Patents by Inventor John Buswell
John Buswell has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230022745Abstract: A method of labeling, and optionally enriching, for a population of target RNA molecules in a mixture of RNAs is provided. In some embodiments, the method may comprise (a) adding a label to the 5? end of 5?-diphosphorylated or 5?-triphosphorylated target RNA molecules in a sample by incubating the sample with labeled GTP and a capping enzyme; and (b) optionally enriching for target RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag. The label may be an oligonucleotide, which may further comprise an affinity group attached either internally or at 5? or 3? end of the oligonucleotide where the oligonucleotide label may be added directly, or indirectly via a reaction with a reactive group to the target RNA.Type: ApplicationFiled: September 14, 2022Publication date: January 26, 2023Applicant: New England Biolabs, Inc.Inventors: Ira Schildkraut, Laurence Ettwiller, Ivan R. Correa, JR., George Tzertzinis, John Buswell, Madalee G. Wulf
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Patent number: 11479766Abstract: A method of labeling, and optionally enriching, for a population of target RNA molecules in a mixture of RNAs is provided. In some embodiments, the method may comprise (a) adding a label to the 5? end of 5?-diphosphorylated or 5?-triphosphorylated target RNA molecules in a sample by incubating the sample with labeled GTP and a capping enzyme; and (b) optionally enriching for target RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag. The label may be an oligonucleotide, which may further comprise an affinity group attached either internally or at 5? or 3? end of the oligonucleotide where the oligonucleotide label may be added directly, or indirectly via a reaction with a reactive group to the target RNA.Type: GrantFiled: February 28, 2018Date of Patent: October 25, 2022Assignee: New England Biolabs, Inc.Inventors: Ira Schildkraut, Laurence Ettwiller, Ivan R. Correa, Jr., George Tzertzinis, John Buswell, Madalee G. Wulf
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Publication number: 20180195061Abstract: A method of labeling, and optionally enriching, for a population of target RNA molecules in a mixture of RNAs is provided. In some embodiments, the method may comprise (a) adding a label to the 5? end of 5?-diphosphorylated or 5?-triphosphorylated target RNA molecules in a sample by incubating the sample with labeled GTP and a capping enzyme; and (b) optionally enriching for target RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag. The label may be an oligonucleotide, which may further comprise an affinity group attached either internally or at 5? or 3? end of the oligonucleotide where the oligonucleotide label may be added directly, or indirectly via a reaction with a reactive group to the target RNA.Type: ApplicationFiled: February 28, 2018Publication date: July 12, 2018Applicant: New England Biolabs, Inc.Inventors: Ira Schildkraut, Laurence Ettwiller, Ivan R. Correa, Jr., George Tzertzinis, John Buswell, Madalee G. Wulf
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Patent number: 9964548Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.Type: GrantFiled: December 28, 2016Date of Patent: May 8, 2018Assignee: New England Biolabs, Inc.Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
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Publication number: 20170138959Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.Type: ApplicationFiled: December 28, 2016Publication date: May 18, 2017Applicant: New England Biolabs, Inc.Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
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Publication number: 20150346194Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.Type: ApplicationFiled: May 29, 2015Publication date: December 3, 2015Applicant: NEW ENGLAND BIOLABS, INC.Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
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Patent number: 8824485Abstract: Packet processing logic of a host system's virtualization manager detects packets on the ingress or the egress path to/from a virtual port having three bitmap arrays for processing packets within a virtual local area network (VLAN). The logic checks the VLAN identifier (VID) of the packet to determine, based on an offset position within the corresponding bitmap array, whether the port supports the VLAN. Both the ingress array offset position and egress array offset positions correspond to the value of the VID, and are set within the specific bitmap array during configuration of the VLAN on the port. When the VLAN is supported by the port, the logic enables the packet to be processed by the port. Otherwise, the logic discards the packet. A strip bitmap array indicates when a packet's VID should be removed prior to forwarding the packet on the egress of a port (or destination port).Type: GrantFiled: September 15, 2012Date of Patent: September 2, 2014Assignee: International Business Machines CorporationInventors: Amitabha Biswas, John Buswell, Dayavanti G. Kamath, Jayakrishna Kidambi, Vijoy Pandey, Dorit Rond
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Patent number: 8793685Abstract: A technique for operating a virtual switch includes determining network connection requirements for virtual machines controlled by a virtual machine monitor. Resources available, for processing data traffic of the virtual machines, are also determined. Finally, based on the network connection requirements and the resources available, a port of a virtual switch is selected to operate as a virtual Ethernet bridge or a virtual Ethernet port aggregator.Type: GrantFiled: May 13, 2011Date of Patent: July 29, 2014Assignee: International Business Machines CorporationInventors: Jayakrishna Kidambi, Amitabha Biswas, John Buswell, Dorit Rond
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Patent number: 8793687Abstract: A technique for operating a virtual switch includes determining network connection requirements for virtual machines controlled by a virtual machine monitor. Resources available, for processing data traffic of the virtual machines, are also determined. Finally, based on the network connection requirements and the resources available, a port of a virtual switch is selected to operate as a virtual Ethernet bridge or a virtual Ethernet port aggregator.Type: GrantFiled: May 8, 2012Date of Patent: July 29, 2014Assignee: International Business Machines CorporationInventors: Jayakrishna Kidambi, Amitabha Biswas, John Buswell, Dorit Rond
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Patent number: 8670450Abstract: Packet processing logic of a host system's virtualization manager detects packets on the ingress or the egress path to/from a virtual port having three bitmap arrays for processing packets within a virtual local area network (VLAN). The logic checks the VLAN identifier (VID) of the packet to determine, based on an offset position within the corresponding bitmap array, whether the port supports the VLAN. Both the ingress array offset position and egress array offset positions correspond to the value of the VID, and are set within the specific bitmap array during configuration of the VLAN on the port. When the VLAN is supported by the port, the logic enables the packet to be processed by the port. Otherwise, the logic discards the packet. A strip bitmap array indicates when a packet's VID should be removed prior to forwarding the packet on the egress of a port (or destination port).Type: GrantFiled: May 13, 2011Date of Patent: March 11, 2014Assignee: International Business Machines CorporationInventors: Amitabha Biswas, John Buswell, Dayavanti G. Kamath, Jayakrishna Kidambi, Vijoy Pandey, Dorit Rond
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Publication number: 20130010799Abstract: Packet processing logic of a host system's virtualization manager detects packets on the ingress or the egress path to/from a virtual port having three bitmap arrays for processing packets within a virtual local area network (VLAN). The logic checks the VLAN identifier (VID) of the packet to determine, based on an offset position within the corresponding bitmap array, whether the port supports the VLAN. Both the ingress array offset position and egress array offset positions correspond to the value of the VID, and are set within the specific bitmap array during configuration of the VLAN on the port. When the VLAN is supported by the port, the logic enables the packet to be processed by the port. Otherwise, the logic discards the packet. A strip bitmap array indicates when a packet's VID should be removed prior to forwarding the packet on the egress of a port (or destination port).Type: ApplicationFiled: September 15, 2012Publication date: January 10, 2013Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Amitabha Biwas, John Buswell, Dayavanti G. Kamath, Jayakrishna Kidambi, Vijoy Pandey, Dorit Rond
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Publication number: 20120287936Abstract: Packet processing logic of a host system's virtualization manager detects packets on the ingress or the egress path to/from a virtual port having three bitmap arrays for processing packets within a virtual local area network (VLAN). The logic checks the VLAN identifier (VID) of the packet to determine, based on an offset position within the corresponding bitmap array, whether the port supports the VLAN. Both the ingress array offset position and egress array offset positions correspond to the value of the VID, and are set within the specific bitmap array during configuration of the VLAN on the port. When the VLAN is supported by the port, the logic enables the packet to be processed by the port. Otherwise, the logic discards the packet. A strip bitmap array indicates when a packet's VID should be removed prior to forwarding the packet on the egress of a port (or destination port).Type: ApplicationFiled: May 13, 2011Publication date: November 15, 2012Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Amitabha Biswas, John Buswell, Dayavanti G. Kamath, Jayakrishna Kidambi, Vijoy Pandey, Dorit Rond
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Publication number: 20120291029Abstract: A technique for operating a virtual switch includes determining network connection requirements for virtual machines controlled by a virtual machine monitor. Resources available, for processing data traffic of the virtual machines, are also determined. Finally, based on the network connection requirements and the resources available, a port of a virtual switch is selected to operate as a virtual Ethernet bridge or a virtual Ethernet port aggregator.Type: ApplicationFiled: May 8, 2012Publication date: November 15, 2012Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: JAYAKRISHNA KIDAMBI, AMITABHA BISWAS, JOHN BUSWELL, DORIT ROND
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Publication number: 20120291025Abstract: A technique for operating a virtual switch includes determining network connection requirements for virtual machines controlled by a virtual machine monitor. Resources available, for processing data traffic of the virtual machines, are also determined. Finally, based on the network connection requirements and the resources available, a port of a virtual switch is selected to operate as a virtual Ethernet bridge or a virtual Ethernet port aggregator.Type: ApplicationFiled: May 13, 2011Publication date: November 15, 2012Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATIONInventors: Jayakrishna Kidambi, Amitabha Biswas, John Buswell, Dorit Rond
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Patent number: 4889807Abstract: The invention relates to new microorganisms of the Phanerochaete chrysosporium strain, which are useful especially for the production of lignin-degrading enzyme.These new microorganisms are deposited under nos. C.N.C.M.-I-398 and C.N.C.M.-I-399.Type: GrantFiled: December 10, 1985Date of Patent: December 26, 1989Assignee: Institut National de la Recherche AronomiqueInventors: John A. Buswell, Etienne Odier