Abstract: A method for detecting a plurality of analytes in a sample comprises performing a multiplex proximity-based detection assay. The assay utilises pairs of proximity probes with shared hybridisation sites (i.e. hybridisation sites which are shared between different proximity probe pairs). A product comprising a plurality of proximity probe pairs with shared hybridisation sites may be used in the method disclosed herein.

Abstract: The present invention provides a method of detecting multiple analytes in a sample, wherein said analytes have varying levels of abundance in the sample, said method comprising: (i) providing multiple aliquots from the sample; and (ii) in each aliquot, detecting a different subset of the analytes by performing a separate multiplex assay for each aliquot, wherein the analytes in each subset are selected based on their predicted abundance in the sample.

Abstract: The present invention provides a method for detecting a plurality of analytes in a sample, comprising performing a multiplex proximity-based detection assay. The assay utilises pairs of proximity probes with shared hybridisation sites (i.e. hybridisation sites which are shared between different proximity probe pairs). Also provided is a product comprising a plurality of proximity probe pairs with shared hybridisation sites, which may be used in the method disclosed herein.

Abstract: Methods of detecting DNA sequences from multiple pools comprising at least one species of DNA molecule comprise combining the pools to form a combination pool; in the combination pool, generating at least one linear DNA concatemer containing one DNA molecule from each pool, wherein a position of each DNA molecule within the concatemer correlates to the pool from which the DNA molecule originated; and sequencing the concatemers, thereby detecting the DNA sequence of each DNA molecule at each position in each concatemer, wherein each detected DNA sequence is assigned to the pool from which its DNA molecule originated based upon its position within the concatemer.

Abstract: The present invention relates to the identification of novel panels and combinations of biomarkers for ovarian cancer. An in vitro method is provided for detecting, predicting or monitoring ovarian cancer in a subject, wherein said method comprises determining in a sample from said subject the levels of the biomarkers in a panel comprising: TACSTD2, PROK1, MSMB, MUC-16, WFDC2, FR-alpha, and KRT19. More broadly, biomarkers may be selected and used from this list in variations combinations with each other and with other biomarkers. Also provided are sets of reagents for use in such methods.