Patents by Inventor John A. Lindbo

John A. Lindbo has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20200199605
    Abstract: The present disclosure provides compositions, methods, and systems for targeted plant genome editing. In some embodiments, the present disclosure provides recombinant self-replicating RNAs derived from a plant virus vector, such as those derived from Tobacco Mosaic Virus (TMV). In some embodiments, the recombinant self-replicating RNAs direct the expression of both a CRISPR endonuclease and a guide RNA capable of directing sequence-specific binding of the CRISPR endonuclease to a target DNA in the genome of plant cell.
    Type: Application
    Filed: June 7, 2018
    Publication date: June 25, 2020
    Inventor: John LINDBO
  • Publication number: 20150344896
    Abstract: Modified expression vectors, including Tobacco Mosaic Virus (TMV) expression vectors, methods for modifying such vectors, and uses of the same are disclosed.
    Type: Application
    Filed: December 12, 2014
    Publication date: December 3, 2015
    Applicant: THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION
    Inventor: John A. Lindbo
  • Patent number: 8936937
    Abstract: Modified expression vectors, including Tobacco Mosaic Virus (TMV) expression vectors, methods for modifying such vectors, and uses of the same are disclosed.
    Type: Grant
    Filed: January 28, 2008
    Date of Patent: January 20, 2015
    Assignee: The Ohio State University Research Foundation
    Inventor: John A. Lindbo
  • Publication number: 20110111413
    Abstract: The present invention relates to codon optimization utilizing DNA shuffling. A method of producing gene sequences optimized for a desired functional property is described involving synthesizing a library of parental codon variant genes encoding some or all codon choices at some or all amino acid positions of a gene, reassorting the variant codons among the parental codon variant genes using DNA shuffling thereby forming progeny codon variant genes, expressing the progeny codon variant genes in a host; and screening or selecting for progeny codon variant genes encoding a desired functional property.
    Type: Application
    Filed: November 23, 2010
    Publication date: May 12, 2011
    Inventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice, Andrew A. Vaewhongs
  • Patent number: 7939318
    Abstract: Herein-described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different encapsidation intermediate such that the reassembled VLP displays more than one peptide or nucleic acid. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.
    Type: Grant
    Filed: April 24, 2006
    Date of Patent: May 10, 2011
    Assignee: Kentucky Bioprocessing, LLC
    Inventors: Alison A. McCormick, Mark L. Smith, Kenneth E. Palmer, John A. Lindbo, Long V. Nguyen, Gregory P. Pogue
  • Patent number: 7838219
    Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
    Type: Grant
    Filed: August 8, 2003
    Date of Patent: November 23, 2010
    Assignee: Novici Biotech LLC
    Inventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice, Andrew A. Vaewhongs
  • Patent number: 7833759
    Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
    Type: Grant
    Filed: June 25, 2007
    Date of Patent: November 16, 2010
    Assignee: Novici Biotech LLC
    Inventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
  • Publication number: 20100071085
    Abstract: Modified expression vectors, including Tobacco Mosaic Virus (TMV) expression vectors, methods for modifying such vectors, and uses of the same are disclosed.
    Type: Application
    Filed: January 28, 2008
    Publication date: March 18, 2010
    Applicant: THE OHIO STATE UNIVERSITY RESEARCH FOUNDATION
    Inventor: John A. Lindbo
  • Patent number: 7582423
    Abstract: We describe here an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences, by making a heteroduplex polynucleotide from two non-identical polynucleotides; introducing a nick in one strand at or near a base pair mismatch site; removing mismatched base(s) from the mismatch site where the nick occurred; and using the opposite strand as template to replace the removed base(s) with bases that complement base(s) in the first strand. By this method, information is transferred from one strand to the other at sites of mismatch.
    Type: Grant
    Filed: October 25, 2002
    Date of Patent: September 1, 2009
    Assignee: Novici Biotech LLC
    Inventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
  • Publication number: 20090053261
    Abstract: Display of peptides or proteins in an ordered, repetitive array, such as on the surface of a virus-like particle, is known to induce an enhanced immune response relative to vaccination with the “free” protein antigen. The 2100 coat proteins comprising the rod-shaped capsid of Tobacco mosaic virus (TMV) can accommodate short peptide insertions into the primary sequence, but the display of larger protein moieties on the virion surface by genetic fusions to the capsid protein has not been possible. Since TMV lacks surface exposed residues compatible with commonly available linker chemistries, we employed a randomized library approach to introduce a reactive lysine at the externally located at the amino-terminus of the coat protein. We found that we could easily control the extent of virion conjugation and demonstrated stoichiometric biotinylation of the introduced lysine.
    Type: Application
    Filed: September 8, 2006
    Publication date: February 26, 2009
    Inventors: John A. Lindbo, Kenneth E. Palmer, Mark L. Smith
  • Patent number: 7413889
    Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.
    Type: Grant
    Filed: February 4, 2002
    Date of Patent: August 19, 2008
    Assignee: Kentucky Bioprocessing, LLC
    Inventors: Gregory P. Pogue, John A. Lindbo, Michael J. McCulloch, Jonathan E. Lawrence, Cynthia S. Gross, Stephen J. Garger
  • Publication number: 20080032346
    Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
    Type: Application
    Filed: June 25, 2007
    Publication date: February 7, 2008
    Applicant: LARGE SCALE BIOLOGY CORPORATION
    Inventors: Hal Padgett, John Lindbo, Wayne Fitzmaurice
  • Patent number: 7297478
    Abstract: Disclosed are methods and compositions for creating a DNA, RNA or protein molecule with two or more nucleic acid or polypeptide domains, respectively, joined by a linker region. These methods are used to generate random linker libraries of nucleic acids that encode dual-domain or multi-domain polypeptides. The linker regions are characterized by both length and sequence variability.
    Type: Grant
    Filed: September 22, 2000
    Date of Patent: November 20, 2007
    Assignee: Large Scale Biology Corporation
    Inventors: Stephen J. Reinl, John A. Lindbo, Thomas Turpen
  • Patent number: 7270825
    Abstract: The present invention relates to foreign peptide sequences fused to recombinant plant viral structural proteins and a method of their production. Fusion proteins are economically synthesized in plants at high levels by biologically contained tobamoviruses. The fusion proteins of the invention have are useful as antigens for inducing the production of antibodies having desired binding properties, e.g., protective antibodies, or for use as vaccine antigens for the induction of protective immunity against the parvovirus. Feline parvovirus epitopes were fused to the N-terminus of the TMV coat protein, expressed in Nicotiana plants, extracted, purified, characterized and administered to animals, resulting in protective immunity.
    Type: Grant
    Filed: July 12, 2002
    Date of Patent: September 18, 2007
    Assignee: Large Scale Biology Corporation
    Inventors: Gregory P. Pogue, John A. Lindbo, Michael J. McCulloch, Jonathan E. Lawrence, Cynthia S. Gross, Stephen J. Garger
  • Patent number: 7235386
    Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
    Type: Grant
    Filed: July 25, 2002
    Date of Patent: June 26, 2007
    Assignee: Large Scale Biology Corporation
    Inventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
  • Patent number: 7217514
    Abstract: We describe here an in vitro method of increasing complementarity in a heteroduplex polynucleotide sequence. The method uses annealing of opposite strands to form a polynucleotide duplex with mismatches. The heteroduplex polynucleotide is combined with an effective amount of enzymes having strand cleavage activity, 3? to 5? exonuclease activity, and polymerase activity, and allowing sufficient time for the percentage of complementarity to be increased within the heteroduplex. Not all heteroduplex polynucleotides will necessarily have all mismatches resolved to complementarity. The resulting polynucleotide is optionally ligated. Several variant polynucleotides result. At sites where either of the opposite strands has templated recoding in the other strand, the resulting percent complementarity of the heteroduplex polynucleotide sequence is increased. The parent polynucleotides need not be cleaved into fragments prior to annealing heterologous strands. Therefore, no reassembly is required.
    Type: Grant
    Filed: July 25, 2002
    Date of Patent: May 15, 2007
    Assignee: Large Scale Biology Corporation
    Inventors: Hal S. Padgett, John A. Lindbo, Wayne P. Fitzmaurice
  • Patent number: 7132588
    Abstract: The present invention provides nucleic acid sequences having an altered viral movement protein and 126/183 kDa replicase proteins further characterized in its ability tostabilize a transgene contained in a virus that expresses the altered movement protein. The present invention also provides viral vectors expressing the altered movement protein, cells transformed with the vectors, and host plants infected by the viral vectors.
    Type: Grant
    Filed: July 21, 2003
    Date of Patent: November 7, 2006
    Assignee: Large Scale Biology Corporation
    Inventors: Wayne P. Fitzmaurice, Gregory P. Pogue, John A. Lindbo
  • Publication number: 20060188991
    Abstract: Herein-described are various methods for making a vaccine that are made of re-assembled virus like particles (VLP). First, the VLPs are disassembled into encapsidation intermediate populations. Each encapsidation intermediate population undergoes, for instance, chemical conjugation of unique peptide or nucleic moieties to form separate populations. Thereafter, a predetermined amount of each of the several (one or more) different encapsidation intermediates from the different populations is mixed and joined, forming intact VLPs, surrounding a nucleic acid core, that are composed of different encapsidation intermediate such that the reassembled VLP displays more than one peptide or nucleic acid. The nucleic acid can function either as a scaffold alone or can be engineered for the expression of an immunomodulatory protein in a eukaryotic cell.
    Type: Application
    Filed: April 24, 2006
    Publication date: August 24, 2006
    Inventors: Alison McCormick, Mark Smith, Kenneth Palmer, John Lindbo, Long Nguyen, Gregory Pogue
  • Patent number: 7084256
    Abstract: A polypeptide self-antigen useful in a tumor-specific vaccine mimics one or more epitopes of an antigen uniquely expressed by cells of the tumor. The polypeptide is preferably produced in a plant that has been transformed or transfected with nucleic acid encoding the polypeptide and is obtainable from the plant in correctly folded, preferably soluble form without a need for denaturation and renaturation. This plant-produced polypeptide is immunogenic without a need for exogenous adjuvants or other immunostimulatory materials. The polypeptide is preferably an scFv molecule that bears the idiotype of the surface immunoglobulin of a non-Hodgkin's (or B cell) lymphoma. Upon administration to a subject with lymphoma, the plant-produced, tumor-unique scFv polypeptide induces an idiotype-specific antibody or cell-mediated immune response against the lymphoma.
    Type: Grant
    Filed: February 8, 2002
    Date of Patent: August 1, 2006
    Assignee: Large Scale Biology Corporation
    Inventors: Alison A. McCormick, Daniel Tusé, Stephen J. Reinl, John A. Lindbo, Thomas H. Turpen
  • Publication number: 20060134604
    Abstract: A plurality of proteins of interest, or peptides of interest, or other genetically expressed materials, are screened and subsequently produced using any of a variety of expression systems. The plurality of proteins are extracted from a plurality of separate, processed green juices, each green juice containing one of the proteins of interest. A multi-channel apparatus processes the various green juices, one green juice per channel. The apparatus is computer controlled such that the various valves in each channel and pump are controlled in an automated manner to extract each protein of interest and deliver each protein of interest into its own storage vessel.
    Type: Application
    Filed: December 16, 2005
    Publication date: June 22, 2006
    Inventors: Mark Smith, Kenneth Palmer, Gregory Pogue, John Lindbo, Kathleen Hanley, David Mannion, Gershon Wolfe