Abstract: A gas turbine engine including a wall assembly, a seal assembly, and a member extended through the wall assembly is provided. The wall assembly defines an opening through which the member is extended, and the wall assembly defines a first side and a second side opposite of the first side along a direction of extension of the member through the wall assembly. The seal assembly includes a retaining portion extended at least partially co-directional to the member. The retaining portion is configured to couple around the member and extend through the opening. The seal assembly further includes a locking portion configured to sealingly attach to the wall assembly and the retaining portion at an interface between the locking portion and the retaining portion.

Abstract: A cryopreservation composition generally includes a cryoprotective agent and a laser absorber that includes a metal. Rewarming a cryopreserved specimen that includes the cryopreservation composition includes subjecting the cryopreserved specimen to a laser pulse effective to heat the laser absorber sufficiently to thaw the cryopreserved biospecimen.

Abstract: Methods for cryopreservation of biological samples are provided. The biological samples are sub-millimeter or millimeter scale biological materials. The biological samples are embryos, such as Drosophila embryos. Methods for cryopreservation of Drosophila embryos using cryomesh are provided. The Drosophila embryos are collected, staged and treated to optimize the cryopreservation outcomes upon rewarming. Methods disclosed are efficient for maintaining stocks of Drosophila wild type and mutant strains. Methods are also disclosed for cryopreservation of other terrestrial organism embryos and/or aquatic organism embryos.

Abstract: A surgical kit for providing a cryoprotective composition configured to be applied during cryotreatment of a patient includes: a first container containing a predetermined amount or volume of a biodegradable and/or bioerodible fluid agent; and a second container containing a predetermined amount or volume of a non-toxic cryoprotectant agent. The predetermined amount or volume of the of biodegradable and/or bioerodible fluid agent and the predetermined amount or volume of a non-toxic cryoprotectant agent are configured to be mixed together to form the cryoprotective composition in which a therapeutically effective amount of the cryoprotective composition deposited in a body space of the patient in proximity to the cryotreatment remains within at least a portion of the body space for a duration of the cryotreatment and at least a portion of a body tissue proximate to the body space is viable after the cryotreatment.

Abstract: Methods and vitrification systems for biological samples are provided. The vitrification system has a rotatable cryowheel (210) with a facing surface (220). Droplets of a composition that includes a biological sample are released onto the facing surface. Droplets are rapidly pulled beneath the surface of the cryogenic coolant (160) to generate vitrified samples (180). Methods and cryopreservation devices are also provided that incorporate the vitrification systems.

Abstract: A cryopreservation composition generally includes a cryoprotective agent and a laser absorber that includes a metal. Rewarming a cryopreserved specimen that includes the cryopreservation composition includes subjecting the cryopreserved specimen to a laser pulse effective to heat the laser absorber sufficiently to thaw the cryopreserved biospecimen.

Abstract: A cryoprotective composition configured to be applied during cryotreatment of a patient includes at least one biodegradable and/or bioerodible fluid agent and at least one non-toxic cryoprotectant agent. A therapeutically effective amount of the cryoprotective composition deposited in a body space of the patient in proximity to the cryotreatment remains within at least a portion of the body space for a duration of the cryotreatment. At least a portion of a body tissue proximate to the body space is viable after the cryotreatment. A method of protecting a surgical site during prostate cryosurgery is also provided. The method includes injecting a therapeutically effective amount of the cryoprotective composition into the body space, wherein the body space is a periprostatic space of a patient.

Abstract: A cryopreservation system for biological samples is provided. Tire cryopreservation system includes a cooling platform 100 with a 3D printing device that enables a “pick and print” method for processing biological samples 140 for cryopreservation. A syringe or syringes 110 in the 3D printing device picks up biological samples and prints them into a cryogenic environment. A sorting station 200 sorts vitrified samples from unvitrified samples. A warming platform 300 warms the samples using a laser warming system. The cryopreservation system with the sorting station and warming platform are configured for high throughput. Methods for cooling, sorting and warming the biological samples in a high throughput manner are also provided.

Abstract: Compositions and methods for cryoprotecting a biological tissue can involve a cryoprotective agent and a metal component that includes a metal foam, a metal foil, or a metal seed. The method allows the biological tissue to be rewarmed at a rate of at least 100° C./min.

Abstract: A gas turbine engine including a wall assembly, a seal assembly, and a member extended through the wall assembly is provided. The wall assembly defines an opening through which the member is extended, and the wall assembly defines a first side and a second side opposite of the first side along a direction of extension of the member through the wall assembly. The seal assembly includes a retaining portion extended at least partially co-directional to the member. The retaining portion is configured to couple around the member and extend through the opening. The seal assembly further includes a locking portion configured to sealingly attach to the wall assembly and the retaining portion at an interface between the locking portion and the retaining portion.

Abstract: Cryoprotective compositions with magnetic nanowires and cryoprotective agents are used to cryopreserve biomaterials. The biomaterials in the cryoprotective composition are rapidly and uniformly heated during warming to minimize damage due to cracking and/or devitrification. The nanowires are CoFe nanowires. The nanowires may be aligned parallel to the alternating magnetic field to increase the specific absorption rate of the nanowires. The methods include warming the cryopreserved biomaterial in the cryopreserved composition at a rate higher than the critical warming rate using an alternating magnetic field.

Abstract: A cryopreservation composition generally includes a cryoprotective agent and a laser absorber that includes a metal. Rewarming a cryopreserved specimen that includes the cryopreservation composition includes subjecting the cryopreserved specimen to a laser pulse effective to heat the laser absorber sufficiently to thaw the cryopreserved biospecimen.

Abstract: A eutectic changing composition, including a system and method of its use. The eutectic changing composition can be used in a localized area of a biological material, such as in a mammal, where the eutectic changing composition includes as an active ingredient at least one solute effective to change a tissue eutectic freezing point at the localized area of biological material. The solute can be effective to increase the tissue eutectic freezing point of the biological material.

Abstract: Methods, compositions, and systems useful to enhance a thermal surgical procedure are described. Compositions include at least one compound effective to induce an inflammatory response in biological material identified to undergo a thermal surgical procedure. Methods and systems include providing compositions of the invention to biological materials and treating biological materials with an inflammation inducing composition for a time, amount, and type effective to induce inflammation in at least a portion of the biological material.

Abstract: Methods, compositions, and systems useful to enhance a thermal surgical procedure are described. Compositions include at least one compound effective to induce an inflammatory response in biological material identified to undergo a thermal surgical procedure. Methods and systems include providing compositions of the invention to biological materials and treating biological materials with an inflammation inducing composition for a time, amount, and type effective to induce inflammation in at least a portion of the biological material.

Abstract: Methods, compositions, and systems useful to enhance a thermal surgical procedure are described. Compositions include at least one compound effective to induce an inflammatory response in biological material identified to undergo a thermal surgical procedure. Methods and systems include providing compositions of the invention to biological materials and treating biological materials with an inflammation inducing composition for a time, amount, and type effective to induce inflammation in at least a portion of the biological material.