Patents by Inventor John Brumbaugh

John Brumbaugh has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Method for the fluorescent detection of a DNA sequence in real time
    Patent number: 6197499
    Abstract: The present invention relates to a method of detecting a DNA sequence by means of a DNA:DNA hybrid in real time using fluorescence. The present invention eliminates the need to use radioactive probes to detect the DNA and eliminates the delay needed for autoradiographic exposure of the X-ray to the radioactive label.
    Type: Grant
    Filed: July 27, 1992
    Date of Patent: March 6, 2001
    Assignee: The United States of America as represented by the Department of Health and Human Services
    Inventors: Stephen H. Hughes, Ramesh Kumar, John Brumbaugh
  • DNA sequencing
    Patent number: 6143153
    Abstract: To sequence long strands of DNA, clone strands having lengths longer than 100 bases are, in one embodiment, marked on one end with biotin. These strands are divided into 4 aliquots and each aliquot: (1) is uniquely chemically treated to randomly terminate the strands at the nonbiotinylated end at a selected type of base; and (2) is moved continuously by electrophoresis through a different one of four identical channels. In the one embodiment, the strands are randomly terminated at a selected base type and they are moved into avidin, which due to high affinity, combines with the biotin marked ends of shorter strands before the longer strands are fully resolved in the gel. The avidin is marked with fluorescein, the strands are scanned and the signals are decoded. In another embodiment, the strands are synthesized, with termination at a selected base type and marked either by the above method of by ethidium bromide.
    Type: Grant
    Filed: July 10, 1997
    Date of Patent: November 7, 2000
    Assignee: The Board of Regents of the University of Nebraska
    Inventors: Lyle R. Middendorf, John Brumbaugh
  • DNA sequencing
    Patent number: 6143151
    Abstract: To sequence long strands of DNA, clone strands having lengths longer than 100 bases are, in one embodiment, marked on one end with biotin. These strands are divided into 4 aliquots and each aliquot: (1) is uniquely chemically treated to randomly terminate the strands at the non-biotinylated end at a selected type of base; and (2) is moved continuously by electrophoresis through a different one of four identical channels. In the one embodiment, the strands are randomly terminated at a selected base type and they are moved into avidin, which due to high affinity, combines with the biotin marked ends of shorter strands before the longer strands are fully resolved in the gel. The avidin is marked with fluorescein, the strands are scanned and the signals are decoded. In another embodiment, the strands are synthesized, with termination at a selected base type and marked either by the above method of by ethidium bromide.
    Type: Grant
    Filed: December 11, 1995
    Date of Patent: November 7, 2000
    Assignee: Li-Cor, Inc.
    Inventors: Lyle Richard Middendorf, John Brumbaugh