Abstract: Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.

Abstract: The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

Abstract: Provided herein are chimeric nucleic acid sequences encoding chimeric polypeptides. Also provided herein are chimeric polypeptides. Further provided herein are methods of treating a subject with or at risk of developing a cancer. The methods comprise selecting a subject with or at risk of developing a cancer, and administering to the subject an effective amount of the chimeric polypeptides provided herein.

Abstract: Provided herein are chimeric nucleic acid sequences encoding chimeric polypeptides. Also provided herein are chimeric polypeptides. Further provided herein are methods of treating a subject with or at risk of developing a cancer. The methods comprise selecting a subject with or at risk of developing a cancer, and administering to the subject an effective amount of the chimeric polypeptides provided herein.

Abstract: A hand-operated surgical instrument with sequentially engaged tip closing guides. The instrument includes first and second arms having tips that close during a closure operation. A slotted tip closure guide on the arms engages first during the closure operation. A pin closure guide on the arms between the slotted closure guide and the tips engages during the closure operation after the engagement of the slotted closure guide. The sequential engagement of the guides provides precise approximation of the instrument tips and prevents “scissoring” of the tips.

Abstract: Controlled cessation of heart beat during coronary bypass surgery and other cardiac surgeries on a beating heart improves surgical technique, and is achieved typically by electrical stimulation of the vagus nerve and administration of a combination of drugs.

Abstract: Disclosed herein are methods and kits for the detection of human immunodeficiency virus in biological samples from human subjects. Oligonucleotide reverse transcription primers for use in such methods and kits for detection of human immunodeficiency virus are also described.