Abstract: This disclosure relates to catalytic nucleic acids, catalytic nucleic acid probes, biosensors, lateral flow devices, and kits for detecting the presence or absence of eosinophil peroxidase. Also provided are methods for detecting the presence or absence of eosinophil peroxidase in a test sample, using the catalytic nucleic acids, catalytic nucleic acid probes, biosensors, lateral flow devices, and kits.

Abstract: Multivalent trident aptamers comprising the general formula [A-SA]2 or 3-L-[SB-B] in which a central branched linker molecule (L) possesses 2 or 3 variable arms ([A-SA]2 or 3), and a root ([SB-B]), connected by a central carbon atom, to provide enhanced affinity and/or avidity with a target are described, as well as methods of making and using the multivalent trident aptamers are provided.

Abstract: A door latch system comprises an interchangeable latch assembly for securing a door to a door frame. The interchangeable latch assembly comprises a head and a latch mounted to the head. The latch is moveable between latched and unlatched positions. A driven member is operatively connected to the latch, the driven member moveable to latch or unlatch the latch. The door latch system further comprises a universal actuating assembly removably couple-able to the interchangeable latch assembly. The universal actuating assembly comprises a mounting bracket and a driving member releasably coupled to the driven member. Actuation of the universal actuating assembly translates the driving member and the driven member, moving the latch to the unlatched position. A first interchangeable latch assembly may be swapped with a second interchangeable latch assembly without requiring modification of the universal actuating assembly.

Abstract: This disclosure relates to a method of identifying or producing an aptamer capable of binding to at least two target analyte variants, the method comprises generating a mixture of systematic evolution of ligands by exponential enrichment (SELEX)-selected aptamers by at least one-round of SELEX with a SELEX-compatible aptamer pool against the at least two target analyte variants in parallel or sequentially, sequencing the mixture of SELEX-selected aptamers that form complexes with each of the at least two target analyte variants by high-throughput sequencing, aligning the mixture of SELEX-selected aptamers that form complexes with each of the at least two target analyte variants by sequence alignment analysis, and identifying or producing the aptamer capable of binding to the at least two target analyte variants. Aptamers thereof and uses of the aptamers thereof are also disclosed.

Abstract: This disclosure relates to recognition moieties, biosensors, biosensor systems and kits thereof, and the methods for their use in detecting a target nucleic acid molecule in a test sample, including viral RNA and methods for determining whether a subject has a viral infection. The methods disclosed herein include detecting a viral infection in a subject comprising testing a sample from the subject for the presence of a target nucleic acid using a biosensor system, wherein presence of a target nucleic acid indicates that the subject has a viral infection.

Abstract: A door latch system comprises an interchangeable latch assembly for securing a door to a door frame. The interchangeable latch assembly comprises a head and a latch mounted to the head. The latch is moveable between latched and unlatched positions. A driven member is operatively connected to the latch, the driven member moveable to latch or unlatch the latch. The door latch system further comprises a universal actuating assembly removably couple-able to the interchangeable latch assembly. The universal actuating assembly comprises a mounting bracket and a driving member releasably coupled to the driven member. Actuation of the universal actuating assembly translates the driving member and the driven member, moving the latch to the unlatched position. A first interchangeable latch assembly may be swapped with a second interchangeable latch assembly without requiring modification of the universal actuating assembly.

Abstract: A door latch system comprises an interchangeable latch assembly for securing a door to a door frame. The interchangeable latch assembly comprises a head and a latch mounted to the head. The latch is moveable between latched and unlatched positions. A driven member is operatively connected to the latch, the driven member moveable to latch or unlatch the latch. The door latch system further comprises a universal actuating assembly removably couple-able to the interchangeable latch assembly. The universal actuating assembly comprises a mounting bracket and a driving member releasably coupled to the driven member. Actuation of the universal actuating assembly translates the driving member and the driven member, moving the latch to the unlatched position. A first interchangeable latch assembly may be swapped with a second interchangeable latch assembly without requiring modification of the universal actuating assembly.

Abstract: An electric door strike assembly includes a housing and at least one keeper arm. The housing has an opening for admission and retraction of a door latch. The at least one keeper arm has a latch portion and is mounted on the housing. The at least one keeper arm is movable between a closed position, where the opening is occluded by the latch portion, and an open position, where the door latch may be released from the opening. The latch portion may include a bifurcated member having inside and outside legs arranged in spaced parallel relation so as to define a gap therebetween. The gap is configured to receive a latch plate of an interlocking latch mechanism when the at least one keeper arm is in the closed position.

Abstract: A latch dogging assembly comprises an electromagnet and an actuator mounted on a bracket. A guide slide is pivotally mounted on the bracket with a lead block coupled to the guide slide. A guide pin rides along an inner surface of the bracket causing the guide slide to pivot. A pawl is slidably engaged with the guide slide. A guide link includes a post. An armature is mounted to the panic bar and the post engages the pawl. When the actuator is energized to move the lead block, the pawl engages the post to pivot the armature and thereby cause the panic bar to move from the extended position to the depressed position. When the electromagnet is energized the armature is magnetically held preventing reverse pivoting of the guide link. A method for fully retracting a door latch is also provided.

Abstract: A multi-seat gate valve with a pair of sealing members separated by a gate wherein each sealing member is secured between a bottom edge surface on the gate and a pair of locking members until the gate valve is at a fully closed position and proper seating is achieved.

Abstract: A multi-seat gate valve with a pair of sealing members separated by a gate wherein each sealing member is secured between a bottom edge surface on the gate and a pair of locking members until the gate valve is at a fully closed position and proper seating is achieved.

Abstract: A latch dogging assembly comprises an electromagnet and an actuator mounted on a bracket. A guide slide is pivotally mounted on the bracket with a lead block coupled to the guide slide. A guide pin rides along an inner surface of the bracket causing the guide slide to pivot. A pawl is slidably engaged with the guide slide. A guide link includes a post. An armature is mounted to the panic bar and the post engages the pawl. When the actuator is energized to move the lead block, the pawl engages the post to pivot the armature and thereby cause the panic bar to move from the extended position to the depressed position. When the electromagnet is energized the armature is magnetically held preventing reverse pivoting of the guide link. A method for fully retracting a door latch is also provided.

Abstract: The present application is directed to biosensors and methods for detecting a microorganism target in a sample using a mechanically interlocked nucleic acid catanane, wherein an enzyme from the microorganism target or that is activated by a molecule from the microorganism target cleaves a linkage in a first single-stranded nucleic acid ring of the catanane structure, allowing rolling-circle amplification to occur and the presence of rolling-circle amplification products indicates the presence of the microorganism in the sample.

Abstract: An exit device has an overall height when a panic bar of the exit device is in its extended position. The exit device is configured to be received, at least in part, within a recess defined within a body of the door so that the height at which the extended panic bar protrudes beyond an outer surface of the door is reduced.

Abstract: An exit device has an overall height when a panic bar of the exit device is in its extended position. The exit device is configured to be received, at least in part, within a recess defined within a body of the door so that the height at which the extended panic bar protrudes beyond an outer surface of the door is reduced.

Abstract: Provided herein are methods for selection of circular aptamers using a circular nucleic acid library. Also provided are circular aptamers, circular aptamer probes, biosensor systems, and the methods for their use in detecting a microorganism target, or a target molecule present on or generated from a microorganism or a virus in a test sample, including C. difficile glutamate dehydrogenase and methods for determining whether a subject has a C. difficile infection.

Abstract: Described is an approach that takes advantage of rolling circle amplification (RCA) and an RNA-cleaving DNAzyme (RCD) to achieve massive signal amplification for biosensing via a cross-feedback mechanism. An RCA reaction generates copies of an RCD that triggers a reaction cascade designed to generate additional DNA assemblies for RCA. These cross-feedback actions work autonomously to turn limited molecular recognition events into massive amounts of DNA amplicons that can be conveniently detected. This approach was demonstrated for biosensing of a microRNA sequence and a bacterium.

Abstract: A reinforcing assembly may be configured for use in conjunction with a door latch mechanism. The reinforcing assembly is configured to reside within a recess or mortise defined within a body of the door. A body of said door latch mechanism is configured to be received, at least in part, within said reinforcing assembly. The reinforcing assembly includes a body having a back plate and one or more walls thereby defining a latch receiving cavity. At least one fastener is configured to secure the reinforcing assembly to the body of the door. The at least one fastener may be oriented substantially normal to the transverse plane of the door when securing the reinforcing assembly within the body of the door.

Abstract: A latch dogging assembly comprises an electromagnet and an actuator mounted on a bracket. A guide slide is pivotally mounted on the bracket with a lead block coupled to the guide slide. A guide pin rides along an inner surface of the bracket causing the guide slide to pivot. A pawl is slidably engaged with the guide slide. A guide link includes a post. An armature is mounted to the panic bar and the post engages the pawl. When the actuator is energized to move the lead block, the pawl engages the post to pivot the armature and thereby cause the panic bar to move from the extended position to the depressed position. When the electromagnet is energized the armature is magnetically held preventing reverse pivoting of the guide link. A method for fully retracting a door latch is also provided.

Abstract: An electric door strike assembly includes a housing and at least one keeper arm. The housing has an opening for admission and retraction of a door latch. The at least one keeper arm has a latch portion and is mounted on the housing. The at least one keeper arm is movable between a closed position, where the opening is occluded by the latch portion, and an open position, where the door latch may be released from the opening. The latch portion may include a bifurcated member having inside and outside legs arranged in spaced parallel relation so as to define a gap therebetween. The gap is configured to receive a latch plate of an interlocking latch mechanism when the at least one keeper arm is in the closed position.