orthopoxviruses, and, more particularly, monkeypox virus. In certain embodiments, the method may comprise obtaining a sample from the subject; and detecting a nucleic acid sequence specific to the non-variola Orthopoxvirus. In some embodiments, the method may further isolation of the non-variola Orthopoxvirus from a solid substrate such as a tissue swab and further purification of viral nucleic acid using silica beads. The sample may in some cases be self-collected by a subject. The disclosed methods and systems, including kits for self-collection, may be used for a high throughput assay allowing for screening of hundreds of samples per day.

Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

Abstract: The present invention provides novel methods and devices that employ microfluidic technology to generate molecular melt curves. In particular, the devices and methods in accordance with the invention are useful in providing for the analysis of PCR amplification products.

Abstract: The invention provides novel SGP primers for improved use in waveform-profiling methods of DNA amplification. In one embodiment, use of an SGP primer in a method of DNA amplification results in exponential amplification of several distinct products. In another embodiment, the methods of the invention further comprise a novel half-time elongation step. In another embodiment, the distinct products may be detected via melting temperature analysis. The primers and methods of the invention may be combined to determine an organism in a sample.

Abstract: A colorimeter capable of calibrating color monitors, whether having cathode ray tube or liquid crystal (LCD) displays, is provided by a photometric array of photodetector and optical filter pairs. The filters include long-pass, edge filters which cover overlapping regions at the upper end of the visible spectrum and a filter which covers the entire visible spectrum. The outputs of the photodetectors are digitally synthesized to provide a response which mimics the response established by the Commission Internationale de 1′Eclairage (CIE) xyz (bar) functions almost perfectly. The response which is mimicked may be represented by the CIE color matching functions. The pairs and the associated components are mounted on a printed circuit board captured in a clamshell housing and having an array of apertures which define angularly constrained fields of view of a surface from which the light, to be colormetrically analyzed, emanates.

Abstract: A colorimeter capable of calibrating color monitors, whether having cathode ray tube or liquid crystal (LCD) displays, is provided by a photometric array of photodetector and optical filter pairs. The filters include long-pass, edge filters which cover overlapping regions at the upper end of the visible spectrum and a filter which covers the entire visible spectrum. The outputs of the photodetectors are digitally synthesized to provide a response which mimics the response established by the Commission Internationale de l'Eclairage (CIE) xyz (bar) functions almost perfectly. The response which is mimicked may be represented by the CIE color matching functions. The pairs and the associated components are mounted on a printed circuit board captured in a clamshell housing and having an array of apertures which define angularly constrained fields of view of a surface from which the light, to be colormetrically analyzed, emanates.

Abstract: A unitary hand-held bar code scanner and reader produces an elliptical beam, oriented with its major axis along the direction of the bars, utilizing optics employing far field diffraction effects to shape the beam and maintain its elliptical aspect (length to width ratio) constant over a distance in front of the scanner were bar codes may be located. The optics eliminates parallax even though the photodetector and light source (preferably a laser diode) are located offset from each other on a board on which the optics are mounted. A housing assembly has channels which mount the board therein without shock absorbing devices. A digital microcomputer controller and peripheral devices regulate the optical power output from the laser diode and prevents catastrophic failure, if the electrical current through the laser diode exceeds safe limits.

Abstract: A unitary hand-held bar code scanner and reader produces an elliptical beam, oriented with its major axis along the direction of the bars, utilizing optics employing far field diffraction effects to shape the beam and maintain its elliptical aspect (length to width ratio) constant over a distance in front of the scanner were bar codes may be located. The optics eliminates parallax even though the photodetector and light source (preferably a laser diode) are located offset from each other on a board on which the optics are mounted. A housing assembly has channels which mount the board therein without shock absorbing devices. A digital microcomputer controller and peripheral devices regulate the optical power output from the laser diode and prevents catastrophic failure, if the electrical current through the laser diode exceeds safe limits.

Abstract: A unitary hand-held bar code scanner and reader produces an elliptical beam, oriented with its major axis along the direction of the bars, utilizing optics employing far field diffraction effects to shape the beam and maintain its elliptical aspect (length to width ratio) constant over a distance in front of the scanner were bar codes may be located. The optics eliminates parallax even though the photodetector and light source (preferably a laser diode) are located offset from each other on a board on which the optics are mounted. A housing assembly has channels which mount the board therein without shock absorbing devices. A digital microcomputer controller and peripheral devices regulate the optical power output from the laser diode and prevents catastrophic failure, if the electrical current through the laser diode exceeds safe limits.

Abstract: A bar code scanner has a laser which produces a beam incident on a deflector, such as a polygon, which projects a scan beam out of the scanner so as to scan a bar code; light reflected or scattered from the code being detected so as to provide signals from which the bar code may be decoded. In order to insure that the scan beam is incident on the code, a bright oblong spot is provided by a second laser and a mirror which deflects the beam from the second laser along a path, generally paralleling the scan beam, through a cylindrical lens, and intersecting the scan beam approximately in the center of the scan on the code. The laser producing the aiming spot and the deflector drive circuits are initiated upon actuation of a trigger.

Abstract: To increase the efficiency of personnel conducting inventory management operations, including data entry of products and information as to their absence, presence or location, the operator is provided with a glove having finger sheaths and a portion for the posterior or dorsal surface of the hand which covers that surface. A bar code reader is located in a housing on the cover and flexural strain gauge elements are located in the sheaths in the vicinity of the joints of the fingers. Signals from these elements are digitized and provide for manual data entry and also for commands to operate the bar code reader. The glove and the sensors constitute a portable transaction or data entry terminal which does not require manual actuation of a trigger to operate the bar code scanner or a keyboard for manual entry of data concerning the products to be managed.

Abstract: A headset to be worn around the head of an operator having a head-band, a microphone, a bar code reader, a display, electronics including a microprocessor and speech recognition for translating alphanumeric names and words into data which are to be stored in the memory of the microprocessor and a transceiver for exchanging data with a remotely located modem. Upon a voice activation, messages issued by the microprocessor are visually projected on a display and the reader is initiated.

Abstract: A unitary hand-held bar code scanner and reader produces an elliptical beam, oriented with its major axis along the direction of the bars, utilizing optics employing far field diffraction effects to shape the beam and maintain its elliptical aspect (length to width ratio) constant over a distance in front of the scanner were bar codes may be located. The optics eliminates parallax even though the photodetector and light source (preferably a laser diode) are located offset from each other on a board on which the optics are mounted. A housing assembly has channels which mount the board therein without shock absorbing devices. A digital microcomputer controller and peripheral devices regulate the optical power output from the laser diode and prevents catastrophic failure, if the electrical current through the laser diode exceeds safe limits.

Abstract: Miniature scan modules which occupy a volume of less than about 1 cubic inch so as to facilitate installation in any apparatus which requires scanning of a light beam across a target and detection of light reflected from the target. The miniature size of the scan module enables it to be incorporated in various apparatus, such as keyboards or terminals and provide them with the ability to read various symbols, particularly bar codes. The module has at least two printed circuit boards on at least one of which a source of a light beam, such as a laser diode or light emitting diode is mounted and another board which may be fixed in the apparatus in which the module is installed. The boards are interconnected by structure including a flexure which defines a pivot axis for the light source and the board on which it is mounted. The flexure is made up of a plurality of elements which are insulated from each other and provide separate conductive paths for electrical power and signals between the boards.

Abstract: An improved bar code scanning and reading apparatus which is adapted to be hand-held utilizes anamorphic optics for scanning and reading the code by providing a beam having an oblong cross-section in the nominal plane of the code. The oblong cross-section may be aligned with the bars of the code. The optics does not focus the beam in the longitudinal direction of the cross-section at the nominal plane of the code so that diffraction effects which would otherwise cause rotation of the cross-section of the beam to a position transverse to the bars of the code which would preclude reading of narrow bars, is avoided. The last lens of the anamorphic system may be a cylindrical lens which provides a line focus of a visible marker beam while at the same time providing the oblong cross-section of the beam which scans the code. The light from the vicinity of the code passes through the cylindrical lens and is collected by a toric reflector which projects the incoming light onto a photodetector.

Abstract: A bar code scanner having a housing in which a laser diode and optics forming the beam which scans the code and a photodetector are assembled as a unitary structure upon a printed circuit board so that the entire structure can be located in the housing.

Abstract: In order to meet the requirements for laser radiation safety, namely laser output which can enter the naked human eye indefinitely and cause no discernible damage (Class I emission limit) in a code symbol (bar code) scanner, a mode controller is used for generating a laser enabling the signal so as to turn the laser on in a search mode until the symbols are acquired and in an in-code mode while the symbols are being scanned. When the laser is turned on it operates at a power output which would exceed the Class I emission limits if operated continuously. Circuitry is provided for operating the mode controller so that the laser is not enabled to turn on for a period long enough to exceed Class I limits. Such circuitry may be an integrator or accumulator which responds to the time that the mode controller turns the laser on and which provides an output for inhibiting the mode controller from turning the laser on for a period of time sufficient to prevent the Class I emission limit from being exceeded.

Abstract: An optical device for detecting bar codes (markings or patterns which symbolize data such as the name and vendor of a product) are detected by an optical device which serves as a bar code scanner. A laser diode and associated optics provides an invisible beam. A visible marker beam coincident with the laser beam is generated by a lamp and associated optics and directed coaxial with the laser beam so as to enable the laser beam to be scanned across the bar code by moving a housing in which the laser and the lamp and their associated optics are disposed. Also contained in the housing is a photodetector on which scattered light from the code is incident after passing through a collection lens located at an outlet port for the beams. An aperture is positioned between the collection lens and the detector.