Abstract: Use of one or more markers to identify and sort cells that have been engineered to comprise a synthetic chromosome encoding the marker(s), with said cells expressing said marker(s) encoded on the synthetic chromosome. The marker is typically but not limited to a cluster of differentiation (CD), wherein the CD has been truncated or otherwise modified so that the biological function of the CD is lost or remarkably reduced. The modified CD is typically used to sort cells to obtain a desired population of cells containing the synthetic chromosome, or i) to ensure that a cell population administered to a subject, e.g., suffering from cancer, reaches the target tissue, ii) to ensure that a cell population administered to a subject, e.g.

Abstract: The risk with introducing manipulated T-cell is unforeseen adverse events. During the development of chimeric antigen receptor (CAR) T-cell therapies almost all clinical trial has shown some adverse events ranging from cytokine mediated toxicities to tissue damage and death. By the present invention, we aim to induce multiple layers of safety checkpoints. As a last resort we will be able to induce suicide in all cells which we have introduced to the body. Here we describe the scientific background to the parts of our suicide switch and the details regarding the proteins which are included. Safety switches are being tested on CAR-T cells, but they have a few drawbacks. Due to the limited space on the CAR vector, there is only room for one gene switch. Presently the results show they induce apoptosis in around 70-90% of cells, while the desired target is for all cells to be removed from the tissue.

Abstract: The present document discloses a tool or tool part for use in a process of molding a product from a pulp slurry. The tool or tool part comprises a self-supporting tool wall portion having a product face, for contacting the product, and a back face on the other side of the wall relative to the product face. The tool wall portion presenting pores, which are provided by a plurality of channels extending through the tool wall portion, from the product face to the back face. The channels are straight or curved with no more than one point of inflection.

Abstract: The present document discloses a tool or tool part for use in a process of molding a product from a pulp slurry. The tool or tool part comprises a self-supporting tool wall portion having a product face, for contacting the product, and a back face on the other side of the wall relative to the product face. The tool wall portion presenting pores, which are provided by a plurality of channels extending through the tool wall portion, from the product face to the back face. The channels are straight or curved with no more than one point of inflection.

Abstract: Aspects of the present invention relate to the use of chemokine receptors such as e.g., CCR4 and CCR6, as homing agents. The chemokine receptor(s) is/are expressed by cells containing synthetic chromosomes. The chemokine receptor(s) is/are under controllable expression from a synthetic chromosome and can be used to control an immune response so as to treat or inhibit a disease such as cancer. The control is provided by inducing leukocyte trafficking to sites of disease. If more than one chemokine receptor is expressed, the levels can be individually controlled so that the desired cellular trafficking pattern can be obtained.

Abstract: Aspects of the present invention relate to synthetic chromosomes that may be incorporated into leukocytes, wherein the synthetic chromosomes comprise nucleic acid sequences encoding multiple Chimeric Antigen Receptors (CARs). Such manipulated leukocytes can be used in medicine, notably in the treatment of a cancer such as in the treatment of cancer having solid tumors. The leukocytes may be lymphocytes, including tumor-infiltrating lymphocytes. T cells. NK cells or B cells. In a preferred aspect, the leucocytes are syngeneic and T cells.

Abstract: Aspects of the present invention relate to the use the growth factors such as e.g., cytokines IL-2 and IL-12 for T-cell activation whereby the growth factor(s) is/are expressed by cells comprising synthetic chromosomes. The growth factor(s) is/are desirably under controllable expression from a synthetic chromosome. By approaches described herein, one can control an immune response to treat or inhibit a disease such as a cancer. The control may be provided by inducing expression of the growth factor(s), wherein expression level(s) can be fine-tuned. If more than one growth factor is expressed, the levels can be individually controlled so that the desired concentrations of each growth factor are obtained.

Abstract: The present document discloses a tool or tool part for use in a process of molding a product from a pulp slurry. The tool or tool part comprises a self-supporting tool wall portion having a product face, for contacting the product, and a back face on the other side of the wall relative to the product face. The tool wall portion presenting pores, which are provided by a plurality of channels extending through the tool wall portion, from the product face to the back face. The channels are straight or curved with no more than one point of inflection.

Abstract: The present document discloses a tool or tool part for use in a process of molding a product from a pulp slurry. The tool or tool part comprises a self-supporting tool wall portion having a product face, for contacting the product, and a back face on the other side of the wall relative to the product face. The tool wall portion presenting pores, which are provided by a plurality of channels extending through the tool wall portion, from the product face to the back face. The channels are straight or curved with no more than one point of inflection.

Abstract: The present document discloses a tool or tool part for use in a process of molding a product from a pulp slurry. The tool or tool part comprises a self-supporting tool wall portion having a product face, for contacting the product, and a back face on the other side of the wall relative to the product face. The tool wall portion presenting pores, which are provided by a plurality of channels extending through the tool wall portion, from the product face to the back face. The channels are straight or curved with no more than one point of inflection.

Abstract: The present document discloses a tool or tool part for use in a process of molding a product from a pulp slurry. The tool or tool part comprises a self-supporting tool wall portion having a product face, for contacting the product, and a back face on the other side of the wall relative to the product face. The tool wall portion presenting pores, which are provided by a plurality of channels extending through the tool wall portion, from the product face to the back face. The channels are straight or curved with no more than one point of inflection.

Abstract: The present document discloses a tool or tool part for use in a process of molding a product from a pulp slurry. The tool or tool part comprises a self-supporting tool wall portion having a product face, for contacting the product, and a back face on the other side of the wall relative to the product face. The tool wall portion presenting pores, which are provided by a plurality of channels extending through the tool wall portion, from the product face to the back face. The channels are straight or curved with no more than one point of inflection.

Abstract: The present document discloses a tool or tool part for use in a process of molding a product from a pulp slurry. The tool or tool part comprises a self-supporting tool wall portion having a product face, for contacting the product, and a back face on the other side of the wall relative to the product face. The tool wall portion presenting pores, which are provided by a plurality of channels extending through the tool wall portion, from the product face to the back face. The channels are straight or curved with no more than one point of inflection.

Abstract: The present document discloses a tool or tool part for use in a process of molding a product from a pulp slurry. The tool or tool part comprises a self-supporting tool wall portion having a product face, for contacting the product, and a back face on the other side of the wall relative to the product face. The tool wall portion presenting pores, which are provided by a plurality of channels extending through the tool wall portion, from the product face to the back face. The channels are straight or curved with no more than one point of inflection.

Abstract: Methods and computer apparatus are disclosed for deriving first order logic language rules for use in analysing new data to identify anomalies in the new data. Historical data is formatted to be in a form suitable for application of a machine-learning algorithm thereto. The machine-learning algorithm is applied to the formatted historical data to generate a set of first order logic language rules that cover the formatted historical data. The set of first order logic language rules are analysed to obtain a subset of said set of first order logic language rules which can be used as first order logic language rules in analysing new data.

Abstract: An apparatus for supporting a sheathing material comprises a mandrel containing magnetically conductive material, and a plurality of spaced magnets. The magnets contain aligned circular apertures. The mandrel is centered within the apertures by a magnetic field applied substantially uniformly around the periphery of the mandrel. The mandrel is thus supported in a freely suspended manner. Sheathing material can be inserted onto the freely suspended mandrel and advanced freely therealong so as to be treated while mounted on the mandrel.