Patents by Inventor John A. Bridgham
John A. Bridgham has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 11795503Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: GrantFiled: July 15, 2019Date of Patent: October 24, 2023Assignee: Life Technologies CorporationInventors: George A. Fry, Christina E. Inman, John Bridgham, Timothy Hunkapiller, Charles S. Vann
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Publication number: 20190338351Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: ApplicationFiled: July 15, 2019Publication date: November 7, 2019Inventors: George A. FRY, Christina E. INMAN, John BRIDGHAM, Timothy HUNKAPILLER, Charles S. VANN
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Publication number: 20190233887Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.Type: ApplicationFiled: December 19, 2018Publication date: August 1, 2019Inventors: Jon A. HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David M. COX, Kirk M. HIRANO, John BRIDGHAM, George Stefan GOLDA, Sam Lee WOO
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Patent number: 10351907Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: GrantFiled: October 16, 2014Date of Patent: July 16, 2019Assignee: LIFE TECHNOLOGIES CORPORATIONInventors: George A. Fry, Christina E. Inman, John Bridgham, Timothy Hunkapiller, Charles S. Vann
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Patent number: 9273354Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.Type: GrantFiled: April 4, 2014Date of Patent: March 1, 2016Assignee: Illumina, Inc.Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
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Publication number: 20150087530Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.Type: ApplicationFiled: September 26, 2014Publication date: March 26, 2015Inventors: Jon A. HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David M. COX, Kirk M. HIRANO, John BRIDGHAM, George Stefan GOLDA, Sam Lee WOO
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Publication number: 20150065356Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: ApplicationFiled: October 16, 2014Publication date: March 5, 2015Inventors: George A. FRY, Christina E. INMAN, John BRIDGHAM, Timothy HUNKAPILLER, Charles S. VANN
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Patent number: 8889596Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: GrantFiled: August 16, 2013Date of Patent: November 18, 2014Assignee: Life Technologies CorporationInventors: George A. Fry, Christina E. Inman, John Bridgham, Timothy Hunkapiller, Charles S. Vann
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Publication number: 20140213464Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.Type: ApplicationFiled: April 4, 2014Publication date: July 31, 2014Applicant: ILLUMINA, INC.Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
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Patent number: 8728729Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.Type: GrantFiled: December 4, 2012Date of Patent: May 20, 2014Assignee: Illumina, Inc.Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
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Publication number: 20130331276Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: ApplicationFiled: August 16, 2013Publication date: December 12, 2013Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Mark F. OLDHAM, George A. FRY, Christina E. INMAN, John BRIDGHAM, Timothy HUNKAPILLER, Charles S. VANN
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Patent number: 8536099Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: GrantFiled: August 31, 2010Date of Patent: September 17, 2013Assignee: Life Technologies CorporationInventors: Mark Oldham, George Fry, Christina Inman, John Bridgham, Timothy Hunkapillar, Charles Vann
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Patent number: 8361713Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.Type: GrantFiled: October 12, 2007Date of Patent: January 29, 2013Assignee: Illumina, Inc.Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
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Publication number: 20110136677Abstract: According to various embodiments, a method is provided that comprises washing an array of DNA-coated beads on a substrate, with a wash solution to remove stacked beads from the substrate. The wash solution can include inert solid beads in a carrier. The DNA-coated beads can have an average diameter and the solid beads in the wash solution can have an average diameter that is at least twice the diameter of the DNA-coated beads. The washing can form dislodged DNA-coated beads and a monolayer of DNA-coated beads. In some embodiments, first beads for forming an array are contacted with a poly(ethylene glycol) (PEG) solution comprising a PEG having a molecular weight of about 350 Da or less. In some embodiments, slides for forming bead arrays are provided as are systems for imaging the same.Type: ApplicationFiled: August 31, 2010Publication date: June 9, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Mark OLDHAM, George Fry, Christina Inman, John Bridgham, Timothy Hunkapillar, Charles Vann
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Publication number: 20110124111Abstract: Various embodiments of a low-volume sequencing system are provided herein. The system can include a low-volume flowcell having at least one reaction chamber of a defined volume (e.g., less than about 100 ?l). The system can also include an automated reagent delivery mechanism configured to reversibly couple with the inlet port corresponding to a target reaction chamber thereby placing allowing for reagent to be accurately moved from a storage container to the reaction chamber with minimal reagent waste. The flowcells can include a plurality of reaction chambers (e.g., 6) thereby allowing for parallel analysis of multiple samples. Various methods of analyzing a biomolecule are also provided herein.Type: ApplicationFiled: August 31, 2010Publication date: May 26, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Jon HOSHIZAKI, Joon Mo YANG, Maryam SHARIATI, David COX, Kirk HIRANO, John BRIDGHAM, Sam WOO, George GOLDA
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Publication number: 20110052446Abstract: Various flowcell configurations and systems are provided as are methods of making and using same. The flowcells, systems, and methods of use can be useful in carrying out sequencing reactions and next generation sequencing methods.Type: ApplicationFiled: August 31, 2010Publication date: March 3, 2011Applicant: LIFE TECHNOLOGIES CORPORATIONInventors: Kirk HIRANO, Mark ANDERSEN, Jian GONG, Sam WOO, David COX, Joon Mo YANG, Min YUE, Maryam SHARIATI, John BRIDGHAM, David LIU
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Publication number: 20100111768Abstract: The present invention comprises systems and devices for sequencing of nucleic acid, such as short DNA sequences from clonally amplified single-molecule arrays.Type: ApplicationFiled: March 30, 2007Publication date: May 6, 2010Applicant: SOLEXA, INC.Inventors: Saibal Banerjee, Colin Barnes, Kevin Benson, John Bridgham, Jason Bryant, Dale Buermann, Sergey Etchin, Jonny Ho, Xavier Lee, Peter Lundberg, Klaus Maisinger, Bojan Obradovic, Mark Pratt, Isabelle Rasolonjatovo, Mark Reed, Chiara Rodighiero, Subra Sankar, Gary Schroth, Ning Sizto, Harold Swerdlow, Eric Vermaas
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Publication number: 20090143244Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.Type: ApplicationFiled: October 12, 2007Publication date: June 4, 2009Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner
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Publication number: 20090023189Abstract: Methods and systems for forming water-in-oil emulsions are described. For example, an apparatus is described which includes: a first compartment containing a plurality of particles dispersed in an aqueous phase; a second compartment containing an oil phase; a porous layer separating the first and second compartments; and a device for applying pressure to the first compartment. A method is described which includes: moving an oil phase relative to a surface of a porous layer while simultaneously forcing an aqueous composition comprising particles through the porous layer and into the flowing dispersion medium thereby forming droplets of the aqueous composition containing particles dispersed in the oil phase. The aqueous composition can include one or more nucleic acid templates and reagents for amplifying the nucleic acids such as PCR reagents.Type: ApplicationFiled: May 12, 2008Publication date: January 22, 2009Applicant: APPLERA CORPORATIONInventors: Aldrich N.K. Lau, Jonathan M. Cassel, John A. Bridgham
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Patent number: 7282370Abstract: An apparatus and system are provided for simultaneously analyzing a plurality of analytes anchored to microparticles. Microparticles each having a uniform population of a single kind of analyte attached are disposed as a substantially immobilized planar array inside of a flow chamber where steps of an analytical process are carried out by delivering a sequence of processing reagents to the microparticles by a fluidic system under microprocessor control. In response to such process steps, an optical signal is generated at the surface of each microparticle which is characteristic of the interaction between the analyte carried by the microparticle and the delivered processing reagent. The plurality of analytes are simultaneously analyzed by collecting and recording images of the optical signals generated by all the microparticles in the planar array.Type: GrantFiled: August 18, 2005Date of Patent: October 16, 2007Assignee: Solexa, Inc.Inventors: John Bridgham, Kevin Corcoran, George Golda, Michael C. Pallas, Sydney Brenner