Patents by Inventor John B. McARTHUR

John B. McARTHUR has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • METHODS FOR PRODUCTION OF FUCOSYLATED OLIGOSACCHARIDES IN RECOMBINANT CELL CULTURE
    Publication number: 20240011064
    Abstract: Methods for producing oligosaccharide products such as difucosylated oligosaccharides are disclosed. The methods include culturing recombinant cells in a cell culture medium comprising L-fucose, an oligosaccharide acceptor, and a carbon source. The cells are cultured under conditions in which a first fucosyltransferase polypeptide having a first substrate selectivity (e.g., an ?1-2-fucosyltransferase polypeptide), a second fucosyltransferase polypeptide having a second substrate specificity (e.g., an ?1-3-fucosyltransferase polypeptide), a nucleotide sugar pyrophosphorylase polypeptide, a lactose transporter polypeptide, and an L-fucose transporter polypeptide are expressed, and in which the oligosaccharide acceptor is converted to the difucosylated oligosaccharide. Recombinant cells for use in the methods are also described.
    Type: Application
    Filed: August 15, 2023
    Publication date: January 11, 2024
    Applicant: The Regents of the University of California
    Inventors: Shota ATSUMI, Angela ZHANG, Xi CHEN, Yuanyuan BAI, Hai YU, John B. McARTHUR
  • Sialyltransferase variants having neosialidase activity
    Patent number: 11739305
    Abstract: ?2-6-Sialyltransferase (2,6ST) variants having improved ?2-6-specific sialidase activity as compared to the native 2,6ST enzymes are described. The variants include GT80 sialyltransferases such as P. damselae Pd2,6ST. Methods for making de-sialylated products and screening sialidase activity are also described.
    Type: Grant
    Filed: December 21, 2018
    Date of Patent: August 29, 2023
    Assignee: The Regents of the University of California
    Inventors: Xi Chen, John B. McArthur, Andrew J. Fisher
  • Heparosan synthases and use thereof for saccharide synthesis
    Patent number: 11441131
    Abstract: Heparosan synthase variants having improved expression levels, enhanced thermal stability, and/or reduced reverse glycosylation activity are provided. Methods for making oligosaccharides and polysaccharides, including heparin analogs and heparan sulfate analogs, are also described.
    Type: Grant
    Filed: June 19, 2020
    Date of Patent: September 13, 2022
    Assignee: The Regents of the University of California
    Inventors: Xi Chen, Lan Na, Hai Yu, John B. McArthur
  • HEPAROSAN SYNTHASES AND USE THEREOF FOR SACCHARIDE SYNTHESIS
    Publication number: 20210040458
    Abstract: Heparosan synthase variants having improved expression levels, enhanced thermal stability, and/or reduced reverse glycosylation activity are provided. Methods for making oligosaccharides and polysaccharides, including heparin analogs and heparan sulfate analogs, are also described.
    Type: Application
    Filed: June 19, 2020
    Publication date: February 11, 2021
    Inventors: Xi CHEN, Lan NA, Hai YU, John B. McARTHUR
  • SIALYL TRANSFERASE VARIANTS HAVING NEOSIALIDASE ACTIVITY
    Publication number: 20200325457
    Abstract: ?2-6-Sialyltransferase (2,6ST) variants having improved ?2-6-specific sialidase activity as compared to the native 2,6ST enzymes are described. The variants include GT80 sialyltransferases such as P. damselae Pd2,6ST. Methods for making de-sialylated products and screening sialidase activity are also described.
    Type: Application
    Filed: December 21, 2018
    Publication date: October 15, 2020
    Inventors: Xi CHEN, John B. McARTHUR, Andrew J. FISHER