Abstract: Provided are methods and apparatus for determining conditions for performing chromatography, particularly flash chromatography. The methods may be utilized for separating compounds in a sample in a column chromatography system. The method includes determining at least two retention factors corresponding to the TLC performance of a target compound and an adjacent compound and defining a sample loading amount and a solvent gradient profile using at least two solvents that will result in differential elution of the target and adjacent compounds from a chromatography column. The chromatography system and loading parameters may be selected to provide satisfactory separation of the target compound from adjacent compounds.

Abstract: Systems, methods, and computer program products for determining conditions for performing chromatography. A method may be provided for separating compounds in a sample by a column chromatography system. That method includes receiving input including an indication of a retention factor that includes an indication of a first retention factor of a first component of a sample compound, and an indication of a solvent ratio; generating a gradient profile based on the received input; and separating a sample compound based on the gradient profile. In that method, the solvent ratio corresponds to the first retention factor indicated by the input and is a ratio of a first solvent and a second solvent, and the gradient profile includes a gradient portion including a starting point and an ending point corresponding to a starting ratio and an ending ratio of the first and second solvents.