Patents by Inventor John Buswell

John Buswell has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20230022745
    Abstract: A method of labeling, and optionally enriching, for a population of target RNA molecules in a mixture of RNAs is provided. In some embodiments, the method may comprise (a) adding a label to the 5? end of 5?-diphosphorylated or 5?-triphosphorylated target RNA molecules in a sample by incubating the sample with labeled GTP and a capping enzyme; and (b) optionally enriching for target RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag. The label may be an oligonucleotide, which may further comprise an affinity group attached either internally or at 5? or 3? end of the oligonucleotide where the oligonucleotide label may be added directly, or indirectly via a reaction with a reactive group to the target RNA.
    Type: Application
    Filed: September 14, 2022
    Publication date: January 26, 2023
    Applicant: New England Biolabs, Inc.
    Inventors: Ira Schildkraut, Laurence Ettwiller, Ivan R. Correa, JR., George Tzertzinis, John Buswell, Madalee G. Wulf
  • Patent number: 11479766
    Abstract: A method of labeling, and optionally enriching, for a population of target RNA molecules in a mixture of RNAs is provided. In some embodiments, the method may comprise (a) adding a label to the 5? end of 5?-diphosphorylated or 5?-triphosphorylated target RNA molecules in a sample by incubating the sample with labeled GTP and a capping enzyme; and (b) optionally enriching for target RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag. The label may be an oligonucleotide, which may further comprise an affinity group attached either internally or at 5? or 3? end of the oligonucleotide where the oligonucleotide label may be added directly, or indirectly via a reaction with a reactive group to the target RNA.
    Type: Grant
    Filed: February 28, 2018
    Date of Patent: October 25, 2022
    Assignee: New England Biolabs, Inc.
    Inventors: Ira Schildkraut, Laurence Ettwiller, Ivan R. Correa, Jr., George Tzertzinis, John Buswell, Madalee G. Wulf
  • Publication number: 20180195061
    Abstract: A method of labeling, and optionally enriching, for a population of target RNA molecules in a mixture of RNAs is provided. In some embodiments, the method may comprise (a) adding a label to the 5? end of 5?-diphosphorylated or 5?-triphosphorylated target RNA molecules in a sample by incubating the sample with labeled GTP and a capping enzyme; and (b) optionally enriching for target RNA comprising the affinity tag-labeled GMP using an affinity matrix that binds to the affinity tag. The label may be an oligonucleotide, which may further comprise an affinity group attached either internally or at 5? or 3? end of the oligonucleotide where the oligonucleotide label may be added directly, or indirectly via a reaction with a reactive group to the target RNA.
    Type: Application
    Filed: February 28, 2018
    Publication date: July 12, 2018
    Applicant: New England Biolabs, Inc.
    Inventors: Ira Schildkraut, Laurence Ettwiller, Ivan R. Correa, Jr., George Tzertzinis, John Buswell, Madalee G. Wulf
  • Patent number: 9964548
    Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.
    Type: Grant
    Filed: December 28, 2016
    Date of Patent: May 8, 2018
    Assignee: New England Biolabs, Inc.
    Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
  • Publication number: 20170138959
    Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.
    Type: Application
    Filed: December 28, 2016
    Publication date: May 18, 2017
    Applicant: New England Biolabs, Inc.
    Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
  • Publication number: 20150346194
    Abstract: Compositions and methods are provided for efficiently preparing a completely deglycosylated antibody where efficiency is measured in relative amounts of reagents in soluble or lyophilized form, and time and temperature of the reaction. Compositions and methods are also provided for separating substantially all N-linked glycans from a glycosylated antibody and for preserving functionality of the antibody. The methods are compatible with glycan labeling and protease digestion without the need for prior purification steps.
    Type: Application
    Filed: May 29, 2015
    Publication date: December 3, 2015
    Applicant: NEW ENGLAND BIOLABS, INC.
    Inventors: Paula Magnelli, Ellen Guthrie, Christopher H. Taron, Ming-Qun Xu, John Buswell
  • Patent number: 8824485
    Abstract: Packet processing logic of a host system's virtualization manager detects packets on the ingress or the egress path to/from a virtual port having three bitmap arrays for processing packets within a virtual local area network (VLAN). The logic checks the VLAN identifier (VID) of the packet to determine, based on an offset position within the corresponding bitmap array, whether the port supports the VLAN. Both the ingress array offset position and egress array offset positions correspond to the value of the VID, and are set within the specific bitmap array during configuration of the VLAN on the port. When the VLAN is supported by the port, the logic enables the packet to be processed by the port. Otherwise, the logic discards the packet. A strip bitmap array indicates when a packet's VID should be removed prior to forwarding the packet on the egress of a port (or destination port).
    Type: Grant
    Filed: September 15, 2012
    Date of Patent: September 2, 2014
    Assignee: International Business Machines Corporation
    Inventors: Amitabha Biswas, John Buswell, Dayavanti G. Kamath, Jayakrishna Kidambi, Vijoy Pandey, Dorit Rond
  • Patent number: 8793685
    Abstract: A technique for operating a virtual switch includes determining network connection requirements for virtual machines controlled by a virtual machine monitor. Resources available, for processing data traffic of the virtual machines, are also determined. Finally, based on the network connection requirements and the resources available, a port of a virtual switch is selected to operate as a virtual Ethernet bridge or a virtual Ethernet port aggregator.
    Type: Grant
    Filed: May 13, 2011
    Date of Patent: July 29, 2014
    Assignee: International Business Machines Corporation
    Inventors: Jayakrishna Kidambi, Amitabha Biswas, John Buswell, Dorit Rond
  • Patent number: 8793687
    Abstract: A technique for operating a virtual switch includes determining network connection requirements for virtual machines controlled by a virtual machine monitor. Resources available, for processing data traffic of the virtual machines, are also determined. Finally, based on the network connection requirements and the resources available, a port of a virtual switch is selected to operate as a virtual Ethernet bridge or a virtual Ethernet port aggregator.
    Type: Grant
    Filed: May 8, 2012
    Date of Patent: July 29, 2014
    Assignee: International Business Machines Corporation
    Inventors: Jayakrishna Kidambi, Amitabha Biswas, John Buswell, Dorit Rond
  • Patent number: 8670450
    Abstract: Packet processing logic of a host system's virtualization manager detects packets on the ingress or the egress path to/from a virtual port having three bitmap arrays for processing packets within a virtual local area network (VLAN). The logic checks the VLAN identifier (VID) of the packet to determine, based on an offset position within the corresponding bitmap array, whether the port supports the VLAN. Both the ingress array offset position and egress array offset positions correspond to the value of the VID, and are set within the specific bitmap array during configuration of the VLAN on the port. When the VLAN is supported by the port, the logic enables the packet to be processed by the port. Otherwise, the logic discards the packet. A strip bitmap array indicates when a packet's VID should be removed prior to forwarding the packet on the egress of a port (or destination port).
    Type: Grant
    Filed: May 13, 2011
    Date of Patent: March 11, 2014
    Assignee: International Business Machines Corporation
    Inventors: Amitabha Biswas, John Buswell, Dayavanti G. Kamath, Jayakrishna Kidambi, Vijoy Pandey, Dorit Rond
  • Publication number: 20130010799
    Abstract: Packet processing logic of a host system's virtualization manager detects packets on the ingress or the egress path to/from a virtual port having three bitmap arrays for processing packets within a virtual local area network (VLAN). The logic checks the VLAN identifier (VID) of the packet to determine, based on an offset position within the corresponding bitmap array, whether the port supports the VLAN. Both the ingress array offset position and egress array offset positions correspond to the value of the VID, and are set within the specific bitmap array during configuration of the VLAN on the port. When the VLAN is supported by the port, the logic enables the packet to be processed by the port. Otherwise, the logic discards the packet. A strip bitmap array indicates when a packet's VID should be removed prior to forwarding the packet on the egress of a port (or destination port).
    Type: Application
    Filed: September 15, 2012
    Publication date: January 10, 2013
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Amitabha Biwas, John Buswell, Dayavanti G. Kamath, Jayakrishna Kidambi, Vijoy Pandey, Dorit Rond
  • Publication number: 20120287936
    Abstract: Packet processing logic of a host system's virtualization manager detects packets on the ingress or the egress path to/from a virtual port having three bitmap arrays for processing packets within a virtual local area network (VLAN). The logic checks the VLAN identifier (VID) of the packet to determine, based on an offset position within the corresponding bitmap array, whether the port supports the VLAN. Both the ingress array offset position and egress array offset positions correspond to the value of the VID, and are set within the specific bitmap array during configuration of the VLAN on the port. When the VLAN is supported by the port, the logic enables the packet to be processed by the port. Otherwise, the logic discards the packet. A strip bitmap array indicates when a packet's VID should be removed prior to forwarding the packet on the egress of a port (or destination port).
    Type: Application
    Filed: May 13, 2011
    Publication date: November 15, 2012
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Amitabha Biswas, John Buswell, Dayavanti G. Kamath, Jayakrishna Kidambi, Vijoy Pandey, Dorit Rond
  • Publication number: 20120291029
    Abstract: A technique for operating a virtual switch includes determining network connection requirements for virtual machines controlled by a virtual machine monitor. Resources available, for processing data traffic of the virtual machines, are also determined. Finally, based on the network connection requirements and the resources available, a port of a virtual switch is selected to operate as a virtual Ethernet bridge or a virtual Ethernet port aggregator.
    Type: Application
    Filed: May 8, 2012
    Publication date: November 15, 2012
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: JAYAKRISHNA KIDAMBI, AMITABHA BISWAS, JOHN BUSWELL, DORIT ROND
  • Publication number: 20120291025
    Abstract: A technique for operating a virtual switch includes determining network connection requirements for virtual machines controlled by a virtual machine monitor. Resources available, for processing data traffic of the virtual machines, are also determined. Finally, based on the network connection requirements and the resources available, a port of a virtual switch is selected to operate as a virtual Ethernet bridge or a virtual Ethernet port aggregator.
    Type: Application
    Filed: May 13, 2011
    Publication date: November 15, 2012
    Applicant: INTERNATIONAL BUSINESS MACHINES CORPORATION
    Inventors: Jayakrishna Kidambi, Amitabha Biswas, John Buswell, Dorit Rond
  • Patent number: 4889807
    Abstract: The invention relates to new microorganisms of the Phanerochaete chrysosporium strain, which are useful especially for the production of lignin-degrading enzyme.These new microorganisms are deposited under nos. C.N.C.M.-I-398 and C.N.C.M.-I-399.
    Type: Grant
    Filed: December 10, 1985
    Date of Patent: December 26, 1989
    Assignee: Institut National de la Recherche Aronomique
    Inventors: John A. Buswell, Etienne Odier