Patents by Inventor John C. Gerdes
John C. Gerdes has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 10850276Abstract: Systems and methods that enable the rapid identification of target molecules present in a sample at low concentrations is provided. The system includes a sample volume, and a detection structure that is connected to the sample volume by a conduit. The detection structure includes a microfluidic chip that defines a plurality of fluid channels. The walls of the fluid channels are formed from or covered with a metal oxide to which target molecules attach. After the sample volume has been passed through the detection structure, and in particular through the channels, visible microparticles are passed through the detection structure. The visible microparticles are configured to have an affinity for the target molecules. The microfluidic chip is then imaged to detect visible microparticles, and to thereby obtain information regarding the presence of the target molecules.Type: GrantFiled: February 26, 2018Date of Patent: December 1, 2020Assignee: VISUGEN GLOBAL LLCInventors: John C. Gerdes, Kirsten L. Nelson, Kris Buchanan
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Publication number: 20180243738Abstract: Systems and methods that enable the rapid identification of target molecules present in a sample at low concentrations is provided. The system includes a sample volume, and a detection structure that is connected to the sample volume by a conduit. The detection structure includes a microfluidic chip that defines a plurality of fluid channels. The walls of the fluid channels are formed from or covered with a metal oxide to which target molecules attach. After the sample volume has been passed through the detection structure, and in particular through the channels, visible microparticles are passed through the detection structure. The visible microparticles are configured to have an affinity for the target molecules. The microfluidic chip is then imaged to detect visible microparticles, and to thereby obtain information regarding the presence of the target molecules.Type: ApplicationFiled: February 26, 2018Publication date: August 30, 2018Inventors: John C. Gerdes, Kirsten L. Nelson, Kris Buchanan
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Patent number: 9481907Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: June 12, 2014Date of Patent: November 1, 2016Assignee: Applied Biosystems, LLCInventors: John C. Gerdes, Elaine Best, Jeffrey M. Marmaro
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Patent number: 9206475Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: January 5, 2015Date of Patent: December 8, 2015Assignee: APPLIED BIOSYSTEMS, LLCInventors: John C. Gerdes, Elaine Best, Jeffrey M. Marmaro
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Patent number: 8304214Abstract: A two-step multiplex amplification reaction includes a first step which truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: November 21, 2007Date of Patent: November 6, 2012Assignee: Applied Biosystems, LLCInventors: John C. Gerdes, Elaine A. Best, Jeffrey M. Marmaro
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Patent number: 7531328Abstract: The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: July 7, 2005Date of Patent: May 12, 2009Assignee: Applied Biosystem, LLCInventors: John C. Gerdes, Elaine Best, Jeffery M. Marmaro
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Patent number: 7361471Abstract: This invention is directed to a process for tightly binding nucleic acid to solid phase and corresponding processes for the utilization thereof. Nucleic acid is bound to solid phase matrices exhibiting sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids from a sample. These processes include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method is described for virus extraction, purification, and solid phase amplification from large volume plasma specimens.Type: GrantFiled: May 18, 2006Date of Patent: April 22, 2008Assignee: Applera CorporationInventors: John C. Gerdes, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Patent number: 7087414Abstract: The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100–1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: GrantFiled: May 19, 2003Date of Patent: August 8, 2006Assignee: Applera CorporationInventors: John C. Gerdes, Elaine Best, Jeffery M. Marmaro
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Patent number: 7087387Abstract: This invention provides a kit comprising a substrate having a surface coated with a solid phase matrix for nucleic acid manipulation. The solid phase matrix exhibits sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids in a sample. The manipulations include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method for virus extraction, purification, and solid phase amplification from large volume plasma specimens is described.Type: GrantFiled: October 21, 2003Date of Patent: August 8, 2006Assignee: Applera CorporationInventors: John C. Gerdes, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Patent number: 6872527Abstract: This invention is directed to a process for tightly binding nucleic acid to solid phase and corresponding processes for the utilization thereof. Nucleic acid is bound to solid phase matrices exhibiting sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids from a sample. These processes include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method is described for virus extraction, purification, and solid phase amplification from large volume plasma specimens.Type: GrantFiled: August 31, 2001Date of Patent: March 29, 2005Assignee: XTRANA, Inc.Inventors: John C. Gerdes, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Publication number: 20040110167Abstract: The invention provides a complete, one-step, fully functional, ready to use lateral flow assay device for the rapid, accurate detection of a target nucleic acid in a fluid sample, wherein the device contains all reagents necessary for the assay in an anhydrous format. The device comprises a sample receiving zone, a labeling zone, and a capture zone. The sample receiving zone may contain one or more oligonucleotides coupled to binding partners and reversibly bound to the capture zone membrane, the labeling zone comprises a visible moiety coupled to a ligand specific for one of the binding partners and reversibly bound to the labeling zone membrane, and the capture zone comprises an capture moiety specific for the second binding partner and immobilized on the capture zone membrane.Type: ApplicationFiled: April 14, 2003Publication date: June 10, 2004Inventors: John C. Gerdes, Roy R. Mondesire, Lara A. Hansen
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Publication number: 20040091925Abstract: This invention provides a kit comprising a substrate having a surface coated with a solid phase matrix for nucleic acid manipulation. The solid phase matrix exhibits sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids in a sample. The manipulations include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method for virus extraction, purification, and solid phase amplification from large volume plasma specimens is described.Type: ApplicationFiled: October 21, 2003Publication date: May 13, 2004Inventors: John C. Gerdes, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Publication number: 20030224437Abstract: The present invention provides a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid. In particular, nucleic acid sequences that uniquely identify E. Coli were identified using the multiplex amplification method.Type: ApplicationFiled: May 19, 2003Publication date: December 4, 2003Inventors: John C. Gerdes, Elaine Best, Jeffery M. Marmaro
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Patent number: 6649378Abstract: Self-contained devices are described that integrate nucleic acid extraction, specific target amplification and detection into a single device. This integration permits rapid and accurate nucleic acid sequence detection. The invention may be used, for example, in the screening for nucleic acid sequences which may be indicative of genetic defects or contagious diseases, as well as for monitoring efficacy in the treatment of contagious diseases.Type: GrantFiled: November 2, 2000Date of Patent: November 18, 2003Assignee: Xtrana, Inc.Inventors: Diane L. Kozwich, John C. Gerdes
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Patent number: 6605451Abstract: The present invention pertains to methods, reagents, compositions, kits, and instruments for use in simultaneously amplifying multiple targets. In particular, this invention is based on the discovery of a two-step multiplex amplification reaction wherein the first step truncates the standard initial multiplex amplification round to “boost” the sample copy number by only a 100-1000 fold increase in the target. Following the first step the product is divided into optimized secondary single amplification reactions, each containing one of the primer sets that were used previously in the first or multiplexed booster step. The booster step can occur using an aqueous target nucleic acid or using a solid phase archived nucleic acid.Type: GrantFiled: June 6, 2000Date of Patent: August 12, 2003Assignee: Xtrana, Inc.Inventors: Jeffery M. Marmaro, John C. Gerdes
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Publication number: 20020132242Abstract: This invention is directed to a process for tightly binding nucleic acid to solid phase and corresponding processes for the utilization thereof. Nucleic acid is bound to solid phase matrices exhibiting sufficient hydrophilicity and electropositivity to tightly bind the nucleic acids from a sample. These processes include nucleic acid (double or single stranded DNA and RNA) capture from high volume and/or low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The tightly bound nucleic acid may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method is described for virus extraction, purification, and solid phase amplification from large volume plasma specimens.Type: ApplicationFiled: August 31, 2001Publication date: September 19, 2002Inventors: John C. Gerdes, Jeffery M. Marmaro, Jeffrey T. Ives, Christopher A. Roehl
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Patent number: 6291166Abstract: This invention is directed to a process for irreversibly binding nucleic acid to solid phase and corresponding processes for the utilization thereof. Nucleic acid is bound to solid phase matrixes exhibiting sufficient hydrophilicity and electropositivity to irreversibly bind the nucleic acids from a sample. These processes include nucleic acid (double or single stranded DNA and RNA) capture from high volume:low concentration specimens, buffer changes, washes, and volume reductions, and enable the interface of solid phase bound nucleic acid with enzyme, hybridization or amplification strategies. The invention, solid phase irreversibly bound nucleic acid, may be used, for example, in repeated analyses to confirm results or test additional genes in both research and commercial applications. Further, a method is described for virus extraction, purification, and solid phase amplification from large volume plasma specimens.Type: GrantFiled: April 16, 1998Date of Patent: September 18, 2001Assignee: Xtrana, Inc.Inventors: John C. Gerdes, Jeffrey M. Marmaro, Christopher A. Roehl
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Patent number: 6258543Abstract: This invention is directed to methods for the quantitative measurement of specific gene expression levels in biological samples. In one embodiment, methods for the quantitative monitoring of gene expression without either co-amplification of an added template or use of an endogenous constitutive transcript are provided. The former involves a duplex amplification reaction in which a single set of primers is used to amplify both genomic DNA and expressed mRNA from the same gene sequence. These primers are targeted for sequences flanking the splice junction and intron sequences for the mRNA and DNA respectively. By their use, any suitable nucleic acid amplification technology yields mRNA and DNA amplimers which are distinguishable by length and sequence heterogeneity.Type: GrantFiled: September 17, 1999Date of Patent: July 10, 2001Assignee: Xtrana, Inc.Inventors: John C. Gerdes, Jeffrey M. Marmaro
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Patent number: 6153425Abstract: A self-contained device is described that integrates nucleic acid extraction, specific target amplification and detection into a single device. This integration permits rapid and accurate nucleic acid sequence detection. The invention may be used, for example, in the screening for nucleic acid sequences which may be indicative of genetic defects or contagious diseases, as well as for monitoring efficacy in the treatment of contagious diseases.Type: GrantFiled: August 27, 1998Date of Patent: November 28, 2000Assignee: Xtrana, Inc.Inventors: Diane L. Kozwich, John C. Gerdes
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Patent number: 6063568Abstract: A method for the quantitative monitoring of gene expression without either co-amplification of an added template or use of an endogenous constitutive transcript is provided. The process involves a duplex amplification reaction in which a single set of primers is used to amplify both genomic DNA and expressed mRNA from the same gene sequence. These primers are targeted for sequences flanking the splice junction/intron sequences for the mRNA/DNA respectively. By their use, any suitable nucleic acid amplification technology yields mRNA and DNA amplimers which are distinguishable by length and sequence heterogeneity. These amplimers are present in the final amplification reaction in ratios which are dependent upon the ratios of the expressed mRNA to the DNA in the sample, allowing the quantitation of mRNA in a sample which is normalized to the number of copies of genomic DNA since the genomic DNA acts as the internal quantitation standard, and in effect yields the amount of mRNA per cell.Type: GrantFiled: May 2, 1997Date of Patent: May 16, 2000Assignee: Molecular Innovations, Inc.Inventors: John C. Gerdes, Jeffrey M. Marmaro