Patents by Inventor John Bohlen
John Bohlen has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230295665Abstract: Provided herein, inter alia, are compositions, methods, and systems for efficient genetic manipulation of myelod cells without the use of viral vectors. Further provided are strategies for gene disruption in primary myeloid cells (e.g. of human and murine origin) using electroporation-based delivery of Cas/ribonuclear proteins (RNPs). Methods provided herein including embodiments thereof can provide near population-level genetic knockout of single and multiple targets in a range of cell types without selection or enrichment. Cellular fitness and response to immunological stimuli may be unaffected by the gene editing process.Type: ApplicationFiled: August 26, 2022Publication date: September 21, 2023Inventors: Aditya Krishna MURTHY, Jaehak OH, Christopher John BOHLEN, Emily Crane FREUND, Benjamin Joseph HALEY, Jaclyn LOCK
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Publication number: 20220378259Abstract: A vacuum cleaner including a dirty air inlet, a fluid flow path extending from the dirty air inlet to a clean air outlet, a fluid flow motor positioned in the fluid flow path, and a filter assembly positioned in the fluid flow path. The filter assembly including a first cylindrical filter and a second cylindrical filter nested within and removable from the first cylindrical filter. An inner diameter of the first cylindrical filter is smaller than an outer diameter of the second cylindrical filter such that the second cylindrical filter is compressed in order to be nested within the first cylindrical filter.Type: ApplicationFiled: August 5, 2022Publication date: December 1, 2022Inventors: Kirti Kant Paulla, John Bohlen, Richard Friend, Joseph Saunders, Sergey V. Makarov
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Patent number: 11432690Abstract: A handheld vacuum cleaner including a fluid flow path, a main body including a handle, a fluid flow motor positioned in the fluid flow path, a dirt collection region, and a cyclone chamber in the fluid flow path. The cyclone chamber includes a first end wall, a second end wall, a sidewall extending along a cyclone axis, a cyclone dirt outlet formed in the sidewall, a cyclone dirty fluid inlet, and a cyclone clean fluid outlet. A duct extends between the cyclone dirt outlet and the dirt collection region. The duct includes an upstream wall and a downstream flow-diverting wall forming a downstream boundary of the cyclone dirt outlet. The duct widens between the upstream wall and the downstream flow-diverting wall in a downstream direction away from the cyclone dirt outlet.Type: GrantFiled: February 11, 2020Date of Patent: September 6, 2022Assignee: Techtronic Industries Co. Ltd.Inventors: Kirti Kant Paulla, John Bohlen, Richard Friend, Joseph Saunders, Sergey V. Makarov
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Patent number: 11357370Abstract: A handheld vacuum cleaner including a fluid flow path, a main body, a fluid flow motor, a battery, and a cyclone chamber. The fluid flow path extends from a dirty air inlet to a clean air outlet, and the main body includes a handle. The fluid flow motor is positioned in the fluid flow path. The battery is positioned below the fluid flow motor. The cyclone chamber is in the fluid flow path transverse to the dirty air inlet. The cyclone chamber includes a first end wall and a second end wall, a cyclone chamber axis passing through the first end wall and the second end wall, a cyclone dirty fluid inlet, and a cyclone clean fluid outlet. The first end wall and the second end wall of the cyclone chamber both intersect a common horizontal plane when the handheld vacuum cleaner is positioned on a horizontal surface.Type: GrantFiled: October 11, 2019Date of Patent: June 14, 2022Assignee: Techtronic Industries Co. Ltd.Inventors: John Bohlen, Richard Friend, Joseph Saunders, Sergey V. Makarov, Kirti Kant Paulla
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Patent number: 10786126Abstract: A handheld vacuum cleaner includes a fluid flow path extending from a dirty air inlet to a clean air outlet, a main body including a handle, and a fluid flow motor positioned in the fluid flow path. The vacuum cleaner further includes a dirt collection region with an openable bottom and a cyclonic separator in the fluid flow-path. The cyclonic separator includes a cyclone chamber having a first end wall and a second end wall, a cyclone dirty fluid inlet, and a cyclone clean fluid outlet. The vacuum cleaner further includes a filter chamber in the fluid flow path downstream from the cyclonic separator and upstream from the fluid flow motor. The filter chamber includes an outlet fluidiy communicating the filter chamber and the fluid flow motor and a tangential inlet fluidiy communicating the cyclonic separator and the filter chamber.Type: GrantFiled: November 10, 2016Date of Patent: September 29, 2020Assignee: Techtronic Industries Co. Ltd.Inventors: Kirti Kant Paulla, John Bohlen, Richard Friend, Joseph Saunders, Sergey V. Makarov
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Patent number: 10736475Abstract: A handheld vacuum cleaner that includes a fluid flow motor is positioned in a main body along a fluid flow path and defines a motor axis. A premotor filter is disposed in the main body and includes an annular filter media forming a filter axis. The annular filter media includes a first end, a second end, and a longitudinal filter surface extending between the first end and second end. The annular filter media surrounds a filter air passageway along the filter axis and upstream of the fluid flow motor. A debris separator is upstream of the premotor filter in the fluid flow path and defines a debris separator axis. The filter axis is generally parallel to the motor axis.Type: GrantFiled: October 11, 2019Date of Patent: August 11, 2020Assignee: Techtronic Industries Co. Ltd.Inventors: Kirti Kant Paulla, John Bohlen, Richard Friend, Joseph Saunders, Sergey V. Makarov
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Publication number: 20200221910Abstract: A handheld vacuum cleaner including a fluid flow path, a main body including a handle, a fluid flow motor positioned in the fluid flow path, a dirt collection region, and a cyclone chamber in the fluid flow path. The cyclone chamber includes a first end wall, a second end wall, a sidewall extending along a cyclone axis, a cyclone dirt outlet formed in the sidewall, a cyclone dirty fluid inlet, and a cyclone clean fluid outlet. A duct extends between the cyclone dirt outlet and the dirt collection region. The duct includes an upstream wall and a downstream flow-diverting wall forming a downstream boundary of the cyclone dirt outlet. The duct widens between the upstream wall and the downstream flow-diverting wall in a downstream direction away from the cyclone dirt outlet.Type: ApplicationFiled: February 11, 2020Publication date: July 16, 2020Inventors: Kirti Kant Paulla, John Bohlen, Richard Friend, Joseph Saunders, Sergey V. Makarov
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Publication number: 20200037836Abstract: A handheld vacuum cleaner including a dirty air inlet, a fluid flow path, a main body, a fluid flow motor, a premotor filter, and a debris separator. The dirty air inlet is disposed at the front of the handheld vacuum cleaner and defines an inlet axis. The fluid flow path extends from the dirty air inlet to a clean air outlet. The main body includes a handle disposed at the rear of the handheld vacuum cleaner. The fluid flow motor is positioned in the main body along the fluid flow path and defines a motor axis. The premotor filter is disposed in the main body and includes an annular filter media forming a filter axis. The annular filter media includes a first end, a second end, and a longitudinal filter surface extending between the first end and second end. The annular filter media surrounds a filter air passageway along the filter axis and upstream of the fluid flow motor. The debris separator is upstream of the premotor filter in the fluid flow path and defines a debris separator axis.Type: ApplicationFiled: October 11, 2019Publication date: February 6, 2020Inventors: Kirti Kant Paulla, John Bohlen, Richard Friend, Joseph Saunders, Sergey V. Makarov
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Publication number: 20200037835Abstract: A handheld vacuum cleaner including a fluid flow path, a main body, a fluid flow motor, a battery, and a cyclone chamber. The fluid flow path extends from a dirty air inlet to a clean air outlet, and the main body includes a handle. The fluid flow motor is positioned in the fluid flow path. The battery is positioned below the fluid flow motor. The cyclone chamber is in the fluid flow path transverse to the dirty air inlet. The cyclone chamber includes a first end wall and a second end wall, a cyclone chamber axis passing through the first end wall and the second end wall, a cyclone dirty fluid inlet, and a cyclone clean fluid outlet. The first end wall and the second end wall of the cyclone chamber both intersect a common horizontal plane when the handheld vacuum cleaner is positioned on a horizontal surface.Type: ApplicationFiled: October 11, 2019Publication date: February 6, 2020Inventors: John Bohlen, Richard Friend, Joseph Saunders, Sergey V. Makarov, Kirti Kant Paulla
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Publication number: 20180333021Abstract: A handheld vacuum cleaner includes a fluid flow path extending from a dirty air inlet to a clean air outlet, a main body including a handle, and a fluid flow motor positioned in the fluid flow path. The vacuum cleaner further includes a dirt collection region with an openable bottom and a cyclonic separator in the fluid flow-path. The cyclonic separator includes a cyclone chamber having a first end wall and a second end wall, a cyclone dirty fluid inlet, and a cyclone clean fluid outlet. The vacuum cleaner further includes a filter chamber in the fluid flow path downstream from the cyclonic separator and upstream from the fluid flow motor. The filter chamber includes an outlet fluidiy communicating the filter chamber and the fluid flow motor and a tangential inlet fluidiy communicating the cyclonic separator and the filter chamber.Type: ApplicationFiled: November 10, 2016Publication date: November 22, 2018Applicant: Techtronic Industries Co., Ltd.Inventors: John Bohlen, Richard Friend, Joseph Saunders, Sergey V. Makarov, Kirti Kant Paulla
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Publication number: 20150054619Abstract: A distress signaling system capable of withstanding natural disasters, manmade disasters, and other similar events comprising audible and visual signaling devices; a radio frequency receiver/transmitter system; powered by a rechargeable power source coupled with solar cells; and optionally a GPS emergency beacon, a two way communicator, a camera, and an cellular phone application activator; and an installation form enabling the consumer to quickly and easily install the system with no specialized tools or knowledge.Type: ApplicationFiled: August 21, 2014Publication date: February 26, 2015Applicant: HITBox Alarm Co., L.L.C.Inventors: John Bohlen, Richard Evans, JR., Jerry Randall
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Publication number: 20080000205Abstract: An air cleaner including an improved airflow path is provided according to the invention. The air cleaner includes a duct extending between an air inlet of the air cleaner and an air outlet, with the duct being configured to conduct an airflow. The duct includes a shaped input region located downstream of one or more air cleaning components and upstream of an impeller, a curved transition region downstream of the shaped input region, with the curved transition region transitioning the airflow from an air inlet orientation to an air outlet orientation, and an expansion output region downstream of the curved transition region, with the expansion output region allowing the airflow to expand. The air cleaner further includes the impeller located in the curved transition region of the air duct. The airflow is smoothly conducted from the air inlet to the air outlet by the duct.Type: ApplicationFiled: June 30, 2006Publication date: January 3, 2008Inventor: John Bohlen
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Publication number: 20070183941Abstract: An air cleaner for ozone and Volatile Organic Compound (VOC) removal is provided according to an embodiment of the invention. The air cleaner includes an air channel in the air cleaner, an air moving unit located in the air channel and configured to create an airflow, and a combination ozone/VOC removing element located in the air channel. The combination ozone/VOC removing element substantially removes ozone and VOCs in the airflow.Type: ApplicationFiled: February 7, 2006Publication date: August 9, 2007Inventor: John Bohlen
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Publication number: 20060277876Abstract: A carbon filter panel for an air cleaner is provided according to an embodiment of the invention. The carbon filter panel includes a frame including one or more side members and a frame interior space and a plurality of spaced-apart carbon elements extending across the frame interior space. Airflow can pass between the plurality of carbon elements and through the carbon filter panel.Type: ApplicationFiled: June 8, 2005Publication date: December 14, 2006Applicant: Oreck Holdings, LLCInventor: John Bohlen
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Publication number: 20060130657Abstract: A tower ionizer air cleaner is provided. The tower ionizer air cleaner includes a tower chassis, with a base of the tower chassis including a small footprint, one or more airflow inlet openings in the tower chassis, one or more airflow outlet openings in the tower chassis and substantially opposite to the one or more airflow inlet openings, and an ionizer element positioned within the tower chassis. The tower ionizer air cleaner further includes one or more fan units substantially vertically located within the tower ionizer air cleaner and affixed to the tower chassis. The one or more fan units are configured to provide an airflow between the one or more airflow inlet openings and the one or more airflow outlet openings and through the ionizer element.Type: ApplicationFiled: December 22, 2004Publication date: June 22, 2006Applicant: Oreck Holdings, LLCInventors: John Bohlen, Thomas Oreck, Bruce Kiern, Christopher Paterson, Charles Reynolds
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Publication number: 20050124191Abstract: An apparatus for protecting an accessory connector (12) on a communication device (10). The communication device can include an opening (14) for providing access to the connector. The apparatus can be a cover (16) attached in a tethered relation to the communication device. A portion of the cover can be flexible so as to allow a user to selectively move the cover between a first position and a second position. In the first position, at the cover can substantially close the opening such that the connector is substantially protected from the infiltration of solid particles and the gross intrusion of liquids. In the second position, the cover can be held away from the connector such that substantially unimpeded access to the connector is permitted. The cover and communication device can have various associated features to ensure that the cover is retained in the first and second positions.Type: ApplicationFiled: December 4, 2003Publication date: June 9, 2005Inventors: Stephen Stanton, James Isbell, Matthew Michieli, John Bohlen, Aaron Allen, David Blatt, Sam Hosseini, Dave Karl, Eduardo Nogueiras, Peter Iezzi
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Patent number: 6118197Abstract: A refrigerator condenser fan motor enclosure including a base and a cover is described. The base and cover forming a top and a bottom of the enclosure which protects the refrigerator condenser fan motor from exposure to air borne contaminants. The base includes a first leveled step and a second leveled step which are mechanically connected to stator laminations of the condenser fan motor, a ground terminal for the condenser fan motor, and mounting lugs which allow the enclosure to be mounted onto a refrigerator. The cover is attached by tabs which are inserted into slots in the base.Type: GrantFiled: February 26, 1999Date of Patent: September 12, 2000Assignee: General Electric CompanyInventor: John Bohlen