Abstract: Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload inhibits expression or activity of DUX4. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide or RNAi oligonucleotide.

Abstract: A reservoir and method for manufacturing a method may be provided, the reservoir may include a first side piece, a second side piece, an inner fold piece and a reservoir fitting. The first side piece may be sealed to the second side piece with a top seal and a first side seal and a second side seal. The inner fold piece may be sealed to the first side piece and the second side piece through the first side seal, the second side seal, and a bottom seal. The top seal may include a fitting opening and the reservoir fitting may be fit into the fitting opening. The reservoir may contain a volume sensing system. The reservoir may be designed to shape and form to be optimized anatomically so as is to empty and fill reliably without any concern for neighboring organ impingement or compression.

Abstract: The present application relates to oligonucleotides (e.g., antisense oligonucleotides such as gapmers) designed to target DMPK RNAs and targeting complexes for delivering the oligonucleotides to cells (e.g., muscle cells) and uses thereof, particularly uses relating to treatment of disease. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload inhibits expression or activity of DMPK.

Abstract: One aspect of the present application relates to a pharmaceutical composition comprising a sulfated glycosaminoglycan having an average molecular weight ranging from 3,000 to 15,000 Daltons, a skin penetrating agent, where the skin penetrating agent promotes transdermal penetration of the sulfated glycosaminoglycan when applied to intact skin surfaces, and a pharmaceutical carrier for topical application, where the pharmaceutical carrier is mixed with the sulfated glycosaminoglycan and the skin penetrating agent. This composition is useful in carrying out a method of treating a subject for pain, joint stiffness, soft tissue injury, connective tissue disorders, and/or muscle soreness.

Abstract: Aspects of the disclosure relate to oligonucleotides (e.g., RNAi oligonucleotides such as siRNAs) designed to target DUX4 RNAs and targeting complexes for delivering the oligonucleotides to cells (e.g., muscle cells) and uses thereof, particularly uses relating to treatment of disease (e.g., FSHD).

Abstract: Nucleic acids encoding C1 inhibitor are described. Also described are expression cassettes, vectors, cells, and cell lines containing the nucleic acids, as well as methods of using the nucleic acids to treat complement-mediated disorders, such as hereditary angioedema.

Abstract: This disclosure describes a system, method, and non-transitory computer readable media for an ultrasound probe configured to capture ultrasound images of an examination region. The system includes a first set of one or more sensors coupled to the ultrasound probe and configured to estimate a first positional information associated with the ultrasound probe. The system includes a second set of one or more sensors coupled to the ultrasound probe and configured to capture electromagnetic force (EMF) measurements in the examination region to estimate a second positional information associated with the ultrasound probe. The second positional information is used to calibrate the first set of one or more sensors. The system includes a controller configured to use at least one of (i) the first positional information, or (ii) the second positional information to generate a reconstruction of the examination region based on ultrasound images captured by the ultrasound probe.

Abstract: Aspects of the disclosure relate to complexes and other aspects relate to formulations (e.g., aqueous, lyophilized forms) comprising such complexes comprising an oligonucleotide (e.g., useful for targeting DMPK) covalently linked to an antibody (e.g., anti-TfR1 antibody).

Abstract: A bidirectional electroosmotic pump may be provided. The bidirectional electroosmotic pump may be made of materials that are biocompatible and non-ferrous. The bidirectional electroosmotic pump may be part of an implantable medical device for the purpose of medicine delivery. The bidirectional electroosmotic pump may contain a working fluid and may facilitate the delivery of a separate payload fluid. In an exemplary embodiment, the bidirectional pump may contain bellows which may allow the pump to deliver the payload fluid through a series of valves and/or catheters. In another embodiment the bidirectional electroosmotic pump may contain a pump sensing mechanism to monitor the state of the pump.

Abstract: Aspects of the disclosure relate to complexes comprising a muscle-targeting agent covalently linked to a molecular payload. In some embodiments, the muscle-targeting agent specifically binds to an internalizing cell surface receptor on muscle cells. In some embodiments, the molecular payload inhibits expression or activity of DUX4. In some embodiments, the molecular payload is an oligonucleotide, such as an antisense oligonucleotide or RNAi oligonucleotide.

Abstract: A system that analyzes images of a shelf to determine the available shelf space. Camera images of the shelf from multiple viewpoints may be projected onto the shelf surface to remove distortions from camera projections and to align images to a common shelf reference frame. A mask may be calculated from each projected image that identifies regions that match the appearance of the shelf surface. The shelf surface may have a specific pattern to facilitate identification of these regions. A combined mask may be formed as a union of the masks from individual projected image masks. The available shelf space corresponds to the regions in the combined mask. Combining image masks from multiple viewpoints reduces the effect of occlusion of the shelf surface by items on the shelf.

Abstract: A system that determines the items on a shelf by projecting camera images to a surface aligned with the tops of the items. Projecting the images to the top surface removes distortions due to camera projections and aligns multiple images to a common reference frame. Item tops may be visible without occlusion in one or more camera images, simplifying item identification. The projected images may be input into an item detector that is trained to recognize images of the tops of items. The item detector may process projected images from different cameras with parallel feature extractor subnetworks that generate feature maps; the feature maps may then be averaged across images and the averaged feature map may be input into an item detection subnetwork.

Abstract: Aspects of the present disclosure include methods for processing cytometer data, such as for group-wise analysis of the cytometer data (e.g., flow cytometry data in FCS format, mass cytometry data, genomic cytometry data). Methods according to certain embodiments include generating a compound population of events that include data accessors from cytometry data, such as where the compound population of cytometer data is from two or more different samples retained as separate raw data files (e.g., are not concatenated to form a single combined data file). Systems having an input module for receiving cytometer data and processor with memory having instructions for practicing the subject methods are also described. Non-transitory computer readable storage medium is also provided.

Abstract: System that facilitates rapid onboarding of an autonomous (cashier-less) store by capturing images of the store's items from multiple angles, with varying background colors, and that builds a classifier training dataset from these images. Background surfaces may for example be coated with retroreflective tape or film, and variable-color incident light sources may generate the desired background colors. Embodiments may automatically rotate or otherwise reorient the item placed in the onboarding system, so that a relatively small number of cameras can capture views from multiple angles. When an item is placed in the system, a fully automated process may generate a sequence of item orientations and background colors, and may capture and process images from the cameras to create training images. Images of the item from multiple angles, under varying lighting conditions, may be captured without requiring an operator to move or reorient the item.

Abstract: A phase change material heat sink including: a carbon graphite matrix having one or more removed portions; and an expanded graphite located within the one or more removed portions.

Abstract: A monolithic open cell structure apparatus including an open cell structure housing including a first side, a second side opposite the first side, a first opening located at the first side, and a second opening located opposite the first opening at a second side. The monolithic open cell structure apparatus also including a filtration portion extending from the first side to the second side within the open cell structure housing. The monolithic open cell structure apparatus further including a retaining partition that at least partially encloses the filtration portion within the open cell structure housing. The monolithic open cell structure apparatus is a single piece including a unitary structure.

Abstract: A control system in an atmospheric suit includes a broadcast-type controller area network (CAN) bus and a plurality of motor controllers coupled to the CAN bus. Each of the plurality of motor controllers has a same software design and performs a different control operation based on an assigned hardware address. The control system also includes one or more sensors to sense one or more parameter values in the atmospheric suit and to provide the one or more parameter values on the CAN bus. A primary controller communicates with the plurality of motor controllers via the CAN bus and provides communication to a wearer of the atmospheric suit or communication outside the atmospheric suit outside the CAN bus.

Abstract: Cleaning compositions having engineered fatty acid alpha-dioxygenases and methods of using said compositions to provide a benefit by converting long chain fatty acids present in soils into 2-hydroperoxy fatty acids or terminal aldehydes.

Abstract: An extravehicular mobility unit (EMU) includes a resonant coil on a surface of the EMU to be coupled to a second resonant coil affixed to a structure via a resonant magnetic field. The EMU also includes a receiver in the EMU coupled to the resonant coil to provide a direct current (DC) voltage based on the resonant magnetic field. A battery in the EMU is charged based on the DC voltage.